RHDF2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RHDF2_HUMAN
• UniProt AC: Q6PJF5
• Protein Name: Inactive rhomboid protein 2
• Gene Name: RHBDF2
• Organism: Homo sapiens (Human).
• Sequence Length: 856
• Subcellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane .
• Protein Description: Rhomboid protease-like protein which has no protease activity but regulates the secretion of several ligands of the epidermal growth factor receptor. Indirectly activates the epidermal growth factor receptor signaling pathway and may thereby regulate sleep, cell survival, proliferation and migration (By similarity)..
• Protein Sequence: MASADKNGGSVSSVSSSRLQSRKPPNLSITIPPPEKETQAPGEQDSMLPEGFQNRRLKKSQPRTWAAHTTACPPSFLPKRKNPAYLKSVSLQEPRSRWQESSEKRPGFRRQASLSQSIRKGAAQWFGVSGDWEGQRQQWQRRSLHHCSMRYGRLKASCQRDLELPSQEAPSFQGTESPKPCKMPKIVDPLARGRAFRHPEEMDRPHAPHPPLTPGVLSLTSFTSVRSGYSHLPRRKRMSVAHMSLQAAAALLKGRSVLDATGQRCRVVKRSFAFPSFLEEDVVDGADTFDSSFFSKEEMSSMPDDVFESPPLSASYFRGIPHSASPVSPDGVQIPLKEYGRAPVPGPRRGKRIASKVKHFAFDRKKRHYGLGVVGNWLNRSYRRSISSTVQRQLESFDSHRPYFTYWLTFVHVIITLLVICTYGIAPVGFAQHVTTQLVLRNKGVYESVKYIQQENFWVGPSSIDLIHLGAKFSPCIRKDGQIEQLVLRERDLERDSGCCVQNDHSGCIQTQRKDCSETLATFVKWQDDTGPPMDKSDLGQKRTSGAVCHQDPRTCEEPASSGAHIWPDDITKWPICTEQARSNHTGFLHMDCEIKGRPCCIGTKGSCEITTREYCEFMHGYFHEEATLCSQVHCLDKVCGLLPFLNPEVPDQFYRLWLSLFLHAGVVHCLVSVVFQMTILRDLEKLAGWHRIAIIFILSGITGNLASAIFLPYRAEVGPAGSQFGLLACLFVELFQSWPLLERPWKAFLNLSAIVLFLFICGLLPWIDNIAHIFGFLSGLLLAFAFLPYITFGTSDKYRKRALILVSLLAFAGLFAALVLWLYIYPINWPWIEHLTCFPFTSRFCEKYELDQVLH

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	SADKNGGSVSSVSSS CCCCCCCCCCCCCHH	21.89	23090842
12	Phosphorylation	DKNGGSVSSVSSSRL CCCCCCCCCCCHHHC	27.07	23090842
13	Phosphorylation	KNGGSVSSVSSSRLQ CCCCCCCCCCHHHCC	24.70	23090842
15	Phosphorylation	GGSVSSVSSSRLQSR CCCCCCCCHHHCCCC	25.25	23090842
16	Phosphorylation	GSVSSVSSSRLQSRK CCCCCCCHHHCCCCC	19.81	23090842
17	Phosphorylation	SVSSVSSSRLQSRKP CCCCCCHHHCCCCCC	29.51	23090842
21	Phosphorylation	VSSSRLQSRKPPNLS CCHHHCCCCCCCCCE	47.03	23090842
28	Phosphorylation	SRKPPNLSITIPPPE CCCCCCCEEECCCCC	25.07	23312004
30	Phosphorylation	KPPNLSITIPPPEKE CCCCCEEECCCCCCC	24.85	23312004
46	Phosphorylation	QAPGEQDSMLPEGFQ CCCCCCCCCCCHHHH	23.16	22210691
52	Ubiquitination	DSMLPEGFQNRRLKK CCCCCHHHHHCCCCC	5.77	29967540
58	Ubiquitination	GFQNRRLKKSQPRTW HHHHCCCCCCCCCCH	48.87	29967540
61 (in isoform 2)	Phosphorylation	-	40.13	29507054
61	Phosphorylation	NRRLKKSQPRTWAAH HCCCCCCCCCCHHHC	40.13	32645325
75	Ubiquitination	HTTACPPSFLPKRKN CCCCCCHHHCCCCCC	25.26	29967540
81	Ubiquitination	PSFLPKRKNPAYLKS HHHCCCCCCCHHHHC	73.70	-
87	Ubiquitination	RKNPAYLKSVSLQEP CCCCHHHHCCCCCCC	35.81	-
88	Phosphorylation	KNPAYLKSVSLQEPR CCCHHHHCCCCCCCH	17.84	30266825
90	Phosphorylation	PAYLKSVSLQEPRSR CHHHHCCCCCCCHHH	31.27	25159151
96	Phosphorylation	VSLQEPRSRWQESSE CCCCCCHHHHHHHHH	48.89	28102081
101	Phosphorylation	PRSRWQESSEKRPGF CHHHHHHHHHCCCCH	28.79	28857561
102	Phosphorylation	RSRWQESSEKRPGFR HHHHHHHHHCCCCHH	46.51	28857561
104	Ubiquitination	RWQESSEKRPGFRRQ HHHHHHHCCCCHHHH	66.88	29967540
113	Phosphorylation	PGFRRQASLSQSIRK CCHHHHHHHHHHHHH	21.88	23401153
115	Phosphorylation	FRRQASLSQSIRKGA HHHHHHHHHHHHHCC	21.27	30266825
117	Phosphorylation	RQASLSQSIRKGAAQ HHHHHHHHHHHCCHH	22.45	20164059
120	Methylation	SLSQSIRKGAAQWFG HHHHHHHHCCHHHHC	51.75	115976631
129	Phosphorylation	AAQWFGVSGDWEGQR CHHHHCCCCCCCHHH	31.26	29507054
143	Phosphorylation	RQQWQRRSLHHCSMR HHHHHHHHHHHHHHH	33.95	27251275
148	Phosphorylation	RRSLHHCSMRYGRLK HHHHHHHHHHHHHHH	11.26	28348404
150	Ubiquitination	SLHHCSMRYGRLKAS HHHHHHHHHHHHHHH	17.92	29967540
166	Phosphorylation	QRDLELPSQEAPSFQ HCCCCCCCCCCCCCC	53.96	23927012
171	Phosphorylation	LPSQEAPSFQGTESP CCCCCCCCCCCCCCC	36.93	23401153
175	Phosphorylation	EAPSFQGTESPKPCK CCCCCCCCCCCCCCC	23.62	23401153
177	Phosphorylation	PSFQGTESPKPCKMP CCCCCCCCCCCCCCC	37.75	30266825
179	Ubiquitination	FQGTESPKPCKMPKI CCCCCCCCCCCCCCC	72.78	29967540
192	Methylation	KIVDPLARGRAFRHP CCCCHHHCCCCCCCH	42.70	54558769
213	Phosphorylation	HAPHPPLTPGVLSLT CCCCCCCCCCEEEEC	24.62	24719451
227	Phosphorylation	TSFTSVRSGYSHLPR CCCCCCCCCCCCCCH	39.67	30266825
229	Phosphorylation	FTSVRSGYSHLPRRK CCCCCCCCCCCCHHH	8.31	30266825
230	Phosphorylation	TSVRSGYSHLPRRKR CCCCCCCCCCCHHHC	23.29	30266825
239	Phosphorylation	LPRRKRMSVAHMSLQ CCHHHCCCHHHHHHH	21.86	22617229
244	Phosphorylation	RMSVAHMSLQAAAAL CCCHHHHHHHHHHHH	13.89	22985185
256	Phosphorylation	AALLKGRSVLDATGQ HHHHCCCHHHHCCCC	35.38	28857561
261	Phosphorylation	GRSVLDATGQRCRVV CCHHHHCCCCCEEEE	33.25	27470641
271	Phosphorylation	RCRVVKRSFAFPSFL CEEEEEHHHCCCCCC	18.40	29523821
276	Phosphorylation	KRSFAFPSFLEEDVV EHHHCCCCCCCCCCC	35.69	29523821
288	Phosphorylation	DVVDGADTFDSSFFS CCCCCCCCCCHHHCC	28.89	-
300	Phosphorylation	FFSKEEMSSMPDDVF HCCHHHHCCCCCCCC	27.11	27050516
301	Phosphorylation	FSKEEMSSMPDDVFE CCHHHHCCCCCCCCC	32.78	20639409
308	Ubiquitination	SMPDDVFESPPLSAS CCCCCCCCCCCCCHH	63.28	21890473
308 (in isoform 2)	Ubiquitination	-	63.28	21890473
309	Phosphorylation	MPDDVFESPPLSASY CCCCCCCCCCCCHHH	21.98	20639409
313	Phosphorylation	VFESPPLSASYFRGI CCCCCCCCHHHHCCC	22.42	29116813
315	Phosphorylation	ESPPLSASYFRGIPH CCCCCCHHHHCCCCC	22.79	20639409
316	Phosphorylation	SPPLSASYFRGIPHS CCCCCHHHHCCCCCC	9.30	20639409
323	Phosphorylation	YFRGIPHSASPVSPD HHCCCCCCCCCCCCC	25.70	23401153
325	Phosphorylation	RGIPHSASPVSPDGV CCCCCCCCCCCCCCC	28.93	23927012
328	Phosphorylation	PHSASPVSPDGVQIP CCCCCCCCCCCCEEC	22.21	25159151
329	Ubiquitination	HSASPVSPDGVQIPL CCCCCCCCCCCEECH	42.48	33845483
337 (in isoform 1)	Ubiquitination	-	43.46	21890473
337	Ubiquitination	DGVQIPLKEYGRAPV CCCEECHHHHCCCCC	43.46	22817900
339	Phosphorylation	VQIPLKEYGRAPVPG CEECHHHHCCCCCCC	15.43	27642862
355	Phosphorylation	RRGKRIASKVKHFAF CCCHHHHHHCCEECC	35.29	24719451
356	Phosphorylation	RGKRIASKVKHFAFD CCHHHHHHCCEECCC	46.40	33259812
358	Ubiquitination	KRIASKVKHFAFDRK HHHHHHCCEECCCCC	36.42	33845483
381	Phosphorylation	VGNWLNRSYRRSISS HHHHHCHHHHHHHHH	23.17	24719451
385	Phosphorylation	LNRSYRRSISSTVQR HCHHHHHHHHHHHHH	20.16	30266825
387	Phosphorylation	RSYRRSISSTVQRQL HHHHHHHHHHHHHHH	22.62	29255136
388	Phosphorylation	SYRRSISSTVQRQLE HHHHHHHHHHHHHHH	30.81	30266825
389	Phosphorylation	YRRSISSTVQRQLES HHHHHHHHHHHHHHH	17.54	30266825
443	Ubiquitination	TQLVLRNKGVYESVK HHHHHCCCCHHHHHH	43.23	21963094
472	Ubiquitination	DLIHLGAKFSPCIRK EEEECCCCCCCCCCC	44.57	21963094
530	Phosphorylation	FVKWQDDTGPPMDKS HHHHCCCCCCCCCHH	60.93	-
544	Ubiquitination	SDLGQKRTSGAVCHQ HHHCCCCCCCCCCCC	38.25	21963094
573	Ubiquitination	IWPDDITKWPICTEQ CCCCCCCCCCCCCHH	51.84	21963094
576	Ubiquitination	DDITKWPICTEQARS CCCCCCCCCCHHHHH	4.39	21963094
605	Ubiquitination	RPCCIGTKGSCEITT CEEECCCCCCEEEEH	43.55	21963094
607	Phosphorylation	CCIGTKGSCEITTRE EECCCCCCEEEEHHH	15.33	-
700	Phosphorylation	IAIIFILSGITGNLA HHHHHHHHCCCCCHH	24.27	-
703	Phosphorylation	IFILSGITGNLASAI HHHHHCCCCCHHHHH	24.44	-
708	Phosphorylation	GITGNLASAIFLPYR CCCCCHHHHHHCCCC	25.68	-
819	Ubiquitination	FAGLFAALVLWLYIY HHHHHHHHHHHHHHC	2.67	21963094
848	Ubiquitination	FTSRFCEKYELDQVL CCHHHHHHCCHHHHC	45.25	21963094

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RHDF2_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RHDF2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RHDF2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UB2V1_HUMAN	UBE2V1	physical	29069608
UBE2N_HUMAN	UBE2N	physical	29069608
CHIP_HUMAN	STUB1	physical	29069608

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 148500: Tylosis with esophageal cancer (TOC)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90; SER-325 AND SER-328,AND MASS SPECTROMETRY.
PubMed: 18669648