dbPTM

RGRF1_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RGRF1_RAT
UniProt AC	P28818
Protein Name	Ras-specific guanine nucleotide-releasing factor 1
Gene Name	Rasgrf1
Organism	Rattus norvegicus (Rat).
Sequence Length	1244
Subcellular Localization
Protein Description	Promotes the exchange of Ras-bound GDP by GTP..
Protein Sequence	MQKAIRLNDGHVVSLGLLAQRDGTRKGYLSKRSSDNPKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGSICKRMPSPKRGTSSKESDKQHHYFTVNFSNDSQKSLELRTDDSKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKAEIANLIKDNERIQSNQLVAPEDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRVMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLIQVPMSEKGKINKGRLGSLSLKKEGERQCFLFSKHLIICTRGSGSKLHLTKNGVISLIDCTLLDDPENMDDDGKGQEVDHLDFKIWVEPKDSPPFTVILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTDAVVVLDKLISIYKKPITAIPARSLELLFSSSHNTKLLYGDAPKSPRASRKFSSPPPLAIGTSSPVRRRKLSLNIPIITGGKALELASLGCPSDGYTNIHSPISPFGKTTLDTSKLCVASSLTRTPEEIDMTTLEESSGFRKPTSDILKEESDDDQSDVDDTEVSPPTPKSFRNRITQEFPLFNYNSGIMMTCRDLMDSNRSPLSATSAFAIATAGANESPANKEIYRRMSLANTGYSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHSQDFETDDLLKYKVICFLEEVMHDPDLLPQERKAAANIMRTLTQEEITENHSMLDELLLMTEGVKTEPFENHSAMEIAEQLTLLDHLVFKSIPYEEFFGQGWMKADKNERTPYIMKTTRHFNHISNLIASEILRNEEVSARASTIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLFDKLQKLVSSDGRFKNLRETLRNCDPPCVPYLGMYLTDLAFLEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIEPQPKVTQYLVDETFVLDDESLYEASLRIEPKLPT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
71	Phosphorylation	GLYLLEGSICKRMPS CEEEEECCHHHCCCC	18.55	21382555
158	Ubiquitination	ETEKTVAKQLRQQLE HHHHHHHHHHHHHHH	45.59	-
204	Ubiquitination	EDEDSDIKKIKKVQS CCCCCCHHHHHHHHH	54.13	-
481	Phosphorylation	INKGRLGSLSLKKEG CCCCCCCCCCCCCCC	21.27	25403869
483	Phosphorylation	KGRLGSLSLKKEGER CCCCCCCCCCCCCCC	39.58	25403869
575	Phosphorylation	QEKAAWTSDIIQCVD CHHHHCHHCHHHHHH	18.99	21382555
611	Phosphorylation	QMIKSDASLYCDDVD HHHHCCHHEECCCCC	25.51	21382555
712	Phosphorylation	LYGDAPKSPRASRKF EECCCCCCCCCCCCC	20.68	25403869
720	Phosphorylation	PRASRKFSSPPPLAI CCCCCCCCCCCCCCC	44.99	22673903
721	Phosphorylation	RASRKFSSPPPLAIG CCCCCCCCCCCCCCC	44.55	16641100
729	Phosphorylation	PPPLAIGTSSPVRRR CCCCCCCCCCHHHCC	21.66	16641100
730	Phosphorylation	PPLAIGTSSPVRRRK CCCCCCCCCHHHCCC	27.68	16641100
731	Phosphorylation	PLAIGTSSPVRRRKL CCCCCCCCHHHCCCC	27.53	16921254
739	Phosphorylation	PVRRRKLSLNIPIIT HHHCCCCCCCCCEEE	23.54	25403869
760	Phosphorylation	LASLGCPSDGYTNIH HHHCCCCCCCCCCCC	47.98	21382555
763	Phosphorylation	LGCPSDGYTNIHSPI CCCCCCCCCCCCCCC	11.12	30322021
764	Phosphorylation	GCPSDGYTNIHSPIS CCCCCCCCCCCCCCC	32.97	30322021
781	Phosphorylation	GKTTLDTSKLCVASS CCCCCCHHHHHHHHC	24.25	21382555
812	Phosphorylation	SGFRKPTSDILKEES CCCCCCCHHHHHHCC	31.05	28551015
819	Phosphorylation	SDILKEESDDDQSDV HHHHHHCCCCCCCCC	47.64	25403869
824	Phosphorylation	EESDDDQSDVDDTEV HCCCCCCCCCCCCCC	46.74	25403869
829	Phosphorylation	DQSDVDDTEVSPPTP CCCCCCCCCCCCCCC	33.55	30322021
832	Phosphorylation	DVDDTEVSPPTPKSF CCCCCCCCCCCCHHH	20.94	25403869
835	Phosphorylation	DTEVSPPTPKSFRNR CCCCCCCCCHHHHCC	46.89	25403869
854	Phosphorylation	FPLFNYNSGIMMTCR ECCCCCCCCEEEEHH	21.24	21382555
894	Phosphorylation	SPANKEIYRRMSLAN CCCCHHHHHHHHHHH	8.02	26022182
898	Phosphorylation	KEIYRRMSLANTGYS HHHHHHHHHHHCCCC	23.63	25403869
979	Phosphorylation	ANIMRTLTQEEITEN HHHHHHCCHHHHHHC	33.06	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
71	S	Phosphorylation	Kinase	PLK1	P53350	PSP
71	S	Phosphorylation	Kinase	PLK2	Q9R012	Uniprot
575	S	Phosphorylation	Kinase	PLK1	P53350	PSP
575	S	Phosphorylation	Kinase	PLK2	Q9R012	Uniprot
611	S	Phosphorylation	Kinase	PLK1	P53350	PSP
611	S	Phosphorylation	Kinase	PLK2	Q9R012	Uniprot
731	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
760	S	Phosphorylation	Kinase	PLK1	P53350	PSP
760	S	Phosphorylation	Kinase	PLK2	Q9R012	Uniprot
781	S	Phosphorylation	Kinase	PLK1	P53350	PSP
781	S	Phosphorylation	Kinase	PLK2	Q9R012	Uniprot
854	S	Phosphorylation	Kinase	PLK1	P53350	PSP
854	S	Phosphorylation	Kinase	PLK2	Q9R012	Uniprot
898	S	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RGRF1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RGRF1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of RGRF1_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RGRF1_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Requirement for Plk2 in orchestrated ras and rap signaling,homeostatic structural plasticity, and memory."; Lee K.J., Lee Y., Rozeboom A., Lee J.Y., Udagawa N., Hoe H.S.,Pak D.T.; Neuron 69:957-973(2011). Cited for: PHOSPHORYLATION AT SER-71; SER-575; SER-611; SER-760; SER-781 ANDSER-854, UBIQUITINATION, AND MUTAGENESIS OF SER-71 AND SER-575.	21382555 [Abstract]