RGRF1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RGRF1_MOUSE
• UniProt AC: P27671
• Protein Name: Ras-specific guanine nucleotide-releasing factor 1
• Gene Name: Rasgrf1
• Organism: Mus musculus (Mouse).
• Sequence Length: 1262
• Protein Description: Promotes the exchange of Ras-bound GDP by GTP..
• Protein Sequence: MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEVTITNLIKDNDRIQSSNKAGSADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQMSLSEKSKSSRGRLGSLSTKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSLPFTVILVASTRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTNAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPATIPEKPGELSASRKHSSDVLKEESEDDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGILMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFENHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKAEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDISARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLRESLRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIDPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
71	Phosphorylation	GLYLLEGSICKRAPS CEEEEECCEECCCCC	18.55	-
118	Glutathionylation	RTEDAKDCDEWVAAI ECCCCCCHHHHHHHH	5.14	22833525
199	Phosphorylation	QSSNKAGSADDEDSD CCCCCCCCCCCCCCH	33.12	29899451
485	Phosphorylation	SSRGRLGSLSTKKEG CCCCCCCCCCCCCCC	24.60	23737553
487	Phosphorylation	RGRLGSLSTKKEGER CCCCCCCCCCCCCCC	39.62	23737553
488	Phosphorylation	GRLGSLSTKKEGERQ CCCCCCCCCCCCCCE	51.97	23737553
581	Phosphorylation	QEKAAWTSDIIQCVD CHHHHCCCCHHHHHH	18.99	-
617	Phosphorylation	QMIKSDASLYCDDVD HHHHCCHHEECCCCC	25.51	-
718	Phosphorylation	LYGDAPKSPRASRKF EECCCCCCCCCCCCC	20.68	29899451
726	Phosphorylation	PRASRKFSSPPPLAI CCCCCCCCCCCCEEE	44.99	22817900
727	Phosphorylation	RASRKFSSPPPLAIG CCCCCCCCCCCEEEC	44.55	22817900
735	Phosphorylation	PPPLAIGTSSPSRRR CCCEEECCCCHHHCC	21.66	21183079
736	Phosphorylation	PPLAIGTSSPSRRRK CCEEECCCCHHHCCC	34.73	23737553
737	Phosphorylation	PLAIGTSSPSRRRKL CEEECCCCHHHCCCC	27.08	25521595
739	Phosphorylation	AIGTSSPSRRRKLSL EECCCCHHHCCCCEE	40.13	23737553
745	Phosphorylation	PSRRRKLSLNIPIIT HHHCCCCEECCCEEE	23.54	11018028
765	Phosphorylation	ELASLGCSSDSYANI HHHHCCCCCCCCCCC	35.07	29899451
766	Phosphorylation	LASLGCSSDSYANIH HHHCCCCCCCCCCCC	34.35	-
769	Phosphorylation	LGCSSDSYANIHSPI CCCCCCCCCCCCCCC	14.52	29899451
774	Phosphorylation	DSYANIHSPISPFGK CCCCCCCCCCCCCCC	21.82	29899451
777	Phosphorylation	ANIHSPISPFGKTTL CCCCCCCCCCCCCCC	19.98	29899451
794	Phosphorylation	GKLCMASSLPKTPEE CCCCCCCCCCCCCHH	38.87	22871156
822	Phosphorylation	LSASRKHSSDVLKEE CCCCCCCCCCCCCCC	31.13	11018028
830	Phosphorylation	SDVLKEESEDDQNHS CCCCCCCCCCCCCCC	47.63	25521595
837	Phosphorylation	SEDDQNHSDEDNTEV CCCCCCCCCCCCCCC	50.69	25521595
842	Phosphorylation	NHSDEDNTEVSPVKS CCCCCCCCCCCCCCC	50.51	29899451
845	Phosphorylation	DEDNTEVSPVKSPPT CCCCCCCCCCCCCCC	20.11	25521595
849	Phosphorylation	TEVSPVKSPPTPKSF CCCCCCCCCCCCHHH	36.02	-
852	Phosphorylation	SPVKSPPTPKSFLNR CCCCCCCCCHHHHCC	46.89	-
916	Phosphorylation	KEVFRRMSLANTGFS HHHHHHHHHHHCCCC	23.63	22324799
953	Acetylation	VLRHWVTKHTQDFDT HHHHHHHHCCCCCCC	34.73	19855847

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
71	S	Phosphorylation	Kinase	PLK2	P53351	Uniprot
581	S	Phosphorylation	Kinase	PLK2	P53351	Uniprot
617	S	Phosphorylation	Kinase	PLK2	P53351	Uniprot
643	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
691	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
737	S	Phosphorylation	Kinase	CDK5	P49615	PSP
745	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
745	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
766	S	Phosphorylation	Kinase	PLK2	P53351	Uniprot
793	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
794	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
798	T	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
822	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
822	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
849	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
916	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
1123	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
1124	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
1146	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RGRF1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RGRF1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CTND1_MOUSE	Ctnnd1	physical	17993462

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations.";
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
Mol. Cell. Proteomics 6:283-293(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-727, AND MASSSPECTROMETRY.
PubMed: 17114649