RAB8A_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RAB8A_RAT
• UniProt AC: P35280
• Protein Name: Ras-related protein Rab-8A
• Gene Name: Rab8a
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 207
• Subcellular Localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus . Recycling endosome membrane . Cell projection, cilium . Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center,
• Protein Description: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis (By similarity). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. [PubMed: 21041651 Involved in autophagy (By similarity]
• Protein Sequence: MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSSHGVKITVEQQKRTSFFRCSLL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Acetylation	-----MAKTYDYLFK -----CCCCHHHHHH	46.44	22902405
4	Phosphorylation	----MAKTYDYLFKL ----CCCCHHHHHHH	16.46	30181290
5	Phosphorylation	---MAKTYDYLFKLL ---CCCCHHHHHHHH	11.24	30181290
7	Phosphorylation	-MAKTYDYLFKLLLI -CCCCHHHHHHHHHH	12.01	30181290
55	Acetylation	RTIELDGKRIKLQIW EEEEECCEEEEEEEE	51.09	22902405
58	Acetylation	ELDGKRIKLQIWDTA EECCEEEEEEEECCC	38.07	14656657
58	Ubiquitination	ELDGKRIKLQIWDTA EECCEEEEEEEECCC	38.07	-
72	Phosphorylation	AGQERFRTITTAYYR CCHHHHHHHHHHHHH	21.69	-
181	Phosphorylation	DKKLEGNSPQGSSHG HHHCCCCCCCCCCCC	29.47	30411139
185	Phosphorylation	EGNSPQGSSHGVKIT CCCCCCCCCCCEEEE	17.57	30411139
186	Phosphorylation	GNSPQGSSHGVKITV CCCCCCCCCCEEEEE	30.96	27097102
204	Methylation	KRTSFFRCSLL---- CCCCEEEEECC----	2.58	-
204	Geranylgeranylation	KRTSFFRCSLL---- CCCCEEEEECC----	2.58	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RAB8A_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
72	T	Phosphorylation	Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM and RAB GDP dissociation inhibitors GDI1 and GDI2.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RAB8A_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of RAB8A_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.