PUF60_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PUF60_MOUSE
• UniProt AC: Q3UEB3
• Protein Name: Poly(U)-binding-splicing factor PUF60
• Gene Name: Puf60
• Organism: Mus musculus (Mouse).
• Sequence Length: 564
• Subcellular Localization: Nucleus. Colocalizes partially with TROVE2..
• Protein Description: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity)..
• Protein Sequence: MATATIALQVNGQQGGGSEPAAAAAAAAAAVVAAGDKWKPPQGTESIKMENGQSTGTKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLATPGLVSPALTLAQPLGALPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQERFDNSDLSA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
37	Ubiquitination	AVVAAGDKWKPPQGT HHHHCCCCCCCCCCC	57.08	27667366
65	Phosphorylation	KLGLPPLTPEQQEAL CCCCCCCCHHHHHHH	30.47	26643407
78	Ubiquitination	ALQKAKKYAMEQSIK HHHHHHHHHHHHHHH	15.52	27667366
78	Phosphorylation	ALQKAKKYAMEQSIK HHHHHHHHHHHHHHH	15.52	26160508
83	Phosphorylation	KKYAMEQSIKSVLVK HHHHHHHHHHHHHHH	20.76	26160508
84	Ubiquitination	KYAMEQSIKSVLVKQ HHHHHHHHHHHHHHH	3.73	27667366
85	Ubiquitination	YAMEQSIKSVLVKQT HHHHHHHHHHHHHHH	39.50	22790023
85	Acetylation	YAMEQSIKSVLVKQT HHHHHHHHHHHHHHH	39.50	22826441
86	Phosphorylation	AMEQSIKSVLVKQTI HHHHHHHHHHHHHHH	21.00	26160508
109	Phosphorylation	NLQMAAVTMGFGDPL HHHHHHHHCCCCCCC	13.57	26745281
117	Phosphorylation	MGFGDPLSPLQSMAA CCCCCCCCHHHHHHH	29.02	26745281
121	Phosphorylation	DPLSPLQSMAAQRQR CCCCHHHHHHHHHHH	20.33	26745281
211	Phosphorylation	NIKVGRPSNIGQAQP CCCCCCCCCCCCCHH	39.45	26745281
243	Phosphorylation	ASVHQDLSDDDIKSV EEECCCCCHHHHHHH	47.48	21183079
248	Ubiquitination	DLSDDDIKSVFEAFG CCCHHHHHHHHHHHH	47.78	22790023
249	Phosphorylation	LSDDDIKSVFEAFGK CCHHHHHHHHHHHHC	32.16	24899341
256	Ubiquitination	SVFEAFGKIKSCTLA HHHHHHHCCEEEEEE	40.27	22790023
256	Acetylation	SVFEAFGKIKSCTLA HHHHHHHCCEEEEEE	40.27	22826441
272	Ubiquitination	DPTTGKHKGYGFIEY CCCCCCCCCCCEEEE	58.26	22790023
306	Ubiquitination	GQYLRVGKAVTPPMP CCEEEEECCCCCCCC	36.51	22790023
309	Phosphorylation	LRVGKAVTPPMPLLT EEEECCCCCCCCCCC	26.91	20469934
316	Phosphorylation	TPPMPLLTPATPGGL CCCCCCCCCCCCCCC	20.68	26745281
319	Phosphorylation	MPLLTPATPGGLPPA CCCCCCCCCCCCCHH	24.54	26745281
355	Phosphorylation	AVLGTLATPGLVSPA HHHHHHCCCCCCCHH	22.47	-
433	Phosphorylation	EEELFPESERPEMLS HHHHCCCCCCHHHCC	38.86	25777480
440	Phosphorylation	SERPEMLSEQEHMSI CCCHHHCCCCCCCCC	35.60	25777480
446	Phosphorylation	LSEQEHMSISGSSAR CCCCCCCCCCHHHHH	18.60	25777480
448	Phosphorylation	EQEHMSISGSSARHM CCCCCCCCHHHHHHH	25.89	25777480
450	Phosphorylation	EHMSISGSSARHMVM CCCCCCHHHHHHHHH	17.65	25777480
451	Phosphorylation	HMSISGSSARHMVMQ CCCCCHHHHHHHHHH	32.61	25777480
459	Acetylation	ARHMVMQKLLRKQES HHHHHHHHHHHCCHH	31.24	23806337
466	Phosphorylation	KLLRKQESTVMVLRN HHHHCCHHHHEEEEC	24.65	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PUF60_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PUF60_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PUF60_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of PUF60_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.