PSA_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PSA_MOUSE
• UniProt AC: Q11011
• Protein Name: Puromycin-sensitive aminopeptidase
• Gene Name: Npepps
• Organism: Mus musculus (Mouse).
• Sequence Length: 920
• Subcellular Localization: Cytoplasm, cytosol . Nucleus .
• Protein Description: Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins..
• Protein Sequence: MWLAAAVPSLARRLLLLGPPPPPLLLLLSRSSRRRRRLHSLGLAAMPEKRPFERLPAEVSPINYSLCLKPDLLDFTFEGKLEAAAQVRQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKIDFVGELNDKMKGFYRSRYTTPAGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLESASGKPIAAVMNTWTKQMGFPLIYVEAEQVEDDRVLKLSQKKFCASGPYGGEDCPQWMVPITISTSEDPNQAKLKILMDKPEMSVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDVFSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKEHVEGKQILSADLRSPVYLTVLKHGDGATLDIMLKLHKQADMQEEKNRIERVLGATLSPELIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELHNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDADSIHQYLLQRKTSPPSV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
29	Phosphorylation	PPLLLLLSRSSRRRR CCEEHHHCCCHHHHH	30.04	20531401
31	Phosphorylation	LLLLLSRSSRRRRRL EEHHHCCCHHHHHHH	25.48	20531401
32	Phosphorylation	LLLLSRSSRRRRRLH EHHHCCCHHHHHHHH	28.79	20531401
40	Phosphorylation	RRRRRLHSLGLAAMP HHHHHHHHCCCCCCC	28.82	-
67	S-nitrosylation	SPINYSLCLKPDLLD CCCCEEEECCHHHHC	3.64	21278135
67	S-nitrosocysteine	SPINYSLCLKPDLLD CCCCEEEECCHHHHC	3.64	-
80	Ubiquitination	LDFTFEGKLEAAAQV HCEEECCHHHHHHHH	35.64	-
119	Ubiquitination	GDEEIHATGFNYQNE CCCEEEECCCCCCCC	28.30	27667366
137	Phosphorylation	VTLSFPSTLQTGTGT EEEECCCEEECCCCE	24.11	28464351
156	Ubiquitination	FVGELNDKMKGFYRS EEEECCHHHCCEECC	41.25	22790023
174	Phosphorylation	TPAGEVRYAAVTQFE CCCHHEEEEEEEEEE	12.23	28464351
178	Phosphorylation	EVRYAAVTQFEATDA HEEEEEEEEEECCCH	23.86	-
183	Phosphorylation	AVTQFEATDARRAFP EEEEEECCCHHHHCC	24.00	-
209	Ubiquitination	DISLVVPKDRVALSN EEEEEECCCCEEECC	46.70	22790023
262	Ubiquitination	DFVETRSKDGVCVRV EEEEEECCCCEEEEE	56.03	22790023
275	Ubiquitination	RVYTPVGKAEQGKFA EEEECCCCHHCCCHH	49.32	22790023
330	Ubiquitination	GLVTYRETALLIDPK CCEEEEEEEEEECCC	17.38	27667366
465	Nitration	FKKGMNMYLTKFQQK HHHHHHHHHHHHHHC	13.25	16800626
465	Nitration	FKKGMNMYLTKFQQK HHHHHHHHHHHHHHC	13.25	16800626
465	Nitrated tyrosine	FKKGMNMYLTKFQQK HHHHHHHHHHHHHHC	13.25	-
468	Ubiquitination	GMNMYLTKFQQKNAA HHHHHHHHHHHCCCC	38.28	22790023
564	Ubiquitination	KLKILMDKPEMSVVL HEEEECCCCCCEEEE	28.98	22790023
575	Ubiquitination	SVVLKNVKPDQWVKL EEEEECCCHHCEEEE	52.88	22790023
621	Ubiquitination	VDRLGLQNDLFSLAR CCCCCCCCHHHHHHH	53.66	27667366
685	Phosphorylation	EFVKDVFSPIGERLG HHHHHHHCHHHHHHC	18.56	25521595
741	Phosphorylation	VEGKQILSADLRSPV HCCCEEECCCCCCCE	22.74	23984901
746	Phosphorylation	ILSADLRSPVYLTVL EECCCCCCCEEEEEE	26.47	25521595
749	Phosphorylation	ADLRSPVYLTVLKHG CCCCCCEEEEEEECC	10.47	25521595
751	Phosphorylation	LRSPVYLTVLKHGDG CCCCEEEEEEECCCC	13.20	23984901
777	Ubiquitination	QADMQEEKNRIERVL HHCHHHHHHHHHHHH	51.26	27667366
787	Phosphorylation	IERVLGATLSPELIQ HHHHHHCCCCHHHHH	26.43	22324799
789	Phosphorylation	RVLGATLSPELIQKV HHHHCCCCHHHHHHH	16.39	22324799
833	Acetylation	KAAWKFIKDNWEELH HHHHHHHHHCHHHHH	48.41	23236377
875	Phosphorylation	EVKAFFESHPAPSAE HHHHHHHCCCCCCHH	29.61	-
888	S-palmitoylation	AERTIQQCCENILLN HHHHHHHHHHHHHHH	1.48	28680068
905	Phosphorylation	WLKRDADSIHQYLLQ HHHCCHHHHHHHHHH	24.56	22817900
915	Phosphorylation	QYLLQRKTSPPSV-- HHHHHHCCCCCCC--	48.97	22324799
916	Phosphorylation	YLLQRKTSPPSV--- HHHHHCCCCCCC---	37.54	25521595
919	Phosphorylation	QRKTSPPSV------ HHCCCCCCC------	43.79	21183079

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PSA_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PSA_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PSA_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of PSA_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Nitration

"Endogenously nitrated proteins in mouse brain: links toneurodegenerative disease.";
Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H.,Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J.,Squier T.C., Bigelow D.J.;
Biochemistry 45:8009-8022(2006).
Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-465, AND MASS SPECTROMETRY.
PubMed: 16800626