PPM1A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PPM1A_MOUSE
• UniProt AC: P49443
• Protein Name: Protein phosphatase 1A
• Gene Name: Ppm1a
• Organism: Mus musculus (Mouse).
• Sequence Length: 382
• Subcellular Localization: Nucleus . Cytoplasm, cytosol . Membrane ; Lipid-anchor . Weakly associates at the membrane and N-myristoylation mediates the membrane localization.
• Protein Description: Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity). Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB..
• Protein Sequence: MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLETWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFRGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPSAPKVSAEAVKKEAELDKYLESRVEEIIKKQVEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSASTDDMW

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Myristoylation	------MGAFLDKPK ------CCCCCCCHH	25.65	23088624
88	Phosphorylation	NNQDFRGSAGAPSVE CCCCCCCCCCCCCHH	21.19	28059163
93	Phosphorylation	RGSAGAPSVENVKNG CCCCCCCCHHHHHCC	41.34	28059163
98	Ubiquitination	APSVENVKNGIRTGF CCCHHHHHCCCCCCC	62.12	22790023
135	Phosphorylation	TAVGVLISPQHTYFI CEEEEEECCCCEEEE	17.85	-
181	Phosphorylation	RIQNAGGSVMIQRVN HHHHCCCCEEEEEEC	13.95	29514104
190	Phosphorylation	MIQRVNGSLAVSRAL EEEEECCCHHHHHHH	14.14	22324799
194	Phosphorylation	VNGSLAVSRALGDFD ECCCHHHHHHHCCCC	13.26	22324799
216	Phosphorylation	GPTEQLVSPEPEVHD CCHHHCCCCCCCCCC	31.94	20415495
277	Phosphorylation	EVVDTCLYKGSRDNM HHHHHHHHCCCCCCE	18.56	21454597
280	Phosphorylation	DTCLYKGSRDNMSVI HHHHHCCCCCCEEEE	32.10	21454597
310	Acetylation	KKEAELDKYLESRVE HHHHHHHHHHHHHHH	65.44	23954790
322	Ubiquitination	RVEEIIKKQVEGVPD HHHHHHHHHHCCCCH	49.52	22790023
368	Phosphorylation	VYNRLNPYKNDDTDS HHHHHCCCCCCCCCC	22.73	29899451
373	Phosphorylation	NPYKNDDTDSASTDD CCCCCCCCCCCCCCC	34.42	23737553
375	Phosphorylation	YKNDDTDSASTDDMW CCCCCCCCCCCCCCC	26.64	23684622
377	Phosphorylation	NDDTDSASTDDMW-- CCCCCCCCCCCCC--	36.00	23088624
378	Phosphorylation	DDTDSASTDDMW--- CCCCCCCCCCCC---	35.42	23737553
381	Oxidation	DSASTDDMW------ CCCCCCCCC------	5.21	17242355

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
375	S	Phosphorylation	Kinase	CSNK2A1	Q60737	GPS
377	S	Phosphorylation	Kinase	CSNK2A1	Q60737	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PPM1A_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PPM1A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377, AND MASSSPECTROMETRY.
PubMed: 17242355