PLEK_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PLEK_HUMAN
• UniProt AC: P08567
• Protein Name: Pleckstrin
• Gene Name: PLEK
• Organism: Homo sapiens (Human).
• Sequence Length: 350
• Protein Description: Major protein kinase C substrate of platelets..
• Protein Sequence: MEPKRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFGKRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIKCIEGGQKFARKSTRRSIRLPETIDLGALYLSMKDTEKGIKELNLEKDKKIFNHCFTGNCVIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFLDNPDAFYYFPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSNGRKSEEENLFEIITADEVHYFLQAATPKERTEWIRAIQMASRTGK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	PKRIREGYLVKKGSV CCCCCEEEEEECCCC	11.99	28060719
16	Phosphorylation	GYLVKKGSVFNTWKP EEEEECCCCCCCCCE	32.20	28270605
51	Acetylation	PKGMIPLKGSTLTSP CCCCEECCCCCCCCC	45.52	19813131
53	Phosphorylation	GMIPLKGSTLTSPCQ CCEECCCCCCCCCCH	20.75	28060719
54	Phosphorylation	MIPLKGSTLTSPCQD CEECCCCCCCCCCHH	42.60	28060719
56	Phosphorylation	PLKGSTLTSPCQDFG ECCCCCCCCCCHHHC	30.61	28060719
57	Phosphorylation	LKGSTLTSPCQDFGK CCCCCCCCCCHHHCC	27.13	28060719
64	Acetylation	SPCQDFGKRMFVFKI CCCHHHCCCEEEEEE	40.46	19608861
101	Ubiquitination	RDIKKAIKCIEGGQK HHHHHHHHHHHHCHH	33.74	25015289
108	Acetylation	KCIEGGQKFARKSTR HHHHHCHHHHCHHCC	44.24	25953088
108	Ubiquitination	KCIEGGQKFARKSTR HHHHHCHHHHCHHCC	44.24	25015289
113	Phosphorylation	GQKFARKSTRRSIRL CHHHHCHHCCCCCCC	22.92	8615792
114	Phosphorylation	QKFARKSTRRSIRLP HHHHCHHCCCCCCCC	32.89	22817900
117	Phosphorylation	ARKSTRRSIRLPETI HCHHCCCCCCCCCEE	14.58	20058876
123	Phosphorylation	RSIRLPETIDLGALY CCCCCCCEEECCCHH	20.18	28450419
130	Phosphorylation	TIDLGALYLSMKDTE EEECCCHHHHHHHHH	9.01	28857561
132	Phosphorylation	DLGALYLSMKDTEKG ECCCHHHHHHHHHHH	15.38	28857561
136	Phosphorylation	LYLSMKDTEKGIKEL HHHHHHHHHHHHHHC	33.79	-
183	Phosphorylation	EGLMIASSLLNEGYL HHHHHHHHHHHCCCC	28.05	17924679
197	Phosphorylation	LQPAGDMSKSAVDGT CCCCCCCCCHHCCCC	28.35	17924679
217	Phosphorylation	LDNPDAFYYFPDSGF CCCCCCEEECCCCCC	13.10	26657352
218	Phosphorylation	DNPDAFYYFPDSGFF CCCCCEEECCCCCCC	11.37	28060719
222	Phosphorylation	AFYYFPDSGFFCEEN CEEECCCCCCCCCCC	37.42	28060719
230	Phosphorylation	GFFCEENSSDDDVIL CCCCCCCCCCCCEEE	38.12	28060719
231	Phosphorylation	FFCEENSSDDDVILK CCCCCCCCCCCEEEC	57.55	26657352
274	Phosphorylation	ILREDPAYLHYYDPA EECCCCCEEEECCCC	10.21	28270605
277	Phosphorylation	EDPAYLHYYDPAGAE CCCCEEEECCCCCCC	13.63	28270605
278	Phosphorylation	DPAYLHYYDPAGAED CCCEEEECCCCCCCC	12.52	22817900
299	Phosphorylation	LRGCVVTSVESNSNG EECEEEEEEECCCCC	16.57	28348404
302	Phosphorylation	CVVTSVESNSNGRKS EEEEEEECCCCCCCC	43.32	30108239
304	Phosphorylation	VTSVESNSNGRKSEE EEEEECCCCCCCCCH	50.36	30108239
340	Ubiquitination	KERTEWIRAIQMASR HHHHHHHHHHHHHHH	27.06	21890473

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
113	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
113	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
113	S	Phosphorylation	Kinase	PKC	-	Uniprot
113	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
114	T	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
114	T	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
117	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
117	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
117	S	Phosphorylation	Kinase	PKC	-	Uniprot
117	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PLEK_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
230	Phosphorylation	229 (1)	N ⇒ H	rs34338164	Lymphocyte counts Plateletcrit	27863252
231	Phosphorylation	229 (2)	N ⇒ H	rs34338164	Lymphocyte counts Plateletcrit	27863252

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
WDR75_HUMAN	WDR75	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-64, AND MASS SPECTROMETRY.
PubMed: 19608861

Phosphorylation

"Phosphorylation analysis of primary human T lymphocytes usingsequential IMAC and titanium oxide enrichment.";
Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.;
J. Proteome Res. 7:5167-5176(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, AND MASSSPECTROMETRY.
PubMed: 19367720

"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.";
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.;
J. Proteome Res. 6:4150-4162(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183 AND SER-197, ANDMASS SPECTROMETRY.
PubMed: 17924679

"Phosphorylation of human pleckstrin on Ser-113 and Ser-117 by proteinkinase C.";
Craig K.L., Harley C.B.;
Biochem. J. 314:937-942(1996).
Cited for: PHOSPHORYLATION AT SER-113 AND SER-117.
PubMed: 8615792