PKHF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PKHF1_HUMAN
• UniProt AC: Q96S99
• Protein Name: Pleckstrin homology domain-containing family F member 1
• Gene Name: PLEKHF1
• Organism: Homo sapiens (Human).
• Sequence Length: 279
• Subcellular Localization: Nucleus . Cytoplasm, perinuclear region . Lysosome . Translocates to lysosome during apoptosis.
• Protein Description: May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8..
• Protein Sequence: MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8	Phosphorylation	MVDHLANTEINSQRI CCCCCCCCCCHHHHH	32.41	25002506
12	Phosphorylation	LANTEINSQRIAAVE CCCCCCHHHHHHHHH	26.88	25002506
44	Malonylation	LGEGVLTKECRKKAK ECCCCCCHHHHHHCC	51.13	26320211
44	Acetylation	LGEGVLTKECRKKAK ECCCCCCHHHHHHCC	51.13	23749302
44	Ubiquitination	LGEGVLTKECRKKAK ECCCCCCHHHHHHCC	51.13	19608861
97	Ubiquitination	LPETLQAKNRWMIKT CHHHHHHCCCEEEHH	33.80	-
108	Phosphorylation	MIKTAKKSFVVSAAS EEHHCHHHEEEECCC	23.67	21955146
112	Phosphorylation	AKKSFVVSAASATER CHHHEEEECCCCHHH	16.89	21955146
115	Phosphorylation	SFVVSAASATERQEW HEEEECCCCHHHHHH	35.13	21955146
137	Phosphorylation	VRRQLRATGRPPSTE HHHHHHHHCCCCCCC	27.56	26437602
142	Phosphorylation	RATGRPPSTEHAAPW HHHCCCCCCCCCCCC	49.46	28348404
143	Phosphorylation	ATGRPPSTEHAAPWI HHCCCCCCCCCCCCC	37.25	28348404
197	Phosphorylation	RFLLPRLSPKPVRVC CCCCCCCCCCCEEHH	31.96	26434776
205	Phosphorylation	PKPVRVCSLCYRELA CCCEEHHHHHHHHHH	20.72	22617229
227	Phosphorylation	AEEQGAGSPGQPAHL HHHCCCCCCCCCCCC	25.89	23401153
242	Phosphorylation	ARPICGASSGDDDDS CCCCCCCCCCCCCCC	21.82	23663014
243	Phosphorylation	RPICGASSGDDDDSD CCCCCCCCCCCCCCC	45.79	23663014
249	Phosphorylation	SSGDDDDSDEDKEGS CCCCCCCCCCCCCCC	50.31	23663014
256	Phosphorylation	SDEDKEGSRDGDWPS CCCCCCCCCCCCCCC	28.43	25850435

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PKHF1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PKHF1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PKHF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LMBL3_HUMAN	L3MBTL3	physical	25416956

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-44, AND MASS SPECTROMETRY.
PubMed: 19608861