PKHA8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PKHA8_HUMAN
• UniProt AC: Q96JA3
• Protein Name: Pleckstrin homology domain-containing family A member 8
• Gene Name: PLEKHA8
• Organism: Homo sapiens (Human).
• Sequence Length: 519
• Subcellular Localization: Golgi apparatus, trans-Golgi network membrane . Membrane; Peripheral membrane protein . Binds through its PH domain to PtdIns(4)P and ARF1, and subsequently localizes to TGN exit sites.
• Protein Description: Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation..
• Protein Sequence: MEGVLYKWTNYLSGWQPRWFLLCGGILSYYDSPEDAWKGCKGSIQMAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTTGVSNSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNSLERQMELSTCENGSLNMEINGEEEILMKNKNSLYLKSAEIDCSISSEENTDDNITVQGEIRKEDGMENLKNHDNNLTQSGSDSSCSPECLWEEGKEVIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFALALRAAPSYEDFVAALTVKEGDHQKEAFSIGMQRDLSLYLPAMEKQLAILDTLYEVHGLESDEVV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
77	Phosphorylation	YFYLKARSVAERQRW EEEEEEHHHHHHHHH	30.45	23403867
90	Phosphorylation	RWLVALGSAKACLTD HHHHHHHHHHHHHCC	27.70	29759185
96	Phosphorylation	GSAKACLTDSRTQKE HHHHHHHCCCCHHHH	31.64	29759185
98	Phosphorylation	AKACLTDSRTQKEKE HHHHHCCCCHHHHHH	31.80	29759185
100	Phosphorylation	ACLTDSRTQKEKEFA HHHCCCCHHHHHHHH	48.20	29759185
115	Phosphorylation	ENTENLKTKMSELRL HHHHCHHHHHHHHHH	34.84	24719451
123	Phosphorylation	KMSELRLYCDLLVQQ HHHHHHHHHHHHHHH	4.22	24719451
138	Phosphorylation	VDKTKEVTTTGVSNS CCCCCEEECCCCCCC	21.52	19690332
139	Phosphorylation	DKTKEVTTTGVSNSE CCCCEEECCCCCCCC	27.08	-
145	Phosphorylation	TTTGVSNSEEGIDVG ECCCCCCCCCCCCHH	29.53	-
153	Phosphorylation	EEGIDVGTLLKSTCN CCCCCHHHHHHHHHH	28.85	19690332
156	Ubiquitination	IDVGTLLKSTCNTFL CCHHHHHHHHHHHHH	47.00	-
165	Phosphorylation	TCNTFLKTLEECMQI HHHHHHHHHHHHHHH	41.36	-
178	Phosphorylation	QIANAAFTSELLYRT HHHHHHHHHHHHHCC	19.30	27422710
179	Phosphorylation	IANAAFTSELLYRTP HHHHHHHHHHHHCCC	21.57	28348404
183	Phosphorylation	AFTSELLYRTPPGSP HHHHHHHHCCCCCCC	25.68	26074081
185	Phosphorylation	TSELLYRTPPGSPQL HHHHHHCCCCCCCCH	21.93	23663014
189	Phosphorylation	LYRTPPGSPQLAMLK HHCCCCCCCCHHHHH	18.42	23663014
196	Acetylation	SPQLAMLKSSKMKHP CCCHHHHHCCCCCCC	39.33	-
201	Ubiquitination	MLKSSKMKHPIIPIH HHHCCCCCCCCCCCC	50.38	-
210	Phosphorylation	PIIPIHNSLERQMEL CCCCCCCCHHHHHHH	19.90	24719451
242	Phosphorylation	ILMKNKNSLYLKSAE EEECCCCCEEEEECE	21.72	-
293	Phosphorylation	LTQSGSDSSCSPECL CCCCCCCCCCCHHHH	34.63	22468782
296	Phosphorylation	SGSDSSCSPECLWEE CCCCCCCCHHHHHHC	26.19	22468782
358	Ubiquitination	PTVFAPVKMDLVGNI CCEEEEEECCCCCCH	26.15	-
380	Phosphorylation	ITNKEEFTTLQKIVL CCCHHHCHHHHHHHH	29.85	19664994
381	Phosphorylation	TNKEEFTTLQKIVLH CCHHHCHHHHHHHHH	32.29	19664994
384	Ubiquitination	EEFTTLQKIVLHEVE HHCHHHHHHHHHHCC	37.58	-
416	Ubiquitination	KRGLKFLKGFLTEVK HHHHHHHHHHHHHHH	51.23	2189047
416	Ubiquitination	KRGLKFLKGFLTEVK HHHHHHHHHHHHHHH	51.23	21890473
416 (in isoform 1)	Ubiquitination	-	51.23	21890473
416 (in isoform 2)	Ubiquitination	-	51.23	21890473
416 (in isoform 3)	Ubiquitination	-	51.23	21890473
420	Phosphorylation	KFLKGFLTEVKNGEK HHHHHHHHHHHCCHH	36.15	20230923
427	Ubiquitination	TEVKNGEKDIQTALN HHHHCCHHHHHHHHH	62.62	-
431	Phosphorylation	NGEKDIQTALNNAYG CCHHHHHHHHHHHHH	33.18	-
437	Phosphorylation	QTALNNAYGKTLRQH HHHHHHHHHHHHHHH	22.68	-
462	Phosphorylation	LALRAAPSYEDFVAA HHHHHCCCHHHHEEE	36.11	27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PKHA8_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PKHA8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PKHA8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ARF1_HUMAN	ARF1	physical	15107860

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.