PIWL1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PIWL1_MOUSE
• UniProt AC: Q9JMB7
• Protein Name: Piwi-like protein 1 {ECO:0000305}
• Gene Name: Piwil1 {ECO:0000312|MGI:MGI:1928897}
• Organism: Mus musculus (Mouse).
• Sequence Length: 862
• Subcellular Localization: Cytoplasm . Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Also present in chromatoid body.
• Protein Description: Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. [PubMed: 11578866]
• Protein Sequence: MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
14	Dimethylation	ARARGRARGQETVQH HHHHHHHCCCHHHHH	46.06	-
14	Methylation	ARARGRARGQETVQH HHHHHHHCCCHHHHH	46.06	19584108
49	Methylation	TEGDLVGRGRQRGMV CCCCCCCCCCCCCEE	29.64	19584108
53	Symmetric dimethylarginine	LVGRGRQRGMVVGAT CCCCCCCCCEEEECC	33.54	-
53	Methylation	LVGRGRQRGMVVGAT CCCCCCCCCEEEECC	33.54	19918066
330	Ubiquitination	IDWDQNPKSTFKKAD CCCCCCCCCCCCCCC	70.45	23328397
371	Methylation	VSQPKRRRGPGGTLP ECCCCCCCCCCCCCC	60.74	19584108
405	Ubiquitination	RNDFNVMKDLAVHTR HCCCHHHHHHHHHHC	45.36	-
431	Ubiquitination	RLIDYIHKDDNVQRE HHHHHHCCCCCHHHH	58.16	-
462	Ubiquitination	GRILQSEKIHQGGKT CEEECCCEEECCCCE	51.22	-
481	Ubiquitination	PQFADWSKETRGAPL HHHCCCCCCCCCCCE	59.87	-
574	Ubiquitination	DKYDAIKKYLCTDCP CHHHHHHHHCCCCCC	37.02	-
651	Phosphorylation	SIAGFVASINEGMTR CCCEEEEECCCCHHH	22.20	-
661	Phosphorylation	EGMTRWFSRCVFQDR CCHHHHHHHHEECCC	20.15	30482847
737	Phosphorylation	RGYNPRLTVIVVKKR CCCCCCEEEEEEECC	14.96	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PIWL1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PIWL1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PIWL1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of PIWL1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Methylation

"Proteomic analysis of murine Piwi proteins reveals a role forarginine methylation in specifying interaction with Tudor familymembers.";
Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S.,Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.;
Genes Dev. 23:1749-1762(2009).
Cited for: METHYLATION AT ARG-14; ARG-49 AND ARG-371, SUBCELLULAR LOCATION, ANDINTERACTION WITH TDRD1; TDRKH; TDRD6; PRMT5 AND WDR77.
PubMed: 19584108