dbPTM

PIPNB_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PIPNB_MOUSE
UniProt AC	P53811
Protein Name	Phosphatidylinositol transfer protein beta isoform
Gene Name	Pitpnb
Organism	Mus musculus (Mouse).
Sequence Length	271
Subcellular Localization	Cytoplasm. Golgi apparatus.
Protein Description	Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes..
Protein Sequence	MVLIKEFRVVLPCSVQEYQVGQLYSVAEASKNETGGGEGIEVLKNEPYENDGEKGQYTHKIYHLKSKVPAFVRMIAPEGSLVFHEKAWNAYPYCRTIVTNEYMKDDFFIKIETWHKPDLGTLENVHGLDPNTWKTVEIVHIDIADRSQVEPADYKADEDPALFHSVKTKRGPLGPNWKKELANTPDCPRMCAYKLVTIKFKWWGLQSKVENFIQKQEKRIFTNLHRQLFCWIDKWIDLTMEDIRRMEDETQKELETMRKKGSVRGTSAADA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Ubiquitination	---MVLIKEFRVVLP ---CCEEEEEEEEEE	46.79	-
5	Acetylation	---MVLIKEFRVVLP ---CCEEEEEEEEEE	46.79	22826441
13	S-palmitoylation	EFRVVLPCSVQEYQV EEEEEEEECCEEEEC	5.64	28526873
44	Acetylation	GEGIEVLKNEPYEND CCCEEEECCCCCCCC	65.52	23954790
54	Acetylation	PYENDGEKGQYTHKI CCCCCCCCCCCCHHH	57.77	23954790
54	Ubiquitination	PYENDGEKGQYTHKI CCCCCCCCCCCCHHH	57.77	-
65	Acetylation	THKIYHLKSKVPAFV CHHHEEHHHCCCEEE	34.61	22826441
86	Ubiquitination	GSLVFHEKAWNAYPY CCEEEECCCHHHCCC	50.56	-
94	S-nitrosocysteine	AWNAYPYCRTIVTNE CHHHCCCEEEEECCC	2.40	-
94	S-palmitoylation	AWNAYPYCRTIVTNE CHHHCCCEEEEECCC	2.40	28526873
94	S-nitrosylation	AWNAYPYCRTIVTNE CHHHCCCEEEEECCC	2.40	21278135
165	Phosphorylation	EDPALFHSVKTKRGP CCCHHHCCCCCCCCC	20.27	11953429
179	Malonylation	PLGPNWKKELANTPD CCCCCHHHHHCCCCC	49.96	26320211
187	S-palmitoylation	ELANTPDCPRMCAYK HHCCCCCCHHHCHHE	2.13	28526873
187	S-nitrosylation	ELANTPDCPRMCAYK HHCCCCCCHHHCHHE	2.13	21278135
187	S-nitrosocysteine	ELANTPDCPRMCAYK HHCCCCCCHHHCHHE	2.13	-
194	Acetylation	CPRMCAYKLVTIKFK CHHHCHHEEEEEEHH	20.25	22826441
199	Acetylation	AYKLVTIKFKWWGLQ HHEEEEEEHHHHCCH	31.37	22826441
201	Acetylation	KLVTIKFKWWGLQSK EEEEEEHHHHCCHHH	36.71	22826441
201	Ubiquitination	KLVTIKFKWWGLQSK EEEEEEHHHHCCHHH	36.71	-
215	Malonylation	KVENFIQKQEKRIFT HHHHHHHHHHHHHHH	56.46	26320211
215	Acetylation	KVENFIQKQEKRIFT HHHHHHHHHHHHHHH	56.46	22826441
230	S-nitrosylation	NLHRQLFCWIDKWID HHHHHHHHHHHHHHC	4.18	21278135
230	S-nitrosocysteine	NLHRQLFCWIDKWID HHHHHHHHHHHHHHC	4.18	-
230	S-palmitoylation	NLHRQLFCWIDKWID HHHHHHHHHHHHHHC	4.18	28526873
262	Phosphorylation	ETMRKKGSVRGTSAA HHHHHHCCCCCCCCC	19.95	26824392
266	Phosphorylation	KKGSVRGTSAADA-- HHCCCCCCCCCCC--	12.48	18846507
267	Phosphorylation	KGSVRGTSAADA--- HCCCCCCCCCCC---	25.04	18846507

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
165	S	Phosphorylation	Kinase	PRKCA	P20444	GPS
165	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
262	S	Phosphorylation	Kinase	PRKCA	P20444	GPS
262	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
262	S	Phosphorylation	Kinase	PKC	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
262	S	Phosphorylation		11953429
Constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity but is required for Golgi targeting.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PIPNB_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of PIPNB_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PIPNB_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"The Golgi localization of phosphatidylinositol transfer protein betarequires the protein kinase C-dependent phosphorylation of serine 262and is essential for maintaining plasma membrane sphingomyelinlevels."; van Tiel C.M., Westerman J., Paasman M.A., Hoebens M.M., Wirtz K.W.,Snoek G.T.; J. Biol. Chem. 277:22447-22452(2002). Cited for: PHOSPHORYLATION AT SER-262, MUTAGENESIS OF SER-165 AND SER-262, ANDMASS SPECTROMETRY.	11953429 [Abstract]

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