PFKAL_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PFKAL_RAT
• UniProt AC: P30835
• Protein Name: ATP-dependent 6-phosphofructokinase, liver type {ECO:0000255|HAMAP-Rule:MF_03184}
• Gene Name: Pfkl
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 780
• Subcellular Localization: Cytoplasm .
• Protein Description: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity)..
• Protein Sequence: MATVDLEKLRMSGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKPANWLSVSNIIQLGGTIIGSARCKAFTTREGRLAAAYNLLQHGITNLCVIGGDGSLTGANIFRNEWGSLLEELVKEGKISESTAQNYAHLSIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRHGKPISSSYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRVLSSKMGMEAVMALLEATPDTPACVVSLSGNQSVRLPLMECVQVTKDVQKAMDEKRFDEAIQLRGRSFENNWKIYKLLAHQKVSKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISEGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKPHLEAIVENLRTYNIHALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFLYNLYSSEGRGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWMSEKLRDVYRKGRVFANAPDSACVIGLRKKVVAFSSVTELKKETDFEHRMPREQWWLNLRLMLKMLAHYRISMADYVSGELEHVTRRTLSIDKGF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MATVDLEKL ------CCCCCHHHH		-
8	Ubiquitination	MATVDLEKLRMSGAG CCCCCHHHHHHCCCC		-
12	Phosphorylation	DLEKLRMSGAGKAIG CHHHHHHCCCCCEEE		26022182
22	Phosphorylation	GKAIGVLTSGGDAQG CCEEEEEECCCCHHH		26022182
23	Phosphorylation	KAIGVLTSGGDAQGM CEEEEEECCCCHHHH		26022182
304	Phosphorylation	GHVQRGGTPSAFDRV ECHHHCCCCHHHHHH		25403869
306	Phosphorylation	VQRGGTPSAFDRVLS HHHCCCCHHHHHHHH		22673903
360	Acetylation	QVTKDVQKAMDEKRF HHHHHHHHHHHHCCH		22902405
365	Acetylation	VQKAMDEKRFDEAIQ HHHHHHHCCHHHHHH		22902405
377	Phosphorylation	AIQLRGRSFENNWKI HHHHCCCCCCCCHHH		23984901
383	Acetylation	RSFENNWKIYKLLAH CCCCCCHHHHHHHHC		22902405
386	Acetylation	ENNWKIYKLLAHQKV CCCHHHHHHHHCCCC		22902405
474	Acetylation	GTKRTLPKPHLEAIV CCCCCCCCHHHHHHH		22902405
529	O-linked_Glycosylation	CVIPATISNNVPGTD EEEECHHCCCCCCCC		-
640	Phosphorylation	HYTTEFLYNLYSSEG CCCHHHHHHHHCCCC		-
677	Acetylation	FDRNYGTKLGVKAML CCCCHHHHHHHHHHH		22902405
726	Acetylation	FSSVTELKKETDFEH EECHHHHHHHCCCCC		22902405
773	Phosphorylation	LEHVTRRTLSIDKGF CEECEEEEEECCCCC		2539199
775	Phosphorylation	HVTRRTLSIDKGF-- ECEEEEEECCCCC--		23712012

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
773	T	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
529	S	Oxidation	GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PFKAL_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of PFKAL_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.