PFKAL_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PFKAL_MOUSE
• UniProt AC: P12382
• Protein Name: ATP-dependent 6-phosphofructokinase, liver type {ECO:0000255|HAMAP-Rule:MF_03184}
• Gene Name: Pfkl {ECO:0000312|MGI:MGI:97547}
• Organism: Mus musculus (Mouse).
• Sequence Length: 780
• Subcellular Localization: Cytoplasm .
• Protein Description: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway. [PubMed: 26194095]
• Protein Sequence: MATVDLEKLRMSGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKPANWLSVSNIIQLGGTIIGSARCKAFTTREGRLAAAYNLLQHGITNLCVIGGDGSLTGANIFRNEWGSLLEELVKEGKISESTAQNYAHLTIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRHGKPISSSYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVSLSGNQSVRLPLMECVQVTKDVQKAMDEERFDEAIQLRGRSFENNWKIYKLLAHQKVSKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISEGHTVYIVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKPHLEAIVENLRTYNIHALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFLYNLYSSEGRGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWVSEKLRDVYRKGRVFANAPDSACVIGLRKKVVAFSPVTELKKETDFEHRMPREQWWLNLRLMLKMLAHYRISMADYVSGELEHVTRRTLSIDKGF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MATVDLEKL ------CCCCCHHHH		-
8	Ubiquitination	MATVDLEKLRMSGAG CCCCCHHHHHHCCCC		22790023
72	Phosphorylation	IKPANWLSVSNIIQL CCCCCCEEHHHEEEC		23984901
74	Phosphorylation	PANWLSVSNIIQLGG CCCCEEHHHEEECCC		23984901
82	Phosphorylation	NIIQLGGTIIGSARC HEEECCCEEEEECCC		23984901
86	Phosphorylation	LGGTIIGSARCKAFT CCCEEEEECCCCEEE		23984901
301	Ubiquitination	TVLGHVQRGGTPSAF EEEECHHHCCCHHHH		27667366
304	Phosphorylation	GHVQRGGTPSAFDRI ECHHHCCCHHHHHHH		24899341
306	Phosphorylation	VQRGGTPSAFDRILS HHHCCCHHHHHHHHH		23737553
351	S-nitrosocysteine	VRLPLMECVQVTKDV EEEEHHHHHHHHHHH		-
362	Ubiquitination	TKDVQKAMDEERFDE HHHHHHHHCHHHHHH		27667366
377	Phosphorylation	AIQLRGRSFENNWKI HHHHHCCCCCCCHHH		22817900
394	Phosphorylation	LLAHQKVSKEKSNFS HHHCCCCCCCCCCCE		25338131
398	Phosphorylation	QKVSKEKSNFSLAIL CCCCCCCCCCEEEEE		21454597
401	Phosphorylation	SKEKSNFSLAILNVG CCCCCCCEEEEEECC		21454597
469	Malonylation	GGSMLGTKRTLPKPH CCCCCCCCCCCCCHH		26320211
529	O-linked_Glycosylation	CVIPATISNNVPGTD EEEECHHCCCCCCCC		-
575	Phosphorylation	IVETMGGYCGYLATV EEEECCCCHHHHHHH		22817900
616	Ubiquitination	EHMTEKMKTDIQRGL HHHHHHHHHHHHHCC		27667366
629	Acetylation	GLVLRNEKCHEHYTT CCCCCCCCCCCCCCH		22826441
640	Phosphorylation	HYTTEFLYNLYSSEG CCCHHHHHHHHCCCC		22817900
677	Ubiquitination	FDRNYGTKLGVKAML CCCCHHHHHHHHHHH		27667366
677	Acetylation	FDRNYGTKLGVKAML CCCCHHHHHHHHHHH		23806337
708	S-nitrosocysteine	ANAPDSACVIGLRKK CCCCCCEEEEECCCE		-
726	Acetylation	FSPVTELKKETDFEH ECCCHHHCCCCCCCC		23954790
726	Malonylation	FSPVTELKKETDFEH ECCCHHHCCCCCCCC		26320211
761	Phosphorylation	YRISMADYVSGELEH HHHHHHHHHHCCCEE		26026062
773	Phosphorylation	LEHVTRRTLSIDKGF CEECEEEEEECCCCC		27742792
775	Phosphorylation	HVTRRTLSIDKGF-- ECEEEEEECCCCC--		18388127

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PFKAL_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
529	S	Oxidation	GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PFKAL_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of PFKAL_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.