dbPTM

PDE8A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PDE8A_MOUSE
UniProt AC	O88502
Protein Name	High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Gene Name	Pde8a
Organism	Mus musculus (Mouse).
Sequence Length	823
Subcellular Localization
Protein Description	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling. Protects against cell death induced by hydrogen peroxide and staurosporine..
Protein Sequence	MGCAPSIHTSENRTFSHSDGEDEDVDVDVPGPAPRSIQRWSTAPGLVEPQPRDNGASKVSVADVQFGPMRFHQDQLQVLLVFTKEDSQCNGFHRACEKAGFKCTVTKEVQTVLTCFQDKLHDIIIIDHRYPRQMDAETLCRSIRSSKFSENTVIVGVVRRVDKEESSLMPFLAAGFTRRFIENPNVMACYNELLQLACGEVRSQLKLRACNSVFTALEKSQEAIEITSEDHIIQYANPAFESTMGYQSGELIGKELAQVPINEKKGDLLDAINSCVTVDKEWQGVYHTQKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKVETTTECVQTDSQTDNQAGKHKDRRKHSMDAKAVSSRTSDVSSQRRHSSLARIHSMMIEAPITKVINIINAAQENSPVPVTEALNRVLDILRTTELYSPQFNAQDDPHATDLVGGLMSDGLRRFSGNEYILATKNLPPLSNNLATPVSLHDVPPRIALAIENEEQWDFDIFELEVATQNRPLIYLGLKTFARFGMCEFLQCSETTLRSWFQMIESNYHSSNPYHNSTHAADVLHATAYFLSRDKIKETLDRIDEVAALIAATVHDVDHPGRTNSFLCNAGNQLAVLYNDTAVLESHHVALAFQLTLENDQCNIFKQMERNDYRTLRQSIIDMVLATEMTKHFEHVNKFINSINKPLTAQESEEPDRSLEDIKAMLKTPESRALIKRMMIKCADVSNPCRPLEHCIEWAARISEEYFSQTDEEKQLDLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPNLMQHLDDNFRYWKGLDEKKLRSLRPPPE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MGCAPSIHTSENR --CCCCCCCCCCCCC	14.42	25338131
14	Phosphorylation	IHTSENRTFSHSDGE CCCCCCCEECCCCCC	40.86	28833060
16	Phosphorylation	TSENRTFSHSDGEDE CCCCCEECCCCCCCC	22.82	25521595
18	Phosphorylation	ENRTFSHSDGEDEDV CCCEECCCCCCCCCC	46.22	25521595
36	Phosphorylation	VPGPAPRSIQRWSTA CCCCCCCCCCCCCCC	23.24	24719451
41	Phosphorylation	PRSIQRWSTAPGLVE CCCCCCCCCCCCCCC	19.51	27180971
42	Phosphorylation	RSIQRWSTAPGLVEP CCCCCCCCCCCCCCC	29.88	26160508
215	Phosphorylation	RACNSVFTALEKSQE HHHHHHHHHHHHCHH	28.33	22807455
264	Ubiquitination	AQVPINEKKGDLLDA HCCCCCCCCCCHHHH	58.53	27667366
311	Ubiquitination	PVIGQGGKIRHYVSI EEECCCCCEEEEEEE	43.31	-
355	Phosphorylation	HKDRRKHSMDAKAVS CCHHHHHHHCHHHHH	22.76	19367708
369	Phosphorylation	SSRTSDVSSQRRHSS HHCCCCHHHHHHHHH	26.24	27841257
375	Phosphorylation	VSSQRRHSSLARIHS HHHHHHHHHHHHHHH	25.82	22324799
376	Phosphorylation	SSQRRHSSLARIHSM HHHHHHHHHHHHHHH	21.66	22324799
382	Phosphorylation	SSLARIHSMMIEAPI HHHHHHHHHHCCCCH	14.00	27180971
403	Phosphorylation	INAAQENSPVPVTEA HHHHHHCCCCCHHHH	26.90	25293948
420	Phosphorylation	RVLDILRTTELYSPQ HHHHHHHHCCCCCCC	22.60	25293948
421	Phosphorylation	VLDILRTTELYSPQF HHHHHHHCCCCCCCC	19.98	25293948
424	Phosphorylation	ILRTTELYSPQFNAQ HHHHCCCCCCCCCCC	16.45	25293948
425	Phosphorylation	LRTTELYSPQFNAQD HHHCCCCCCCCCCCC	25.50	25293948
452	Phosphorylation	SDGLRRFSGNEYILA CCCCHHCCCCCEEEE	38.61	25521595
456	Phosphorylation	RRFSGNEYILATKNL HHCCCCCEEEEECCC	12.77	25159016
460	Phosphorylation	GNEYILATKNLPPLS CCCEEEEECCCCCCC	19.61	25159016
694	Phosphorylation	ESEEPDRSLEDIKAM CCCCCCCCHHHHHHH	43.41	19060867

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
355	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
355	S	Phosphorylation		-
Phosphorylated at Ser-355 by PKA under elevated cAMP conditions, this enhances catalytic activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PDE8A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of PDE8A_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PDE8A_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-452, AND MASSSPECTROMETRY.	19367708 [Abstract]

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