dbPTM

PDE4A_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PDE4A_RAT
UniProt AC	P54748
Protein Name	cAMP-specific 3',5'-cyclic phosphodiesterase 4A
Gene Name	Pde4a
Organism	Rattus norvegicus (Rat).
Sequence Length	844
Subcellular Localization	Cytoplasm, perinuclear region.
Protein Description	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes..
Protein Sequence	MEPPAAPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYPDSAERSETERSPHRPIERADAVDTGDRPGLRTTRMSWPSSFHGTGTGGGSSRRLEAENGPTPSPGRSPLDSQASPGLVLHAGATTSQRRESFLYRSDSDYDMSPKAVSRSSSVASEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNVPIPSNKRSPLGGPPSVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNEVEIPSPTPRQRAFQQPPPSVLRQSQPMSQITGLKKLVHTGSLNTNVPRFGVKTDQEDLLAQELENLSKWGLNIFCVSEYAGGRSLSCIMYTIFQERDLLKKFHIPVDTMMMYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEENCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNRDWYHSAIRQSPSPPLEEEPGGLGHPSLPDKFQFELTLEEEEEEDSLEVPGLPTTEETFLAAEDARAQAVDWSKVKGPSTTVVEVAERLKQETASAYGAPQESMEAVGCSFSPGTPILPDVRTLSSSEEAPGLLGLPSTAAEVEAPRDHLAATRACSACSGTSGDNSAIISAPGRWGSGGDPA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Phosphorylation	PAAPSERSLSLSLPG CCCCCCCCCCCCCCC	20.47	27097102
13	Phosphorylation	APSERSLSLSLPGPR CCCCCCCCCCCCCCC	19.70	23984901
15	Phosphorylation	SERSLSLSLPGPREG CCCCCCCCCCCCCCC	29.19	23984901
140	Phosphorylation	TTSQRRESFLYRSDS CCHHHHHHHHCCCCC	20.81	27097102
145	Phosphorylation	RESFLYRSDSDYDMS HHHHHCCCCCCCCCC	28.10	22673903
147	Phosphorylation	SFLYRSDSDYDMSPK HHHCCCCCCCCCCHH	39.04	25403869
149	Phosphorylation	LYRSDSDYDMSPKAV HCCCCCCCCCCHHHC	19.93	22673903
152	Phosphorylation	SDSDYDMSPKAVSRS CCCCCCCCHHHCCCC	21.98	25403869
160	Phosphorylation	PKAVSRSSSVASEAH HHHCCCCHHHHCHHH	27.93	-
204	Phosphorylation	PIPSNKRSPLGGPPS CCCCCCCCCCCCCCC	26.53	-
274	Phosphorylation	HLSEMSRSGNQVSEY HHHHHHCCCCHHHHH	34.49	25575281
279	Phosphorylation	SRSGNQVSEYISNTF HCCCCHHHHHHHHHH	18.21	16641100
281	Phosphorylation	SGNQVSEYISNTFLD CCCHHHHHHHHHHHC	11.35	16641100
297	Phosphorylation	QNEVEIPSPTPRQRA CCCCCCCCCCHHHHH	47.25	23800682
333	Phosphorylation	KKLVHTGSLNTNVPR HHHHHCCCCCCCCCC	21.43	25403869
530	Phosphorylation	VIDMVLATDMSKHMT HHHHHHHCCHHHHHH	28.24	22673903
533	Phosphorylation	MVLATDMSKHMTLLA HHHHCCHHHHHHHHH	23.40	22673903
672	Phosphorylation	YHSAIRQSPSPPLEE HHHHHHCCCCCCCCC	19.93	27097102
674	Phosphorylation	SAIRQSPSPPLEEEP HHHHCCCCCCCCCCC	44.38	27097102
688	Phosphorylation	PGGLGHPSLPDKFQF CCCCCCCCCCCCEEE	47.03	27097102
788	Phosphorylation	DVRTLSSSEEAPGLL CCCCCCCCCCCCCCC	35.76	22108457

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
140	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
147	S	Phosphorylation	Kinase	MAPKAPK2	P49137	PSP
147	S	Phosphorylation	Kinase	MAPKAPK2	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PDE4A_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PDE4A_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
AIP_RAT	Aip	physical	21323643

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PDE4A_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphorylation of cAMP-specific PDE4A5 (phosphodiesterase-4A5) byMK2 (MAPKAPK2) attenuates its activation through protein kinase Aphosphorylation."; MacKenzie K.F., Wallace D.A., Hill E.V., Anthony D.F., Henderson D.J.,Houslay D.M., Arthur J.S., Baillie G.S., Houslay M.D.; Biochem. J. 435:755-769(2011). Cited for: PHOSPHORYLATION AT SER-147, AND MUTAGENESIS OF SER-147 AND SER-161.	21323643 [Abstract]