PCKGC_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PCKGC_MOUSE
• UniProt AC: Q9Z2V4
• Protein Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• Gene Name: Pck1 {ECO:0000312|MGI:MGI:97501}
• Organism: Mus musculus (Mouse).
• Sequence Length: 622
• Subcellular Localization: Cytoplasm.
• Protein Description: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle..
• Protein Sequence: MPPQLHNGLDFSAKVIQGSLDSLPQAVRKFVEGNAQLCQPEYIHICDGSEEEYGQLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGISVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDAEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPEGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKDGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYIPKENALNLKGLGGVNVEELFGISKEFWEKEVEEIDRYLEDQVNTDLPYEIERELRALKQRISQM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
19	Phosphorylation	SAKVIQGSLDSLPQA CHHHHHCCHHHHHHH	16.78	25521595
22	Phosphorylation	VIQGSLDSLPQAVRK HHHCCHHHHHHHHHH	47.44	23984901
70	Acetylation	EGVIRKLKKYDNCWL HCHHHHHHHCCCEEE	52.93	-
71	Acetylation	GVIRKLKKYDNCWLA CHHHHHHHCCCEEEE	69.17	-
71	Ubiquitination	GVIRKLKKYDNCWLA CHHHHHHHCCCEEEE	69.17	-
75	S-palmitoylation	KLKKYDNCWLALTDP HHHHCCCEEEEECCH	2.62	28526873
90	Phosphorylation	RDVARIESKTVIITQ HHHHHCEECEEEEEC	31.12	-
91	Ubiquitination	DVARIESKTVIITQE HHHHCEECEEEEECC	33.74	27667366
91	Acetylation	DVARIESKTVIITQE HHHHCEECEEEEECC	33.74	-
91	Malonylation	DVARIESKTVIITQE HHHHCEECEEEEECC	33.74	26320211
92	Phosphorylation	VARIESKTVIITQEQ HHHCEECEEEEECCC	26.64	21183079
107	Ubiquitination	RDTVPIPKTGLSQLG CCCCCCCCCCHHHHC	56.81	27667366
118	Phosphorylation	SQLGRWMSEEDFEKA HHHCCCCCHHHHHHH	30.76	22817900
124	Ubiquitination	MSEEDFEKAFNARFP CCHHHHHHHHHCCCC	59.27	27667366
124	Acetylation	MSEEDFEKAFNARFP CCHHHHHHHHHCCCC	59.27	23954790
135	Ubiquitination	ARFPGCMKGRTMYVI CCCCCCCCCCEEEEE	49.68	-
198	S-palmitoylation	KCLHSVGCPLPLKKP HHHHHCCCCCCCCCC	2.68	28526873
203	Ubiquitination	VGCPLPLKKPLVNNW CCCCCCCCCCCCCCC	50.86	-
204	Ubiquitination	GCPLPLKKPLVNNWA CCCCCCCCCCCCCCC	51.79	-
212	S-palmitoylation	PLVNNWACNPELTLI CCCCCCCCCCCEEEE	7.06	28526873
239	Phosphorylation	GSGYGGNSLLGKKCF CCCCCCCHHHHHHHH	28.39	-
243	Ubiquitination	GGNSLLGKKCFALRI CCCHHHHHHHHHHHH	46.46	-
278	Ubiquitination	TNPEGKKKYLAAAFP CCCCHHHHHHHHHCH	49.32	-
286	Phosphorylation	YLAAAFPSACGKTNL HHHHHCHHCCCCCCC	29.72	29472430
288	S-palmitoylation	AAAFPSACGKTNLAM HHHCHHCCCCCCCCC	7.24	28526873
290	Ubiquitination	AFPSACGKTNLAMMN HCHHCCCCCCCCCCC	33.84	-
307	S-palmitoylation	LPGWKVECVGDDIAW CCCCEEEECCCCEEE	4.67	28526873
316	Ubiquitination	GDDIAWMKFDAQGNL CCCEEEEEEECCCCE	29.41	-
342	Ubiquitination	VAPGTSVKTNPNAIK ECCCCCCCCCCCHHH	42.21	27667366
349	Ubiquitination	KTNPNAIKTIQKNTI CCCCCHHHHHHCCEE	36.85	27667366
389	Ubiquitination	TITSWKNKEWRPQDA EEEECCCCCCCCCCC	55.73	-
407	S-palmitoylation	AHPNSRFCTPASQCP CCCCCCCCCCHHHCC	4.30	28526873
413	S-palmitoylation	FCTPASQCPIIDPAW CCCCHHHCCCCCCCC	2.12	28526873
471	Ubiquitination	ATAAAEHKGKIIMHD HHHHHHHCCEEEEEC	54.10	27667366
473	Acetylation	AAAEHKGKIIMHDPF HHHHHCCEEEEECHH	33.59	-
521	Acetylation	VNWFRKDKDGKFLWP EEEEEECCCCCCCCC	71.99	-
524	Acetylation	FRKDKDGKFLWPGFG EEECCCCCCCCCCCC	47.63	-
524	Ubiquitination	FRKDKDGKFLWPGFG EEECCCCCCCCCCCC	47.63	27667366
551	Ubiquitination	IEGEDSAKLTPIGYI CCCCCCCCEEECEEE	57.77	27667366
560	Ubiquitination	TPIGYIPKENALNLK EECEEECHHHCCCCC	54.78	27667366
567	Ubiquitination	KENALNLKGLGGVNV HHHCCCCCCCCCCCH	51.52	-
587	Ubiquitination	ISKEFWEKEVEEIDR CCHHHHHHHHHHHHH	58.62	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PCKGC_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
90	S	Phosphorylation	Phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes the protein kinase activity: phosphorylated PCK1 translocates to the endoplasmic reticulum, where it phosphorylates INSIG1 and INSIG2.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PCKGC_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of PCKGC_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.