dbPTM

PBS1_ARATH - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PBS1_ARATH
UniProt AC	Q9FE20
Protein Name	Serine/threonine-protein kinase PBS1 {ECO:0000303\|PubMed:11359614}
Gene Name	PBS1 {ECO:0000303\|PubMed:11359614}
Organism	Arabidopsis thaliana (Mouse-ear cress).
Sequence Length	456
Subcellular Localization	Cell membrane Lipid-anchor .
Protein Description	Protein kinase required for plant defense mechanism mediated by the disease resistance (R) protein RPS5. In case of infection by Pseudomonas syringae, AvrPphB triggers RPS5-mediated defense mechanism via the cleavage of PBS1. Both kinase activity and cleavage by avrPphB are independently required to trigger the RPS5-mediated resistance. Contributes to PAMP-triggered immunity (PTI) signaling and defense responses downstream of FLS2..
Protein Sequence	MGCFSCFDSSDDEKLNPVDESNHGQKKQSQPTVSNNISGLPSGGEKLSSKTNGGSKRELLLPRDGLGQIAAHTFAFRELAAATMNFHPDTFLGEGGFGRVYKGRLDSTGQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEFMPLGSLEDHLHDLPPDKEALDWNMRMKIAAGAAKGLEFLHDKANPPVIYRDFKSSNILLDEGFHPKLSDFGLAKLGPTGDKSHVSTRVMGTYGYCAPEYAMTGQLTVKSDVYSFGVVFLELITGRKAIDSEMPHGEQNLVAWARPLFNDRRKFIKLADPRLKGRFPTRALYQALAVASMCIQEQAATRPLIADVVTALSYLANQAYDPSKDDSRRNRDERGARLITRNDDGGGSGSKFDLEGSEKEDSPRETARILNRDINRERAVAEAKMWGESLREKRRQSEQGTSESNSTG

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Myristoylation	------MGCFSCFDS ------CCCCCCCCC	22.60	22046960
3	S-palmitoylation	-----MGCFSCFDSS -----CCCCCCCCCC	1.88	24225654
6	S-palmitoylation	--MGCFSCFDSSDDE --CCCCCCCCCCCCC	1.89	24225654
9	Phosphorylation	GCFSCFDSSDDEKLN CCCCCCCCCCCCCCC	18.53	19880383
10	Phosphorylation	CFSCFDSSDDEKLNP CCCCCCCCCCCCCCC	51.42	19880383
21	Phosphorylation	KLNPVDESNHGQKKQ CCCCCCCCCCCCCCC	29.66	30291188
29	Phosphorylation	NHGQKKQSQPTVSNN CCCCCCCCCCCCCCC	47.18	27545962
32	Phosphorylation	QKKQSQPTVSNNISG CCCCCCCCCCCCCCC	29.00	27545962
34	Phosphorylation	KQSQPTVSNNISGLP CCCCCCCCCCCCCCC	27.00	27545962
38	Phosphorylation	PTVSNNISGLPSGGE CCCCCCCCCCCCCCC	36.08	27545962
42	Phosphorylation	NNISGLPSGGEKLSS CCCCCCCCCCCCCCC	65.55	27545962
160	Phosphorylation	GDQRLLVYEFMPLGS CCCEEEEEEEEECCC	11.92	-
217	Phosphorylation	IYRDFKSSNILLDEG EEECCCCCCEEECCC	28.60	-
244	Phosphorylation	LGPTGDKSHVSTRVM CCCCCCCCCCCCEEC	33.58	25561503
247	Phosphorylation	TGDKSHVSTRVMGTY CCCCCCCCCEECCCC	12.69	30300945
248	Phosphorylation	GDKSHVSTRVMGTYG CCCCCCCCEECCCCC	26.62	-
253	Phosphorylation	VSTRVMGTYGYCAPE CCCEECCCCCEECCC	8.83	27679653
261	Phosphorylation	YGYCAPEYAMTGQLT CCEECCCCEECCCEE	10.70	-
396	Phosphorylation	RNDDGGGSGSKFDLE ECCCCCCCCCCCCCC	43.07	27545962
398	Phosphorylation	DDGGGSGSKFDLEGS CCCCCCCCCCCCCCC	31.67	19880383
405	Phosphorylation	SKFDLEGSEKEDSPR CCCCCCCCCCCCCHH	35.80	30291188
410	Phosphorylation	EGSEKEDSPRETARI CCCCCCCCHHHHHHH	27.27	23111157
437	Phosphorylation	EAKMWGESLREKRRQ HHHHHHHHHHHHHHH	28.87	25561503

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of PBS1_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
3	C	Palmitoylation	22046960
Palmitoylation at Cys-3 and Cys-6 are required for plasma membrane location that is essential for the RPS5-mediated plant defense response.
6	C	Palmitoylation	22046960
Palmitoylation at Cys-3 and Cys-6 are required for plasma membrane location that is essential for the RPS5-mediated plant defense response.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PBS1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
RPS5_ARATH	RPS5	physical	17277084

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PBS1_ARATH

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale Arabidopsis phosphoproteome profiling reveals novelchloroplast kinase substrates and phosphorylation networks."; Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,Grossmann J., Gruissem W., Baginsky S.; Plant Physiol. 150:889-903(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, AND MASSSPECTROMETRY.	19376835 [Abstract]