dbPTM

PARD3_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PARD3_RAT
UniProt AC	Q9Z340
Protein Name	Partitioning defective 3 homolog
Gene Name	Pard3
Organism	Rattus norvegicus (Rat).
Sequence Length	1337
Subcellular Localization	Cytoplasm. Endomembrane system. Cell junction. Cell junction, tight junction . Cell junction, adherens junction . Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell membrane. Localized along the cell-cell contact region. Colocalizes with PARD6A an
Protein Description	Adapter protein involved in asymmetrical cell division and cell polarization processes. [PubMed: 18550519 Seems to play a central role in the formation of epithelial tight junctions (By similarity Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (By similarity The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity Involved in Schwann cell peripheral myelination]
Protein Sequence	MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFRPYQTTSEIEVTPSVLRANMPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTTGSPKTCDRKKDENYRSLPRDPSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRARVIWFHVVPAANKEQYEQLSQREMNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPTAPALAPPNVLSTSVGSVYNTKRVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEPSQMQSPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGESGKCQLSPTVNMPHDDTVMIEDDRLPVLPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSNASQLDFVKTRKSKSMDLGIADETKLNTVDDQRAGSPNRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNGDPEKRDKAEKKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSEEDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYDGCLALNARPQSPREGHLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQSGRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSVSQDSWEQNYAPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Phosphorylation	DGRMKVFSLIQQAVT CCHHHHHHHHHHHHH	27.79	22108457
91	Phosphorylation	PHHGGDGTSASSTGT CCCCCCCCCCCCCCC	26.34	27097102
92	Phosphorylation	HHGGDGTSASSTGTQ CCCCCCCCCCCCCCC	31.51	27097102
94	Phosphorylation	GGDGTSASSTGTQSP CCCCCCCCCCCCCCC	28.35	27097102
95	Phosphorylation	GDGTSASSTGTQSPE CCCCCCCCCCCCCCC	30.49	27097102
96	Phosphorylation	DGTSASSTGTQSPEI CCCCCCCCCCCCCCC	41.58	27097102
98	Phosphorylation	TSASSTGTQSPEIFG CCCCCCCCCCCCCCC	26.43	27097102
100	Phosphorylation	ASSTGTQSPEIFGSE CCCCCCCCCCCCCCC	25.08	27097102
106	Phosphorylation	QSPEIFGSELGTNNV CCCCCCCCCCCCCCC	20.92	27097102
110	Phosphorylation	IFGSELGTNNVSAFR CCCCCCCCCCCCCCC	35.92	27097102
143	Phosphorylation	MPLHVRRSSDPALTG CCEEEECCCCCCHHC	28.64	27097102
144	Phosphorylation	PLHVRRSSDPALTGL CEEEECCCCCCHHCC	45.89	23712012
149	Phosphorylation	RSSDPALTGLSTSVS CCCCCCHHCCCCCCC	37.88	21738781
152	Phosphorylation	DPALTGLSTSVSDNN CCCHHCCCCCCCCCC	21.71	27097102
153	Phosphorylation	PALTGLSTSVSDNNF CCHHCCCCCCCCCCC	37.88	27097102
154	Phosphorylation	ALTGLSTSVSDNNFS CHHCCCCCCCCCCCC	19.09	27097102
156	Phosphorylation	TGLSTSVSDNNFSSE HCCCCCCCCCCCCCC	34.09	29779826
161	Phosphorylation	SVSDNNFSSEEPSRK CCCCCCCCCCCCCCC	38.68	27097102
162	Phosphorylation	VSDNNFSSEEPSRKN CCCCCCCCCCCCCCC	41.67	27097102
166	Phosphorylation	NFSSEEPSRKNPTRW CCCCCCCCCCCCCCC	60.20	28689409
171	Phosphorylation	EPSRKNPTRWSTTAG CCCCCCCCCCCCHHH	54.91	23984901
174	Phosphorylation	RKNPTRWSTTAGFLK CCCCCCCCCHHHHHC	16.76	29779826
175	Phosphorylation	KNPTRWSTTAGFLKQ CCCCCCCCHHHHHCC	17.44	28432305
176	Phosphorylation	NPTRWSTTAGFLKQN CCCCCCCHHHHHCCC	21.19	28432305
187	Phosphorylation	LKQNTTGSPKTCDRK HCCCCCCCCCCCCCC	22.56	25403869
201	Phosphorylation	KKDENYRSLPRDPSS CCCCCCCCCCCCCCH	32.16	29779826
207	Phosphorylation	RSLPRDPSSWSNQFQ CCCCCCCCHHHHHHH	49.05	27097102
208	Phosphorylation	SLPRDPSSWSNQFQR CCCCCCCHHHHHHHH	39.65	27097102
210	Phosphorylation	PRDPSSWSNQFQRDN CCCCCHHHHHHHHHC	23.77	27097102
220	Phosphorylation	FQRDNARSSLSASHP HHHHCHHHHHHHCCH	32.97	16396499
221	Phosphorylation	QRDNARSSLSASHPM HHHCHHHHHHHCCHH	22.31	16396499
223	Phosphorylation	DNARSSLSASHPMVD HCHHHHHHHCCHHHH	29.67	27097102
225	Phosphorylation	ARSSLSASHPMVDRW HHHHHHHCCHHHHHH	25.42	27097102
377	Phosphorylation	SQREMNNYSPGRFSP HHHHHHCCCCCCCCC	15.96	23984901
378	Phosphorylation	QREMNNYSPGRFSPD HHHHHCCCCCCCCCC	24.01	27097102
383	Phosphorylation	NYSPGRFSPDSHCVA CCCCCCCCCCCCCCC	26.83	29779826
386	Phosphorylation	PGRFSPDSHCVANRS CCCCCCCCCCCCCHH	23.36	23984901
485	Phosphorylation	RDVTIGGSAPIYVKN CCEEECCCCCEEEEE	26.17	25575281
489	Phosphorylation	IGGSAPIYVKNILPR ECCCCCEEEEECCCC	12.33	22108457
561	Phosphorylation	REMNAEPSQMQSPKE CCCCCCHHHCCCCCC	29.77	28432305
565	Phosphorylation	AEPSQMQSPKETKAE CCHHHCCCCCCCCCC	32.37	28432305
579	Phosphorylation	EDEDIVLTPDGTREF CCCCEEECCCCCCEE	14.40	22108457
583	Phosphorylation	IVLTPDGTREFLTFE EEECCCCCCEEEEEE	34.11	22673903
692	Phosphorylation	SRCNELRSPGSPAAP HHCHHCCCCCCCCCC	46.36	27097102
695	Phosphorylation	NELRSPGSPAAPELP HHCCCCCCCCCCCCC	18.10	27097102
715	Phosphorylation	DDRERRISHSLYSGI CHHHHHHHHHHHHCC	12.76	29779826
717	Phosphorylation	RERRISHSLYSGIEG HHHHHHHHHHHCCCC	23.39	27097102
719	Phosphorylation	RRISHSLYSGIEGLD HHHHHHHHHCCCCCC	14.10	27097102
720	Phosphorylation	RISHSLYSGIEGLDE HHHHHHHHCCCCCCC	38.29	27097102
728	Phosphorylation	GIEGLDESPTRNAAL CCCCCCCCCCHHHHH	31.10	27097102
730	Phosphorylation	EGLDESPTRNAALSR CCCCCCCCHHHHHHH	45.92	27097102
736	Phosphorylation	PTRNAALSRIMGESG CCHHHHHHHHHCCCC	18.29	23984901
807	Phosphorylation	ISDSADCSLSPDVDP CCCCCCCCCCCCCCH	32.22	22668510
809	Phosphorylation	DSADCSLSPDVDPVL CCCCCCCCCCCCHHH	11.62	22108457
827	Phosphorylation	REGFGRQSMSEKRTK CCCCCCCCCCHHHHH	23.94	29779826
829	Phosphorylation	GFGRQSMSEKRTKQF CCCCCCCCHHHHHHC	45.25	23984901
833	Phosphorylation	QSMSEKRTKQFSNAS CCCCHHHHHHCCCHH	40.02	18267089
834	Acetylation	SMSEKRTKQFSNASQ CCCHHHHHHCCCHHH	54.07	-
837	Phosphorylation	EKRTKQFSNASQLDF HHHHHHCCCHHHHHH	29.06	27097102
840	Phosphorylation	TKQFSNASQLDFVKT HHHCCCHHHHHHHHC	35.21	27097102
850	Phosphorylation	DFVKTRKSKSMDLGI HHHHCCCCCCCCCCC	27.03	28432305
851	Acetylation	FVKTRKSKSMDLGIA HHHCCCCCCCCCCCC	53.48	-
852	Phosphorylation	VKTRKSKSMDLGIAD HHCCCCCCCCCCCCC	25.31	29779826
861	Phosphorylation	DLGIADETKLNTVDD CCCCCCCCCCCCCCC	41.18	22673903
873	Phosphorylation	VDDQRAGSPNRDVGP CCCCCCCCCCCCCCH	20.30	29779826
881	Phosphorylation	PNRDVGPSLGLKKSS CCCCCCHHCCCCCCC	29.19	28689409
885	Acetylation	VGPSLGLKKSSSLES CCHHCCCCCCCCHHH	49.13	-
887	Phosphorylation	PSLGLKKSSSLESLQ HHCCCCCCCCHHHHH	24.46	28689409
888	Phosphorylation	SLGLKKSSSLESLQT HCCCCCCCCHHHHHH	48.07	28689409
889	Phosphorylation	LGLKKSSSLESLQTA CCCCCCCCHHHHHHH	43.16	28689409
892	Phosphorylation	KKSSSLESLQTAVAE CCCCCHHHHHHHEEE	30.94	28689409
924	Phosphorylation	RGRGCNESFRAAIDK CCCCCCHHHHHHHCC	13.17	23984901
947	Phosphorylation	DDDEGMETLEEDTEE CCCCCCCCHHHHCHH	30.49	21630457
958	Phosphorylation	DTEESSRSGRESVST HCHHHHHCCCCCCCC	44.12	25575281
962	Phosphorylation	SSRSGRESVSTSSDQ HHHCCCCCCCCCCCC	21.73	22108457
964	Phosphorylation	RSGRESVSTSSDQPS HCCCCCCCCCCCCCC	31.67	25575281
965	Phosphorylation	SGRESVSTSSDQPSY CCCCCCCCCCCCCCC	30.05	25575281
966	Phosphorylation	GRESVSTSSDQPSYS CCCCCCCCCCCCCCC	25.14	25575281
967	Phosphorylation	RESVSTSSDQPSYSL CCCCCCCCCCCCCCH	40.11	25575281
971	Phosphorylation	STSSDQPSYSLERQM CCCCCCCCCCHHHHH	23.01	25575281
973	Phosphorylation	SSDQPSYSLERQMNG CCCCCCCCHHHHHCC	28.26	28689409
1031	Acetylation	FRFGKHRKDDKMEKM HHHCCCCCCCHHHHC	70.74	26302492
1034	Acetylation	GKHRKDDKMEKMGRI CCCCCCCHHHHCCCE	60.12	26302492
1037	Acetylation	RKDDKMEKMGRIKIQ CCCCHHHHCCCEEEC	43.06	26302492
1042	Acetylation	MEKMGRIKIQDSFTS HHHCCCEEECCCCCC	32.71	26302492
1046	Phosphorylation	GRIKIQDSFTSEEDR CCEEECCCCCCHHHH	17.66	28432305
1048	Phosphorylation	IKIQDSFTSEEDRVR EEECCCCCCHHHHHH	38.90	28432305
1049	Phosphorylation	KIQDSFTSEEDRVRM EECCCCCCHHHHHHH	36.66	28432305
1080	Phosphorylation	RQARERDYAEIQDFH HHHHHHCHHHHHHHH	16.62	27097102
1116	Phosphorylation	ALNARPQSPREGHLM EECCCCCCCCCCCCH	28.71	30417516
1174	Phosphorylation	DVEDRRRTYSFEQSW CHHHHHHHCCHHHHH	23.00	28432305
1175	Phosphorylation	VEDRRRTYSFEQSWS HHHHHHHCCHHHHHC	15.00	28432305
1176	Phosphorylation	EDRRRTYSFEQSWSS HHHHHHCCHHHHHCC	22.99	23984901
1219	Phosphorylation	FQQAQRQYSSLPRQS HHHHHHHHHCCCHHH	11.47	30181290
1220	Phosphorylation	QQAQRQYSSLPRQSR HHHHHHHHCCCHHHC	19.63	28432305
1221	Phosphorylation	QAQRQYSSLPRQSRK HHHHHHHCCCHHHCC	37.14	28432305
1231	Phosphorylation	RQSRKNASSVSQDSW HHHCCCCCCCCCCHH	40.44	25575281
1232	Phosphorylation	QSRKNASSVSQDSWE HHCCCCCCCCCCHHH	24.31	25575281
1234	Phosphorylation	RKNASSVSQDSWEQN CCCCCCCCCCHHHHH	30.16	28689409
1237	Phosphorylation	ASSVSQDSWEQNYAP CCCCCCCHHHHHCCC	25.52	28689409
1242	Phosphorylation	QDSWEQNYAPGEGFQ CCHHHHHCCCCCCCC	17.54	25575281
1316	Phosphorylation	FRQDVPPSPSQVARL CCCCCCCCHHHHHHH	30.98	27097102
1318	Phosphorylation	QDVPPSPSQVARLNR CCCCCCHHHHHHHHC	41.44	28432305
1331	Acetylation	NRLQTPEKGRPFYS- HCCCCCCCCCCCCC-	62.51	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
827	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
827	S	Phosphorylation	Kinase	PKCZ	Q02956	PSP
833	T	Phosphorylation	Kinase	ROCK1	Q13464	PSP
962	S	Phosphorylation	Kinase	AURKA	P59241	Uniprot
1116	S	Phosphorylation	Kinase	MAPK1	P63085	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
827	S	Phosphorylation	12045219
Phosphorylation at Ser-827 by PRKCZ and PRKCI occurs at the most apical tip of epithelial cell-cell contacts during the initial phase of tight junction formation and may promote dissociation of the complex with PARD6.
962	S	Phosphorylation	-
Phosphorylation by AURKA at Ser-962 is required for the normal establishment of neuronal polarity (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PARD3_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of PARD3_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PARD3_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Involvement of ASIP/PAR-3 in the promotion of epithelial tightjunction formation."; Hirose T., Izumi Y., Nagashima Y., Tamai-Nagai Y., Kurihara H.,Sakai T., Suzuki Y., Yamanaka T., Suzuki A., Mizuno K., Ohno S.; J. Cell Sci. 115:2485-2495(2002). Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, ANDPHOSPHORYLATION AT SER-827.	12045219 [Abstract]

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