PACN1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PACN1_RAT
• UniProt AC: Q9Z0W5
• Protein Name: Protein kinase C and casein kinase substrate in neurons protein 1
• Gene Name: Pacsin1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 441
• Subcellular Localization: Cytoplasm. Cell projection. Cell junction, synapse, synaptosome. Cell projection, ruffle membrane. Membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Cell junction, synapse. Cytoplasm, cytosol. Cell membrane
• Protein Description: Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission..
• Protein Sequence: MSGPYDEASEEITDSFWEVGNYKRTVKRIDDGHRLCNDLMSCVQERAKIEKAYAQQLTDWAKRWRQLIEKGPQYGSLERAWGAMMTEADKVSELHQEVKNSLLNEDLEKVKNWQKDAYHKQIMGGFKETKEAEDGFRKAQKPWAKKMKELEAAKKAYHLACKEEKLAMTREMNSKTEQSVTPEQQKKLVDKVDKCRQDVQKTQEKYEKVLEDVGKTTPQYMEGMEQVFEQCQQFEEKRLVFLKEVLLDIKRHLNLAENSSYIHVYRELEQAIRGADAQEDLRWFRSTSGPGMPMNWPQFEEWNPDLPHTAAKKEKQPKKAEGAALSNATGAVESTSQAGDRGSVSSYDRGQAYATEWSDDESGNPFGGNEANGGANPFEDDAKGVRVRALYDYDGQEQDELSFKAGDELTKLGEEDEQGWCRGRLDSGQLGLYPANYVEAI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSGPYDEAS ------CCCCCHHHH	48.77	22673903
9	Phosphorylation	SGPYDEASEEITDSF CCCCHHHHHHHHHHH	33.44	22673903
51	Acetylation	QERAKIEKAYAQQLT HHHHHHHHHHHHHHH	50.61	22902405
62	Acetylation	QQLTDWAKRWRQLIE HHHHHHHHHHHHHHH	48.52	22902405
76	Phosphorylation	EKGPQYGSLERAWGA HHCCCCCCHHHHHHH	23.32	22355135
90	Acetylation	AMMTEADKVSELHQE HHCCHHHHHHHHHHH	55.50	22902405
99	Ubiquitination	SELHQEVKNSLLNED HHHHHHHHHHHCHHH	39.81	-
109	Acetylation	LLNEDLEKVKNWQKD HCHHHHHHHHHHHHH	66.85	22902405
115	Acetylation	EKVKNWQKDAYHKQI HHHHHHHHHHHHHHH	35.62	22902405
127	Acetylation	KQIMGGFKETKEAED HHHHHCCCCCHHHHH	68.68	22902405
145	Acetylation	KAQKPWAKKMKELEA HHCCHHHHHHHHHHH	49.91	22902405
162	Acetylation	KAYHLACKEEKLAMT HHHHHHHHHHHHHHH	64.40	22902405
165	Acetylation	HLACKEEKLAMTREM HHHHHHHHHHHHHHH	42.51	22902405
179	Phosphorylation	MNSKTEQSVTPEQQK HHHCCCCCCCHHHHH	23.02	27115346
181	Phosphorylation	SKTEQSVTPEQQKKL HCCCCCCCHHHHHHH	26.76	30411139
187	Acetylation	VTPEQQKKLVDKVDK CCHHHHHHHHHHHHH	49.78	22902405
191	Acetylation	QQKKLVDKVDKCRQD HHHHHHHHHHHHHHH	44.87	22902405
208	Acetylation	KTQEKYEKVLEDVGK HHHHHHHHHHHHHHC	49.37	72592373
250	Acetylation	KEVLLDIKRHLNLAE HHHHHHHHHHHCHHC	33.17	22902405
326	Phosphorylation	KAEGAALSNATGAVE HHHHCHHCCCCCCCC	21.49	23298284
329	Phosphorylation	GAALSNATGAVESTS HCHHCCCCCCCCCCC	29.89	28551015
334	Phosphorylation	NATGAVESTSQAGDR CCCCCCCCCCCCCCC	27.10	23298284
335	Phosphorylation	ATGAVESTSQAGDRG CCCCCCCCCCCCCCC	16.02	23298284
336	Phosphorylation	TGAVESTSQAGDRGS CCCCCCCCCCCCCCC	27.58	25403869
343	Phosphorylation	SQAGDRGSVSSYDRG CCCCCCCCCCCCCCC	21.51	30411139
345	Phosphorylation	AGDRGSVSSYDRGQA CCCCCCCCCCCCCCC	25.52	30411139
346	Phosphorylation	GDRGSVSSYDRGQAY CCCCCCCCCCCCCCC	28.97	30411139
347	Phosphorylation	DRGSVSSYDRGQAYA CCCCCCCCCCCCCCC	11.36	23298284
355	Phosphorylation	DRGQAYATEWSDDES CCCCCCCEECCCCCC	25.88	28551015
358	Phosphorylation	QAYATEWSDDESGNP CCCCEECCCCCCCCC	29.02	30411139
362	Phosphorylation	TEWSDDESGNPFGGN EECCCCCCCCCCCCC	51.36	28551015
391	Phosphorylation	GVRVRALYDYDGQEQ CCEEEEEECCCCCCC	16.09	-
402	Phosphorylation	GQEQDELSFKAGDEL CCCCCEEEECCCCHH	23.74	28551015
427	Phosphorylation	WCRGRLDSGQLGLYP CCCCCCCCCCCCCEE	32.79	22355135

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
355	T	Phosphorylation	Kinase	CAMKK1	Q8N5S9	PSP
355	T	Phosphorylation	Kinase	CAMKK2	Q96RR4	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PACN1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PACN1_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of PACN1_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.