P85B_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: P85B_MOUSE
• UniProt AC: O08908
• Protein Name: Phosphatidylinositol 3-kinase regulatory subunit beta
• Gene Name: Pik3r2
• Organism: Mus musculus (Mouse).
• Sequence Length: 722
• Protein Description: Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (By similarity). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. [PubMed: 20348926]
• Protein Sequence: MAGAEGFQYRAVYPFRRERPEDLELLPGDLLVVSRVALQALGVADGGERCPHNVGWMPGFNERTRQRGDFPGTYVEFLGPVALARPGPRPRGPRPLPARPLDGSSESGHILPDLAEQFSPPDPAPPILVKLVEAIEQAELDSECYSKPELPATRTDWSLSDLEQWDRTALYDAVKGFLLALPAAVVTPEAAAEAYRALREVAGPVGLVLEPPTLPLHQALTLRFLLQHLGRVARRAPSPDTAVHALASAFGPLLLRIPPSGGEGDGSEPVPDFPVLLLERLVQEHVEEQDAAPPALPPKPSKAKPAPTALANGGSPPSLQDAEWYWGDISREEVNERLRDTPDGTFLVRDASSKIQGEYTLTLRKGGNNKLIKVFHRDGHYGFSEPLTFCSVVELISHYRHESLAQYNAKLDTRLLYPVSKYQQDQVVKEDSIEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQGQTQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQDLRAQASDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGARQRKINEWLGIKNETEDQYSLMEDEDALPHHEERTWYVGKINRTQAEEMLSGKRDGTFLIRESSQRGCYACSVVVDGDTKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHASLVQHNDALTVTLAHPVRAPGPGPPSAAR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	AGAEGFQYRAVYPFR CCCCCCCEEEEEEEC	9.77	29514104
13	Phosphorylation	GFQYRAVYPFRRERP CCCEEEEEEECCCCC	8.82	29514104
50	Glutathionylation	VADGGERCPHNVGWM CCCCCCCCCCCCCCC	3.32	24333276
73	Phosphorylation	QRGDFPGTYVEFLGP HCCCCCCCCEEECCC	25.32	29514104
74	Phosphorylation	RGDFPGTYVEFLGPV CCCCCCCCEEECCCH	12.14	29514104
195	Phosphorylation	PEAAAEAYRALREVA HHHHHHHHHHHHHHH	6.47	-
238	Phosphorylation	RVARRAPSPDTAVHA HHHHHCCCHHHHHHH	34.13	29899451
267	Phosphorylation	SGGEGDGSEPVPDFP CCCCCCCCCCCCCCH	43.39	25338131
359	Phosphorylation	SSKIQGEYTLTLRKG CCCCCEEEEEEEEEC	17.55	25338131
360	Phosphorylation	SKIQGEYTLTLRKGG CCCCEEEEEEEEECC	14.68	22942356
417	Phosphorylation	KLDTRLLYPVSKYQQ CCCCHHHCCCCHHCC	13.23	-
443	Phosphorylation	VGAQLKVYHQQYQDK HHHHHHHHHHHHHHH	7.72	25195567
447	Phosphorylation	LKVYHQQYQDKSREY HHHHHHHHHHHHHHH	16.47	29514104
454	Phosphorylation	YQDKSREYDQLYEEY HHHHHHHHHHHHHHH	14.10	22499769
458	Phosphorylation	SREYDQLYEEYTRTS HHHHHHHHHHHHHHH	10.90	18515860
458	Nitration	SREYDQLYEEYTRTS HHHHHHHHHHHHHHH	10.90	-
461	Phosphorylation	YDQLYEEYTRTSQEL HHHHHHHHHHHHHHH	6.92	22817900
499	Phosphorylation	QEKCSKEYLERFRRE HHHHCHHHHHHHHHH	20.01	29514104
571	Phosphorylation	LRKIRDQYLVWLTQK HHHHHHHHHHHHCHH	13.91	29514104
599	Phosphorylation	KNETEDQYSLMEDED CCCCHHHHHCCCCCC	18.98	22499769
600	Phosphorylation	NETEDQYSLMEDEDA CCCHHHHHCCCCCCC	18.66	22499769
637	Phosphorylation	LSGKRDGTFLIRESS HCCCCCCCEEEEECC	21.40	-
649	Phosphorylation	ESSQRGCYACSVVVD ECCCCCEEEEEEEEC	17.29	-
652	Phosphorylation	QRGCYACSVVVDGDT CCCEEEEEEEECCCC	15.13	-
665	Phosphorylation	DTKHCVIYRTATGFG CCCEEEEEEECCCCC	4.98	-
682	Phosphorylation	EPYNLYGSLKELVLH CCCHHHHHHHHHHHH	23.78	23140645

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of P85B_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of P85B_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of P85B_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KIT_MOUSE	Kit	physical	22378847

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain.";
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
J. Proteome Res. 7:311-318(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, AND MASSSPECTROMETRY.
PubMed: 18034455

"Quantitative time-resolved phosphoproteomic analysis of mast cellsignaling.";
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y.,Kawakami T., Salomon A.R.;
J. Immunol. 179:5864-5876(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-458, AND MASSSPECTROMETRY.
PubMed: 17947660