NUCB1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NUCB1_MOUSE
• UniProt AC: Q02819
• Protein Name: Nucleobindin-1
• Gene Name: Nucb1
• Organism: Mus musculus (Mouse).
• Sequence Length: 459
• Subcellular Localization: Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein ; Lumenal side . Cytoplasm . Secreted . A small fraction of the protein may be cytoplasmic. In bone at least part of it is found in the extracellular matrix and in the culture
• Protein Description: Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis (By similarity)..
• Protein Sequence: MPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPPQEDSQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWKTVEMSPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQLQEQSAPPSKPDGQLQFRADTDDAPVPAPAGDQKDVPASEKKVPEQPPELPQLDSQHL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
38	Phosphorylation	AAPPQEDSQATETPD CCCCCCCCCCCCCCC	22.44	29899451
81	Phosphorylation	ANAEDIKSGKLSQEL CCHHHHHCCCCCHHH	41.30	25177544
85	Phosphorylation	DIKSGKLSQELDFVS HHHCCCCCHHHHHHH	25.70	26824392
147	Phosphorylation	LDPQNQHTFEARDLE CCCCCCCCCHHHHHH	17.26	23140645
178	Phosphorylation	HHEEFKRYEMLKEHE CHHHHHHHHHHHHHH	13.28	-
189	Phosphorylation	KEHERRRYLESLGEE HHHHHHHHHHHHCHH	17.01	18779572
223	Phosphorylation	PKVNVPGSQAQLKEV CCCCCCCCHHHHHHH	19.10	27841257
334	Phosphorylation	DTGEGWKTVEMSPAY CCCCCCEEEECCCCC	18.39	28973931
368	Phosphorylation	NARAQRLSQETEALG HHHHHHHHHHHHHHH	28.32	27087446
371	Phosphorylation	AQRLSQETEALGRSQ HHHHHHHHHHHHCCH	20.94	26239621
406	O-linked_Glycosylation	KQQLQEQSAPPSKPD HHHHHHCCCCCCCCC	41.83	22645316
410	O-linked_Glycosylation	QEQSAPPSKPDGQLQ HHCCCCCCCCCCCEE	56.62	55412993
456	Phosphorylation	PELPQLDSQHL---- CCCCCCCCCCC----	28.26	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NUCB1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NUCB1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NUCB1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of NUCB1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry.";
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
Mol. Cell. Proteomics 8:904-912(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, AND MASSSPECTROMETRY.
PubMed: 19131326

"The phagosomal proteome in interferon-gamma-activated macrophages.";
Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.;
Immunity 30:143-154(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456, AND MASSSPECTROMETRY.
PubMed: 19144319

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456, AND MASSSPECTROMETRY.
PubMed: 19367708

"Specific phosphopeptide enrichment with immobilized titanium ionaffinity chromatography adsorbent for phosphoproteome analysis.";
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.;
J. Proteome Res. 7:3957-3967(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, AND MASSSPECTROMETRY.
PubMed: 18630941

"Mitochondrial phosphoproteome revealed by an improved IMAC method andMS/MS/MS.";
Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y.;
Mol. Cell. Proteomics 6:669-676(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, AND MASSSPECTROMETRY.
PubMed: 17208939

"Phosphoproteome analysis of mouse liver using immobilized metalaffinity purification and linear ion trap mass spectrometry.";
Jin W.-H., Dai J., Zhou H., Xia Q.-C., Zou H.-F., Zeng R.;
Rapid Commun. Mass Spectrom. 18:2169-2176(2004).
Cited for: PHOSPHORYLATION AT SER-85.
PubMed: 15378723