NSF_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NSF_RAT
• UniProt AC: Q9QUL6
• Protein Name: Vesicle-fusing ATPase
• Gene Name: Nsf
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 744
• Subcellular Localization: Cytoplasm.
• Protein Description: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling..
• Protein Sequence: MAGRTMQAARCPTDELSLSNCAVVNEKDYQSGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNHQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASPLDFD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
27	Ubiquitination	NCAVVNEKDYQSGQH CCEEECCCCCCCCCE	57.44	-
44	Acetylation	VRTSPNHKYIFTLRT EECCCCCCEEEEEEC	46.83	22902405
105	Acetylation	EIDFLQKKNIDSNPY EEEEHHHCCCCCCCC	46.29	-
109	Phosphorylation	LQKKNIDSNPYDTDK HHHCCCCCCCCCHHH	35.59	22673903
161	Ubiquitination	AMDPSILKGEPASGK HCCHHHHCCCCCCCC	60.60	-
168	Ubiquitination	KGEPASGKRQKIEVG CCCCCCCCCCEEEEE	49.51	-
198	Ubiquitination	SSLNLIGKAKTKENR CCCCCCEECCCHHHH	39.45	-
207	Phosphorylation	KTKENRQSIINPDWN CCHHHHHCCCCCCCC	23.38	28432305
217	Ubiquitination	NPDWNFEKMGIGGLD CCCCCHHHCCCCCCC	37.99	-
225	Ubiquitination	MGIGGLDKEFSDIFR CCCCCCCHHHHHHHH	66.75	-
254	Ubiquitination	QMGCKHVKGILLYGP HHCCCEEEEEEEECC	39.70	-
259	Phosphorylation	HVKGILLYGPPGCGK EEEEEEEECCCCCCH	24.92	-
266	Ubiquitination	YGPPGCGKTLLARQI ECCCCCCHHHHHHHH	39.82	-
275	Ubiquitination	LLARQIGKMLNAREP HHHHHHHHHHCCCCC	41.67	-
283	Ubiquitination	MLNAREPKVVNGPEI HHCCCCCCCCCCHHH	54.80	-
293	Acetylation	NGPEILNKYVGESEA CCHHHHHHHCCCCHH	37.56	22902405
293	Ubiquitination	NGPEILNKYVGESEA CCHHHHHHHCCCCHH	37.56	-
294	Phosphorylation	GPEILNKYVGESEAN CHHHHHHHCCCCHHH	16.77	-
304	Acetylation	ESEANIRKLFADAEE CCHHHHHHHHCCHHH	44.13	22902405
335	Ubiquitination	DEIDAICKQRGSMAG CCHHHHHHHCCCCCC	36.60	-
339	Phosphorylation	AICKQRGSMAGSTGV HHHHHCCCCCCCCCC	13.98	25403869
343	Phosphorylation	QRGSMAGSTGVHDTV HCCCCCCCCCCHHHH	16.67	22673903
344	Phosphorylation	RGSMAGSTGVHDTVV CCCCCCCCCCHHHHH	42.16	22673903
349	Phosphorylation	GSTGVHDTVVNQLLS CCCCCHHHHHHHHHH	16.10	22673903
392	Ubiquitination	RPGRLEVKMEIGLPD CCCCEEEEEEECCCC	22.80	-
401	Acetylation	EIGLPDEKGRLQILH EECCCCCCCCEEEEE	57.63	22902405
401	Ubiquitination	EIGLPDEKGRLQILH EECCCCCCCCEEEEE	57.63	-
421	Phosphorylation	MRGHQLLSADVDIKE HCCCEEECCCCCHHH	30.89	-
427	Ubiquitination	LSADVDIKELAVETK ECCCCCHHHHHHCCC	42.06	-
434	Acetylation	KELAVETKNFSGAEL HHHHHCCCCCCHHHH	42.35	2641199
434	Ubiquitination	KELAVETKNFSGAEL HHHHHCCCCCCHHHH	42.35	-
437	Phosphorylation	AVETKNFSGAELEGL HHCCCCCCHHHHHHH	46.62	23984901
450	Phosphorylation	GLVRAAQSTAMNRHI HHHHHHHHHHHHHHH	17.26	25403869
451	Phosphorylation	LVRAAQSTAMNRHIK HHHHHHHHHHHHHHC	20.18	25403869
462	Ubiquitination	RHIKASTKVEVDMEK HHHCCCCEEEECHHH	33.62	-
469	Ubiquitination	KVEVDMEKAESLQVT EEEECHHHHHHCCCC	51.31	-
472	Phosphorylation	VDMEKAESLQVTRGD ECHHHHHHCCCCCHH	29.03	25403869
499	Phosphorylation	FGTNQEDYASYIMNG CCCCHHHHHHHHHHC	9.19	-
529	Ubiquitination	ELLVQQTKNSDRTPL CEEEEECCCCCCCCE	50.55	-
549	Ubiquitination	EGPPHSGKTALAAKI CCCCCCCHHHHHHHH	33.37	-
555	Ubiquitination	GKTALAAKIAEESNF CHHHHHHHHHHHCCC	36.52	-
566	Ubiquitination	ESNFPFIKICSPDKM HCCCCEEEECCCCCC	37.73	-
569	Phosphorylation	FPFIKICSPDKMIGF CCEEEECCCCCCCCC	39.34	-
572	Ubiquitination	IKICSPDKMIGFSET EEECCCCCCCCCCHH	35.48	-
581	Ubiquitination	IGFSETAKCQAMKKI CCCCHHHHHHHHHHH	34.01	-
581	Acetylation	IGFSETAKCQAMKKI CCCCHHHHHHHHHHH	34.01	2632995
587	Acetylation	AKCQAMKKIFDDAYK HHHHHHHHHHCHHHH	35.20	22902405
594	Ubiquitination	KIFDDAYKSQLSCVV HHHCHHHHHCCCEEE	33.18	-
611	Phosphorylation	DIERLLDYVPIGPRF CHHHHHHCCCCCHHH	14.22	-
699	Acetylation	TTIAQQVKGKKVWIG CCHHHHHCCCEEEEH	61.75	2641203
699	Ubiquitination	TTIAQQVKGKKVWIG CCHHHHHCCCEEEEH	61.75	-
739	Phosphorylation	LMREEGASPLDFD-- HHHHCCCCCCCCC--	36.53	28551015

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
569	S	Phosphorylation	Kinase	CDK16	Q63686	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
569	S	Phosphorylation	Phosphorylation at Ser-569 interferes with homohexamerization.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NSF_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of NSF_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.