NPL4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NPL4_MOUSE
• UniProt AC: P60670
• Protein Name: Nuclear protein localization protein 4 homolog
• Gene Name: Nploc4
• Organism: Mus musculus (Mouse).
• Sequence Length: 608
• Subcellular Localization: Cytoplasm, cytosol . Endoplasmic reticulum . Nucleus . Associated with the endoplasmic reticulum and nuclear.
• Protein Description: The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity)..
• Protein Sequence: MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSSKSLHLLKIKHGDLLFLFPSSLAGPSSEMETSTSVGLKAFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNICRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDIDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKKSEQWATIEQLCSTVGVQLPGLHEFGAVGGSARAATSAMWACQHCTFMNQPGTGHCEMCSLPRT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAESIIIRV ------CCCEEEEEE	21.17	-
62	Ubiquitination	EITASSSKSLHLLKI CEEECCCCCEEEEEE	60.01	-
62	Malonylation	EITASSSKSLHLLKI CEEECCCCCEEEEEE	60.01	26320211
63	Phosphorylation	ITASSSKSLHLLKIK EEECCCCCEEEEEEE	23.99	29899451
68	Ubiquitination	SKSLHLLKIKHGDLL CCCEEEEEEECCCEE	56.95	22790023
116	Ubiquitination	EIDQYLSKQDGKIYR HHHHHHHHCCCCEEE	50.22	22790023
120	Ubiquitination	YLSKQDGKIYRSRDP HHHHCCCCEEECCCH	44.87	-
179	Acetylation	TGGADKGKFVALENI HCCCCCCCEEEEEEE	42.26	23806337
188	S-nitrosylation	VALENISCKIKSGCE EEEEEEEEEEECCCC	4.71	21278135
188	S-nitrosocysteine	VALENISCKIKSGCE EEEEEEEEEEECCCC	4.71	-
243	Ubiquitination	RFLDFWRKTGNQHFG HHHHHHHHHCCCCCE	51.91	22790023
260	Ubiquitination	YGRYTEHKDIPLGIR ECCCCCCCCCCCCEE	51.63	22790023
302	Ubiquitination	VVDEIAAKLGLRKVG HHHHHHHHHCCCHHH	33.78	22790023
307	Ubiquitination	AAKLGLRKVGWIFTD HHHHCCCHHHEEEEE	50.58	22790023
431	Acetylation	VFYKDIDKFGNEITQ HHHHCHHHHHHHHHH	56.35	23954790
431	Ubiquitination	VFYKDIDKFGNEITQ HHHHCHHHHHHHHHH	56.35	-
544	Ubiquitination	EELAQTWKKSEQWAT HHHHHHHHHHHHHHH	50.21	22790023
545	Ubiquitination	ELAQTWKKSEQWATI HHHHHHHHHHHHHHH	51.27	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NPL4_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NPL4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NPL4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBC_HUMAN	UBC	physical	12411482
TERA_MOUSE	Vcp	physical	17491009

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.