dbPTM

NOLC1_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NOLC1_RAT
UniProt AC	P41777
Protein Name	Nucleolar and coiled-body phosphoprotein 1 {ECO:0000305}
Gene Name	Nolc1 {ECO:0000312\|RGD:621578}
Organism	Rattus norvegicus (Rat).
Sequence Length	704
Subcellular Localization	Cytoplasm . Nucleus, nucleolus . Shuttles on curvilinear tracks between nucleolus and cytoplasm. These tracks extend from the dense fibrillar component of the nucleolus across the nucleoplasm to a limited number of nuclear pore complexes.
Protein Description	Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities..
Protein Sequence	MADTGLRRVVPSDLYPLVLGFLRDNQLSEVASKFAKATGATQQDANASSLLDIYSFWLKSTKAPKVKLQSNGPVAKKAKKETSSSDSSEDSSEEEDKAQVPTQKAAAPAKRASLPQHAGKAAAKASESSSSEESSEEEEEEDKKKKPVQQKAVKPQAKAVRPPPKKAESSESESDSSSEDEAPQTQKPKAAATAAKAPTKAQTKAPAKPGPPAKAQPKAANGKAGSSSSSSSSSSSDDSEEEKKAAAPLKKTAPKKQVVAKAPVKVTAAPTQKSSSSEDSSSEEEEEQKKPMKKKAGPYSSVPPPSVSLSKKSVGAQSPKKAAAQTQPADSSADSSEESDSSSEEEKKTPAKTVVSKTPAKPAPVKKKAESSSDSSDSDSSEDEAPAKPVSATKSPLSKPAVTPKPPAAKAVATPKQPAGSGQKPQSRKADSSSSEEESSSSEEEATKKSVTTPKARVTAKAAPSLPAKQAPRAGGDSSSDSESSSSEEEKKTPPKPPAKKKAAGAAVPKPTPVKKAAAESSSSSSSSEDSSEEEKKKPKSKATPKPQAGKANGVPASQNGKAGKESEEEEEDTEQNKKAAGTKPGSGKKRKHNETADEAATPQSKKVKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFDSE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
33	Acetylation	QLSEVASKFAKATGA CHHHHHHHHHHHHCC	39.33	-
33	Acetylation	QLSEVASKFAKATGA CHHHHHHHHHHHHCC	39.33	22902405
76	Acetylation	QSNGPVAKKAKKETS CCCCHHHHHHHHHCC	54.20	-
76	Acetylation	QSNGPVAKKAKKETS CCCCHHHHHHHHHCC	54.20	25786129
87	Phosphorylation	KETSSSDSSEDSSEE HHCCCCCCCCCCCHH	37.26	-
88	Phosphorylation	ETSSSDSSEDSSEEE HCCCCCCCCCCCHHH	50.87	28689409
91	Phosphorylation	SSDSSEDSSEEEDKA CCCCCCCCCHHHHHH	35.28	28689409
92	Phosphorylation	SDSSEDSSEEEDKAQ CCCCCCCCHHHHHHC	62.44	28689409
113	Phosphorylation	AAPAKRASLPQHAGK HHCHHHCCCCHHHHH	44.61	12167624
120	Acetylation	SLPQHAGKAAAKASE CCCHHHHHHHHHHHH	35.32	-
120	Acetylation	SLPQHAGKAAAKASE CCCHHHHHHHHHHHH	35.32	22902405
165	Acetylation	KAVRPPPKKAESSES HHCCCCCCCCCCCCC	71.09	-
165	Acetylation	KAVRPPPKKAESSES HHCCCCCCCCCCCCC	71.09	22902405
174	Phosphorylation	AESSESESDSSSEDE CCCCCCCCCCCCCCC	52.66	28689409
176	Phosphorylation	SSESESDSSSEDEAP CCCCCCCCCCCCCCC	45.07	28689409
177	Phosphorylation	SESESDSSSEDEAPQ CCCCCCCCCCCCCCC	42.61	28689409
178	Phosphorylation	ESESDSSSEDEAPQT CCCCCCCCCCCCCCC	53.68	28689409
196	Acetylation	KAAATAAKAPTKAQT HHHHHHHCCCCHHHC	53.05	25786129
208	Acetylation	AQTKAPAKPGPPAKA HHCCCCCCCCCCCCC	49.82	-
208	Acetylation	AQTKAPAKPGPPAKA HHCCCCCCCCCCCCC	49.82	22902405
261	Acetylation	PKKQVVAKAPVKVTA CCCCEEEECCEEEEE	41.43	-
261	Acetylation	PKKQVVAKAPVKVTA CCCCEEEECCEEEEE	41.43	25786129
267	Phosphorylation	AKAPVKVTAAPTQKS EECCEEEEECCCCCC	16.04	23984901
271	Phosphorylation	VKVTAAPTQKSSSSE EEEEECCCCCCCCCC	43.85	23984901
274	Phosphorylation	TAAPTQKSSSSEDSS EECCCCCCCCCCCCC	26.15	23984901
275	Phosphorylation	AAPTQKSSSSEDSSS ECCCCCCCCCCCCCC	44.94	23984901
276	Phosphorylation	APTQKSSSSEDSSSE CCCCCCCCCCCCCCH	44.85	23984901
277	Phosphorylation	PTQKSSSSEDSSSEE CCCCCCCCCCCCCHH	47.58	23984901
280	Phosphorylation	KSSSSEDSSSEEEEE CCCCCCCCCCHHHHH	31.54	28689409
281	Phosphorylation	SSSSEDSSSEEEEEQ CCCCCCCCCHHHHHH	54.83	28689409
282	Phosphorylation	SSSEDSSSEEEEEQK CCCCCCCCHHHHHHH	53.64	28689409
311	Acetylation	PPSVSLSKKSVGAQS CCCCCCCCCCCCCCC	55.12	-
311	Acetylation	PPSVSLSKKSVGAQS CCCCCCCCCCCCCCC	55.12	22902405
313	Phosphorylation	SVSLSKKSVGAQSPK CCCCCCCCCCCCCHH	29.53	25403869
318	Phosphorylation	KKSVGAQSPKKAAAQ CCCCCCCCHHHHHHH	37.85	29779826
326	Phosphorylation	PKKAAAQTQPADSSA HHHHHHHCCCCCCCC	31.62	28689409
331	Phosphorylation	AQTQPADSSADSSEE HHCCCCCCCCCCCCC	29.54	28689409
332	Phosphorylation	QTQPADSSADSSEES HCCCCCCCCCCCCCC	36.32	28689409
335	Phosphorylation	PADSSADSSEESDSS CCCCCCCCCCCCCCC	39.09	23984901
336	Phosphorylation	ADSSADSSEESDSSS CCCCCCCCCCCCCCC	46.03	23984901
339	Phosphorylation	SADSSEESDSSSEEE CCCCCCCCCCCCHHH	38.45	23984901
341	Phosphorylation	DSSEESDSSSEEEKK CCCCCCCCCCHHHHC	45.07	23984901
342	Phosphorylation	SSEESDSSSEEEKKT CCCCCCCCCHHHHCC	46.92	23984901
343	Phosphorylation	SEESDSSSEEEKKTP CCCCCCCCHHHHCCC	53.64	23984901
352	Acetylation	EEKKTPAKTVVSKTP HHHCCCCCEEECCCC	42.95	-
352	Acetylation	EEKKTPAKTVVSKTP HHHCCCCCEEECCCC	42.95	22902405
366	Acetylation	PAKPAPVKKKAESSS CCCCCCCCCCCCCCC	47.71	-
366	Acetylation	PAKPAPVKKKAESSS CCCCCCCCCCCCCCC	47.71	22902405
371	Phosphorylation	PVKKKAESSSDSSDS CCCCCCCCCCCCCCC	39.75	28689409
372	Phosphorylation	VKKKAESSSDSSDSD CCCCCCCCCCCCCCC	29.66	28689409
373	Phosphorylation	KKKAESSSDSSDSDS CCCCCCCCCCCCCCC	51.28	28689409
375	Phosphorylation	KAESSSDSSDSDSSE CCCCCCCCCCCCCCC	37.68	28689409
376	Phosphorylation	AESSSDSSDSDSSED CCCCCCCCCCCCCCC	46.23	23800682
378	Phosphorylation	SSSDSSDSDSSEDEA CCCCCCCCCCCCCCC	41.14	28689409
380	Phosphorylation	SDSSDSDSSEDEAPA CCCCCCCCCCCCCCC	39.31	28689409
381	Phosphorylation	DSSDSDSSEDEAPAK CCCCCCCCCCCCCCC	54.80	28689409
395	Phosphorylation	KPVSATKSPLSKPAV CCCCCCCCCCCCCCC	27.07	27097102
398	Phosphorylation	SATKSPLSKPAVTPK CCCCCCCCCCCCCCC	39.72	27097102
399	Acetylation	ATKSPLSKPAVTPKP CCCCCCCCCCCCCCC	44.68	-
399	Acetylation	ATKSPLSKPAVTPKP CCCCCCCCCCCCCCC	44.68	22902405
403	Phosphorylation	PLSKPAVTPKPPAAK CCCCCCCCCCCCCHH	27.92	27097102
424	Acetylation	QPAGSGQKPQSRKAD CCCCCCCCCCCCCCC	48.85	-
424	Acetylation	QPAGSGQKPQSRKAD CCCCCCCCCCCCCCC	48.85	22902405
427	Phosphorylation	GSGQKPQSRKADSSS CCCCCCCCCCCCCCC	44.25	28689409
465	Phosphorylation	VTAKAAPSLPAKQAP HCCHHCCCCCHHHCC	42.11	-
469	Acetylation	AAPSLPAKQAPRAGG HCCCCCHHHCCCCCC	45.08	-
469	Acetylation	AAPSLPAKQAPRAGG HCCCCCHHHCCCCCC	45.08	25786129
479	Phosphorylation	PRAGGDSSSDSESSS CCCCCCCCCCCCCCC	42.79	28432305
480	Phosphorylation	RAGGDSSSDSESSSS CCCCCCCCCCCCCCC	49.46	28432305
486	Phosphorylation	SSDSESSSSEEEKKT CCCCCCCCCHHHHCC	51.56	21630457
487	Phosphorylation	SDSESSSSEEEKKTP CCCCCCCCHHHHCCC	51.48	21630457
493	Phosphorylation	SSEEEKKTPPKPPAK CCHHHHCCCCCCCHH	56.76	30181290
512	Phosphorylation	GAAVPKPTPVKKAAA CCCCCCCCHHHHHHH	47.00	23984901
515	Acetylation	VPKPTPVKKAAAESS CCCCCHHHHHHHHCC	37.59	-
515	Acetylation	VPKPTPVKKAAAESS CCCCCHHHHHHHHCC	37.59	22902405
521	Phosphorylation	VKKAAAESSSSSSSS HHHHHHHCCCCCCCC	30.69	28432305
522	Phosphorylation	KKAAAESSSSSSSSE HHHHHHCCCCCCCCC	26.24	28432305
523	Phosphorylation	KAAAESSSSSSSSED HHHHHCCCCCCCCCC	43.44	28432305
524	Phosphorylation	AAAESSSSSSSSEDS HHHHCCCCCCCCCCC	35.87	28432305
525	Phosphorylation	AAESSSSSSSSEDSS HHHCCCCCCCCCCCC	35.87	28432305
526	Phosphorylation	AESSSSSSSSEDSSE HHCCCCCCCCCCCCH	39.57	28432305
527	Phosphorylation	ESSSSSSSSEDSSEE HCCCCCCCCCCCCHH	39.43	28432305
528	Phosphorylation	SSSSSSSSEDSSEEE CCCCCCCCCCCCHHH	47.58	28432305
541	Phosphorylation	EEKKKPKSKATPKPQ HHHHCCCCCCCCCCC	35.73	24972320
544	Phosphorylation	KKPKSKATPKPQAGK HCCCCCCCCCCCCCC	35.27	24972320
567	Phosphorylation	NGKAGKESEEEEEDT CCCCCCCCHHHHHHH	54.52	23712012
574	Phosphorylation	SEEEEEDTEQNKKAA CHHHHHHHHHHHHHH	42.63	29779826
587	Phosphorylation	AAGTKPGSGKKRKHN HHCCCCCCCCCCCCC	56.84	-
596	Phosphorylation	KKRKHNETADEAATP CCCCCCCCCCCCCCC	44.73	29779826
602	Phosphorylation	ETADEAATPQSKKVK CCCCCCCCCCCCCCC	28.98	26437020
605	Phosphorylation	DEAATPQSKKVKLQT CCCCCCCCCCCCCCC	35.33	23298284
606	Acetylation	EAATPQSKKVKLQTP CCCCCCCCCCCCCCC	57.23	-
606	Acetylation	EAATPQSKKVKLQTP CCCCCCCCCCCCCCC	57.23	22902405
612	Phosphorylation	SKKVKLQTPNTFPKR CCCCCCCCCCCCCCC	29.01	27097102
615	Phosphorylation	VKLQTPNTFPKRKKG CCCCCCCCCCCCCCC	42.38	27097102
627	Phosphorylation	KKGEKRASSPFRRVR CCCCCCCCCCCCHHH	42.44	12167624
628	Phosphorylation	KGEKRASSPFRRVRE CCCCCCCCCCCHHHH	27.37	12167624
648	Phosphorylation	DSRVADNSFDAKRGA CCHHHCCCCCHHCCC	25.67	27097102
668	Acetylation	ERANQVLKFTKGKSF HHHHHHHHHHCCCCC	52.73	-
668	Acetylation	ERANQVLKFTKGKSF HHHHHHHHHHCCCCC	52.73	22902405
688	Methylation	KKKRGSYRGGSISVQ CCCCCCCCCCCEEEE	44.70	-
691	Phosphorylation	RGSYRGGSISVQVNS CCCCCCCCEEEEEEE	17.95	28432305
693	Phosphorylation	SYRGGSISVQVNSVK CCCCCCEEEEEEEEE	14.27	27097102
698	Phosphorylation	SISVQVNSVKFDSE- CEEEEEEEEECCCC-	28.03	27097102
703	Phosphorylation	VNSVKFDSE------ EEEEECCCC------	48.62	23712012

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
113	S	Phosphorylation	Kinase	PRKACA	P00517	GPS
612	T	Phosphorylation	Kinase	CDK1	P06493	PSP
615	T	Phosphorylation	Kinase	CDK1	P06493	PSP
627	S	Phosphorylation	Kinase	PRKACA	P00517	GPS
627	S	Phosphorylation	Kinase	PKACA	P17612	PSP
628	S	Phosphorylation	Kinase	PRKACA	P00517	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NOLC1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NOLC1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
CEBPB_RAT	Cebpb	physical	8972203

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NOLC1_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."; Moser K., White F.M.; J. Proteome Res. 5:98-104(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-628, AND MASSSPECTROMETRY.	16396499 [Abstract]