NMDE1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NMDE1_RAT
• UniProt AC: Q00959
• Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Gene Name: Grin2a
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1464
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein .
• Protein Description: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B. [PubMed: 28384476 Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity]
• Protein Sequence: MGRLGYWTLLVLPALLVWRDPAQNAAAEKGPPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQAEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTRFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFASTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSRMDSPKRATDFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGDNMNELQTFVANRHKDNLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCSDVDRTYMKTKASSPRDKIYTIDGEKEPSFHLDPPQFVENITLPENVGFPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGSDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATREEVYQQDWSQNNALQFQKNKLRINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHASDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNTMYSTPRVLNSCSNRRVYKKMPSIESDV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
75	N-linked_Glycosylation	NVVALLMNRTDPKSL HHHHHHCCCCCHHHH	43.60	-
340	N-linked_Glycosylation	TLHQFMVNVTWDGKD EEEEEEEEEEECCCC	17.45	-
443	N-linked_Glycosylation	CRKFVKINNSTNEGM CCCEEEECCCCCCCC	30.59	-
444	N-linked_Glycosylation	RKFVKINNSTNEGMN CCEEEECCCCCCCCC	54.40	-
511	Phosphorylation	RAVMAVGSLTINEER HHHHHHCCEEECCHH	18.75	22335236
541	N-linked_Glycosylation	SVMVSRSNGTVSPSA EEEEECCCCCCCHHH	49.39	-
687	N-linked_Glycosylation	FRFGTVPNGSTERNI CCCCCCCCCCCCCCC	53.35	-
848	S-palmitoylation	FYWKLRFCFTGVCSD HHHHHHHHHHHHCCC	2.11	19874789
853	S-palmitoylation	RFCFTGVCSDRPGLL HHHHHHHCCCCCCEE	3.56	19874789
870	S-palmitoylation	ISRGIYSCIHGVHIE EHHHHHHHHHCEECC	1.17	19874789
882	Phosphorylation	HIEEKKKSPDFNLTG ECCHHCCCCCCCCCC	38.28	25403869
888	Phosphorylation	KSPDFNLTGSQSNML CCCCCCCCCCHHHHH	35.57	22673903
890	Phosphorylation	PDFNLTGSQSNMLKL CCCCCCCCHHHHHHH	25.79	25403869
892	Phosphorylation	FNLTGSQSNMLKLLR CCCCCCHHHHHHHHH	26.38	22673903
929	Phosphorylation	TDFIQRGSLIVDMVS HHHHHHCCEEEEEEC	19.67	25403869
943	Phosphorylation	SDKGNLIYSDNRSFQ CCCCCEEECCCCCCC	17.09	27097102
944	Phosphorylation	DKGNLIYSDNRSFQG CCCCEEECCCCCCCC	22.78	27097102
948	Phosphorylation	LIYSDNRSFQGKDSI EEECCCCCCCCCCCC	28.33	25403869
976	Phosphorylation	NRHKDNLSNYVFQGQ HCCCCCHHHHCCCCC	32.16	-
978	Phosphorylation	HKDNLSNYVFQGQHP CCCCHHHHCCCCCCC	10.16	-
1025	Phosphorylation	MESLRQDSLNQNPVS HHHHHHHHCCCCCCC	22.38	27097102
1059	Phosphorylation	LPEEVAHSDISETSS CCHHHHCCCCCCCCC	27.23	25403869
1062	Phosphorylation	EVAHSDISETSSRAT HHHCCCCCCCCCCCC	39.55	25403869
1064	Phosphorylation	AHSDISETSSRATCH HCCCCCCCCCCCCCC	25.80	22673903
1099	Phosphorylation	SKYPKDCSDVDRTYM HHCCCCCCCCHHHHC	51.29	-
1104	Phosphorylation	DCSDVDRTYMKTKAS CCCCCHHHHCCCCCC	24.41	-
1105	Phosphorylation	CSDVDRTYMKTKASS CCCCHHHHCCCCCCC	9.48	-
1118	Phosphorylation	SSPRDKIYTIDGEKE CCCCCEEEEECCCCC	11.90	-
1119	Phosphorylation	SPRDKIYTIDGEKEP CCCCEEEEECCCCCC	18.96	-
1127	Phosphorylation	IDGEKEPSFHLDPPQ ECCCCCCCCCCCCHH	26.79	-
1184	Phosphorylation	GLPNNDQYKLYAKHF CCCCCHHHEEEEEEE	13.06	-
1187	Phosphorylation	NNDQYKLYAKHFTLK CCHHHEEEEEEEEEC	14.95	-
1198	Phosphorylation	FTLKDKGSPHSEGSD EEECCCCCCCCCCCH	25.77	25403869
1201	Phosphorylation	KDKGSPHSEGSDRYR CCCCCCCCCCCHHHH	47.22	25403869
1204	Phosphorylation	GSPHSEGSDRYRQNS CCCCCCCCHHHHCCC	18.18	25403869
1223	Phosphorylation	SCLSNLPTYSGHFTM HHHHCCCCCCCCEEC	33.22	25403869
1224	Phosphorylation	CLSNLPTYSGHFTMR HHHCCCCCCCCEECC	15.66	25403869
1225	Phosphorylation	LSNLPTYSGHFTMRS HHCCCCCCCCEECCC	28.41	25403869
1229	Phosphorylation	PTYSGHFTMRSPFKC CCCCCCEECCCCCCC	13.15	25403869
1232	Phosphorylation	SGHFTMRSPFKCDAC CCCEECCCCCCCCEE	24.70	25403869
1235	Ubiquitination	FTMRSPFKCDACLRM EECCCCCCCCEEEEC	34.29	-
1246	Phosphorylation	CLRMGNLYDIDEDQM EEECCCEECCCHHHH	18.17	-
1262	Phosphorylation	QETGNPATREEVYQQ HHHCCCCCHHHHHHH	39.61	-
1267	Phosphorylation	PATREEVYQQDWSQN CCCHHHHHHHCCCCC	12.07	-
1291	Phosphorylation	LRINRQHSYDNILDK HHHCCCCCCCCCCCC	26.33	23625947
1292	Phosphorylation	RINRQHSYDNILDKP HHCCCCCCCCCCCCC	15.75	-
1325	Phosphorylation	RLLEGNLYGSLFSVP HHHCCCCHHHCCCCC	14.52	-
1327	Phosphorylation	LEGNLYGSLFSVPSS HCCCCHHHCCCCCHH	16.82	-
1370	Phosphorylation	SDNPFLHTYGDDQRL CCCCCCCCCCCCCEE	31.20	-
1371	Phosphorylation	DNPFLHTYGDDQRLV CCCCCCCCCCCCEEE	14.07	-
1384	Phosphorylation	LVIGRCPSDPYKHSL EEEEECCCCCCCCCC	55.64	-
1387	Phosphorylation	GRCPSDPYKHSLPSQ EECCCCCCCCCCCHH	26.73	15748156
1390	Phosphorylation	PSDPYKHSLPSQAVN CCCCCCCCCCHHHCC	37.51	-
1399	Phosphorylation	PSQAVNDSYLRSSLR CHHHCCHHHHHHHHH	22.36	-
1400	Phosphorylation	SQAVNDSYLRSSLRS HHHCCHHHHHHHHHH	14.70	-
1416	Phosphorylation	ASYCSRDSRGHSDVY HHHHCCCCCCCCCEE	38.77	22817900
1423	Phosphorylation	SRGHSDVYISEHVMP CCCCCCEEEECCCHH	12.06	-
1425	Phosphorylation	GHSDVYISEHVMPYA CCCCEEEECCCHHCC	12.17	-
1431	Phosphorylation	ISEHVMPYAANKNTM EECCCHHCCCCCCCC	10.55	-
1437	Phosphorylation	PYAANKNTMYSTPRV HCCCCCCCCCCCHHH	21.06	-
1439	Phosphorylation	AANKNTMYSTPRVLN CCCCCCCCCCHHHHH	13.83	-
1440	Phosphorylation	ANKNTMYSTPRVLNS CCCCCCCCCHHHHHH	22.55	-
1459	Phosphorylation	RVYKKMPSIESDV-- CHHHCCCCCCCCC--	35.17	22673903
1462	Phosphorylation	KKMPSIESDV----- HCCCCCCCCC-----	41.84	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1048	S	Phosphorylation	Kinase	DYRK1A	Q13627	PSP
1232	S	Phosphorylation	Kinase	CDK5	Q03114	PSP
1416	S	Phosphorylation	Kinase	PRKCA	P05696	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NMDE1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NMDE1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DLG4_RAT	Dlg4	physical	8601796
DLG1_RAT	Dlg1	physical	8601796

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.