NISCH_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NISCH_MOUSE
• UniProt AC: Q80TM9
• Protein Name: Nischarin
• Gene Name: Nisch
• Organism: Mus musculus (Mouse).
• Sequence Length: 1593
• Subcellular Localization: Cell membrane. Cytoplasm . Early endosome. Recycling endosome. Enriched in the early/sorting and recycling endosomes (By similarity). Colocalized in early/sorting endosomes with EEA1 and SNX2 and in recycling endosomes with transferrin receptor (By s
• Protein Description: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis (By similarity). Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3 (By similarity). When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures (By similarity)..
• Protein Sequence: MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKSLLPPKKIIGKNSRSLVEKRERDLEVYLQTLLTTFPDVAPRVLAHFLHFHLYEVNGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIKEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVTSKPTLATMSVRFSATSMKEVLAPEASEFDEWEPEGTATLGGPVTAIIPTWQALTTLDLSHNSICEIDESVKLIPKIEYLDLSHNGLRVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLESLSGLHKLYSLVNVDLRDNRIEQLDEVKSIGSLPCLERLTLLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSNTEKKAGEDFRLPPAPCIRPGGSPPAAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLPPEDHRPIARACSDSLESIPAGQVASDDLRDVPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEEDVAENRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTTLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTPAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVISKNPSAKPRNQPAKSRASAEQRLQETPADAPAPAAVPPTASAPAPAEALAPDLAPVQAPGEDRGLTSAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAPAEAPAAAEAPAAAEAPAAAEAPAAAEAPASAEAPAPNQAPAPARGPAPARGPAPAGGPAPAEALAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHNSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSILLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRVGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MAAATLSFGPER ---CCCCCCCCCCHH	20.43	28066266
7	Phosphorylation	-MAAATLSFGPEREA -CCCCCCCCCCHHCC	24.65	26824392
52	Phosphorylation	WTIKHRYSDFHDLHE EEEEEECCCHHHHHH	33.48	29899451
190	Phosphorylation	DFTCRLKYLKVSGTE HHEEEEEEEEECCCC	19.15	20116462
206	Ubiquitination	PFGTSNIKEQLLPFD CCCCCCHHHHHCCCC	43.59	-
238	Phosphorylation	KHIRGLVTSKPTLAT HHHHCCCCCCCCEEE	35.75	26643407
239	Phosphorylation	HIRGLVTSKPTLATM HHHCCCCCCCCEEEE	29.20	26643407
242	Phosphorylation	GLVTSKPTLATMSVR CCCCCCCCEEEEEEE	32.53	28576409
247	Phosphorylation	KPTLATMSVRFSATS CCCEEEEEEEECCCC	13.04	-
251	Phosphorylation	ATMSVRFSATSMKEV EEEEEEECCCCHHHH	21.77	-
253	Phosphorylation	MSVRFSATSMKEVLA EEEEECCCCHHHHHC	28.31	-
254	Phosphorylation	SVRFSATSMKEVLAP EEEECCCCHHHHHCC	27.81	-
264	Phosphorylation	EVLAPEASEFDEWEP HHHCCCCCCCCCCCC	36.81	-
360	Ubiquitination	HTKLGNVKTLNLAGN HHHCCCCEEEECCHH	51.82	-
425	Ubiquitination	IIPDYRTKVLSQFGE CCCCHHHHHHHHHHH	31.98	22790023
436	Phosphorylation	QFGERASEICLDDVA HHHHHHHHHCHHCCC	36.42	19144319
447	Ubiquitination	DDVATTEKELDTVEV HCCCCCHHHCHHHHH	62.58	-
492 (in isoform 6)	Phosphorylation	-	33.36	25521595
492	Phosphorylation	PCIRPGGSPPAAPAS CCCCCCCCCCCCCCC	33.36	26824392
499 (in isoform 6)	Phosphorylation	-	19.82	22324799
501 (in isoform 6)	Phosphorylation	-	37.78	22324799
543	Phosphorylation	RPIARACSDSLESIP HHHHHHHHHCHHCCC	29.29	25521595
545	Phosphorylation	IARACSDSLESIPAG HHHHHHHCHHCCCCC	20.10	25521595
548	Phosphorylation	ACSDSLESIPAGQVA HHHHCHHCCCCCCCC	38.88	25521595
556	Phosphorylation	IPAGQVASDDLRDVP CCCCCCCCCCCCCCC	32.57	25619855
669	Phosphorylation	DAEEEEGSGQGEEDE CCCCCCCCCCCCCCC	31.09	27180971
721	Phosphorylation	FLIHVQGSIRQFAAC HHHHHHCHHHHHHHH	9.48	26824392
755	Phosphorylation	GSSQHILSSLRFVFC CCHHHHHHHCEEEEE	27.07	25159016
756	Phosphorylation	SSQHILSSLRFVFCF CHHHHHHHCEEEEEE	21.81	25159016
843	Ubiquitination	RQLLTFYKVAGGSQE HHHHHHHHHHCCCCH	22.78	-
865	Ubiquitination	VYLVYSDKRMVQTPA EEEEEECCCEEECCC	36.13	22790023
870	Phosphorylation	SDKRMVQTPAGDYSG ECCCEEECCCCCCCC	12.05	27600695
902	Ubiquitination	CAPSEAVKSAAIPYW CCCHHHHHHCHHHHH	41.23	-
991	Ubiquitination	IFVLPHEKFHFLRVY EEEECCCHHHHHHHH	40.90	-
1009	Ubiquitination	RASLQDLKTVVISKN HHHHHHCCEEEEECC	47.79	22790023
1367	Phosphorylation	HLMLVLSSLERTPSP HHHHHHHHHCCCCCC	30.04	24704852
1371	Phosphorylation	VLSSLERTPSPEPVD HHHHHCCCCCCCCCC	20.81	27742792
1373	Phosphorylation	SSLERTPSPEPVDKD HHHCCCCCCCCCCHH	41.08	25521595
1379	Ubiquitination	PSPEPVDKDFYSEFG CCCCCCCHHHHHHHC	50.39	22790023
1382	Phosphorylation	EPVDKDFYSEFGDKN CCCCHHHHHHHCCCC	19.88	27742792
1383	Phosphorylation	PVDKDFYSEFGDKNT CCCHHHHHHHCCCCC	26.83	25159016
1388	Ubiquitination	FYSEFGDKNTGKMEN HHHHHCCCCCCCCCC	57.71	22790023
1392	Ubiquitination	FGDKNTGKMENYELI HCCCCCCCCCCEEEE	40.79	22790023
1396	Phosphorylation	NTGKMENYELIHSSR CCCCCCCEEEEEECC	9.98	25159016
1405	Ubiquitination	LIHSSRVKFTYPSEE EEEECCEEEECCCHH	30.77	22790023
1570	Ubiquitination	PSAESREKLISLLAR CCHHHHHHHHHHHHH	50.43	22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NISCH_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NISCH_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NISCH_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of NISCH_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.