dbPTM

NFM_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFM_MOUSE
UniProt AC	P08553
Protein Name	Neurofilament medium polypeptide
Gene Name	Nefm
Organism	Mus musculus (Mouse).
Sequence Length	848
Subcellular Localization
Protein Description	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber..
Protein Sequence	MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSMVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLECHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFSGSITGPLYTHRQPSVTISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEETLTAIAEELAASAKEEKEEAEEKEEEPEAEKSPVKSPEAKEEEEEGEKEEEEEGQEEEEEEDEGVKSDQAEEGGSEKEGSSEKDEGEQEEEEGETEAEGEGEEAEAKEEKKIEGKVEEVAVKEEIKVEKPEKAKSPMPKSPVEEVKPKPEAKAGKGEQKEEEKVEEEKKEVTKESPKEEKVEKKEEKPKDVADKKKAESPVKEKAVEEVITISKSVKVSLEKDTKEEKPQPQEKVKEKAEEEGGSEEEGSDRSPQESKKEDIAINGEVEGKEEEEQETQEKGSGREEEKGVVTNGLDVSPAEEKKGEDSSDDKVVVTKKVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQGD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSYTLDSLG ------CCCCCCCCC	27.16	-
2	Phosphorylation	------MSYTLDSLG ------CCCCCCCCC	27.16	30635358
3	Phosphorylation	-----MSYTLDSLGN -----CCCCCCCCCC	15.26	30635358
4	Phosphorylation	----MSYTLDSLGNP ----CCCCCCCCCCH	19.44	30635358
7	Phosphorylation	-MSYTLDSLGNPSAY -CCCCCCCCCCHHHH	41.09	30635358
12	Phosphorylation	LDSLGNPSAYRRVTE CCCCCCHHHHHHHHC	41.76	30635358
18	O-linked_Glycosylation	PSAYRRVTETRSSFS HHHHHHHHCCHHHCC	29.81	22517741
20	Phosphorylation	AYRRVTETRSSFSRV HHHHHHCCHHHCCCC	26.87	18779572
22	Phosphorylation	RRVTETRSSFSRVSG HHHHCCHHHCCCCCC	43.06	22324799
23	Phosphorylation	RVTETRSSFSRVSGS HHHCCHHHCCCCCCC	24.77	22324799
25	Phosphorylation	TETRSSFSRVSGSPS HCCHHHCCCCCCCCC	33.45	22324799
26	Dimethylation	ETRSSFSRVSGSPSS CCHHHCCCCCCCCCC	25.30	-
26	Methylation	ETRSSFSRVSGSPSS CCHHHCCCCCCCCCC	25.30	97770719
28	O-linked_Glycosylation	RSSFSRVSGSPSSGF HHHCCCCCCCCCCCC	32.23	161169
28	Phosphorylation	RSSFSRVSGSPSSGF HHHCCCCCCCCCCCC	32.23	22324799
30	Phosphorylation	SFSRVSGSPSSGFRS HCCCCCCCCCCCCCC	17.69	25521595
32	Phosphorylation	SRVSGSPSSGFRSQS CCCCCCCCCCCCCCC	44.80	25521595
33	O-linked_Glycosylation	RVSGSPSSGFRSQSW CCCCCCCCCCCCCCC	45.39	22517741
33	Phosphorylation	RVSGSPSSGFRSQSW CCCCCCCCCCCCCCC	45.39	25521595
37	O-linked_Glycosylation	SPSSGFRSQSWSRGS CCCCCCCCCCCCCCC	26.72	45555461
37	Phosphorylation	SPSSGFRSQSWSRGS CCCCCCCCCCCCCCC	26.72	29899451
41	Phosphorylation	GFRSQSWSRGSPSTV CCCCCCCCCCCCCCC	31.82	29899451
42	Dimethylation	FRSQSWSRGSPSTVS CCCCCCCCCCCCCCC	43.27	-
42	Methylation	FRSQSWSRGSPSTVS CCCCCCCCCCCCCCC	43.27	24129315
44	Phosphorylation	SQSWSRGSPSTVSSS CCCCCCCCCCCCCHH	17.99	25521595
46	O-linked_Glycosylation	SWSRGSPSTVSSSYK CCCCCCCCCCCHHHH	43.04	22517741
46	Phosphorylation	SWSRGSPSTVSSSYK CCCCCCCCCCCHHHH	43.04	22817900
47	O-linked_Glycosylation	WSRGSPSTVSSSYKR CCCCCCCCCCHHHHH	28.00	22517741
47	Phosphorylation	WSRGSPSTVSSSYKR CCCCCCCCCCHHHHH	28.00	25521595
49	O-linked_Glycosylation	RGSPSTVSSSYKRSA CCCCCCCCHHHHHHC	17.41	8510427
49	Phosphorylation	RGSPSTVSSSYKRSA CCCCCCCCHHHHHHC	17.41	29899451
50	O-linked_Glycosylation	GSPSTVSSSYKRSAL CCCCCCCHHHHHHCH	33.09	8509379
50	Phosphorylation	GSPSTVSSSYKRSAL CCCCCCCHHHHHHCH	33.09	-
51	O-linked_Glycosylation	SPSTVSSSYKRSALA CCCCCCHHHHHHCHH	27.45	8509395
51	Phosphorylation	SPSTVSSSYKRSALA CCCCCCHHHHHHCHH	27.45	27841257
52	Phosphorylation	PSTVSSSYKRSALAP CCCCCHHHHHHCHHH	16.62	29899451
53	Ubiquitination	STVSSSYKRSALAPR CCCCHHHHHHCHHHH	41.65	22790023
95	Phosphorylation	SGGDYKLSRSNEKEQ CCCCCCCCCCCHHHH	29.52	22324799
97	Phosphorylation	GDYKLSRSNEKEQLQ CCCCCCCCCHHHHHC	46.06	22817900
100	Ubiquitination	KLSRSNEKEQLQGLN CCCCCCHHHHHCCCH	55.82	-
125	Ubiquitination	HYLEQQNKEIEAEIQ HHHHHHHHHHHHHHH	56.59	22790023
137	Ubiquitination	EIQALRQKQASHAQL HHHHHHHHHHHHHHC	41.20	22790023
140	Phosphorylation	ALRQKQASHAQLGDA HHHHHHHHHHHCHHH	19.50	25521595
148	Nitration	HAQLGDAYDQEIREL HHHCHHHHHHHHHHH	23.93	-
166	Ubiquitination	LEMVNHEKAQVQLDS HHHHCHHHHHHHCCC	36.25	22790023
211	Phosphorylation	LRKDIEESSMVKVEL HHHHHHHCCCEEEHH	15.79	22817900
212	Phosphorylation	RKDIEESSMVKVELD HHHHHHCCCEEEHHH	30.76	25521595
221	Ubiquitination	VKVELDKKVQSLQDE EEEHHHHHHHHHHHH	45.21	22790023
224	Phosphorylation	ELDKKVQSLQDEVAF HHHHHHHHHHHHHHH	30.87	22817900
257	Ubiquitination	SHITVERKDYLKTDI HCCEEECHHHHHCCH	36.90	-
261	Ubiquitination	VERKDYLKTDISTAL EECHHHHHCCHHHHH	37.61	22790023
269	Ubiquitination	TDISTALKEIRSQLE CCHHHHHHHHHHHHH	48.94	22790023
291	Ubiquitination	HQAEEWFKCRYAKLT HHHHHHHHHHHHHHH	20.90	22790023
296	Ubiquitination	WFKCRYAKLTEAAEQ HHHHHHHHHHHHHHH	47.19	22790023
305	Ubiquitination	TEAAEQNKEAIRSAK HHHHHHCHHHHHHHH	48.49	22790023
312	Ubiquitination	KEAIRSAKEEIAEYR HHHHHHHHHHHHHHH	58.06	22790023
318	Phosphorylation	AKEEIAEYRRQLQSK HHHHHHHHHHHHHHC	11.32	29899451
324	Phosphorylation	EYRRQLQSKSIELES HHHHHHHHCCCCHHH	36.64	22817900
325	Ubiquitination	YRRQLQSKSIELESV HHHHHHHCCCCHHHH	42.36	22790023
326	Phosphorylation	RRQLQSKSIELESVR HHHHHHCCCCHHHHH	26.92	25521595
336	Acetylation	LESVRGTKESLERQL HHHHHCCHHHHHHHH	48.40	15619981
338	Phosphorylation	SVRGTKESLERQLSD HHHCCHHHHHHHHHH	36.77	22817900
344	Phosphorylation	ESLERQLSDIEERHN HHHHHHHHHHHHHHC	28.36	25521595
355	Phosphorylation	ERHNHDLSSYQDTIQ HHHCCCCHHHHHHHH	32.81	29899451
382	Phosphorylation	MARHLREYQDLLNVK HHHHHHHHHHHHCHH	10.82	25367039
401	Ubiquitination	IEIAAYRKLLEGEET HHHHHHHHHHCCCCC	44.76	22790023
411	Phosphorylation	EGEETRFSTFSGSIT CCCCCCCCCCCCEEC	25.83	29899451
412	Phosphorylation	GEETRFSTFSGSITG CCCCCCCCCCCEECC	21.07	20415495
414	O-linked_Glycosylation	ETRFSTFSGSITGPL CCCCCCCCCEECCCC	31.55	9404141
414	Phosphorylation	ETRFSTFSGSITGPL CCCCCCCCCEECCCC	31.55	22817900
416	Phosphorylation	RFSTFSGSITGPLYT CCCCCCCEECCCCEE	18.77	22817900
418	Phosphorylation	STFSGSITGPLYTHR CCCCCEECCCCEECC	34.15	20415495
423	Phosphorylation	SITGPLYTHRQPSVT EECCCCEECCCCCEE	20.47	-
428	O-linked_Glycosylation	LYTHRQPSVTISSKI CEECCCCCEEECCCC	24.45	16161031
428	Phosphorylation	LYTHRQPSVTISSKI CEECCCCCEEECCCC	24.45	25521595
430	O-linked_Glycosylation	THRQPSVTISSKIQK ECCCCCEEECCCCCC	21.34	22517741
430	Phosphorylation	THRQPSVTISSKIQK ECCCCCEEECCCCCC	21.34	27841257
432	Phosphorylation	RQPSVTISSKIQKTK CCCCEEECCCCCCCC	19.06	29899451
434	Ubiquitination	PSVTISSKIQKTKVE CCEEECCCCCCCCCC	42.22	22790023
437	Ubiquitination	TISSKIQKTKVEAPK EECCCCCCCCCCCCC	54.51	-
460	Ubiquitination	EEIIEETKVEDEKSE HHHHHHHCCCCHHHH	47.08	22790023
466	Phosphorylation	TKVEDEKSEMEETLT HCCCCHHHHHHHHHH	39.88	29899451
473	Phosphorylation	SEMEETLTAIAEELA HHHHHHHHHHHHHHH	24.36	29899451
482	Phosphorylation	IAEELAASAKEEKEE HHHHHHHHHHHHHHH	33.98	29899451
484	Ubiquitination	EELAASAKEEKEEAE HHHHHHHHHHHHHHH	65.00	22790023
502	Phosphorylation	EEPEAEKSPVKSPEA CCCHHHCCCCCCHHH	26.82	25521595
506	Phosphorylation	AEKSPVKSPEAKEEE HHCCCCCCHHHHHHH	28.36	25521595
537	Phosphorylation	EEDEGVKSDQAEEGG HHHCCCCCHHHHCCC	32.33	25521595
545	Phosphorylation	DQAEEGGSEKEGSSE HHHHCCCCCCCCCCC	57.25	25521595
550	Phosphorylation	GGSEKEGSSEKDEGE CCCCCCCCCCCCCCC	36.09	25521595
551	Phosphorylation	GSEKEGSSEKDEGEQ CCCCCCCCCCCCCCC	60.57	25521595
565	Phosphorylation	QEEEEGETEAEGEGE CCHHCCCCCCCCCCH	51.88	25521595
592	Ubiquitination	KVEEVAVKEEIKVEK CEEEEEEECCCCCCC	39.63	22790023
605	Phosphorylation	EKPEKAKSPMPKSPV CCCHHCCCCCCCCCH	31.15	25521595
610	Phosphorylation	AKSPMPKSPVEEVKP CCCCCCCCCHHHCCC	28.45	25521595
642	Phosphorylation	EEEKKEVTKESPKEE HHHHHHHHCCCHHHH	30.13	22817900
645	Phosphorylation	KKEVTKESPKEEKVE HHHHHCCCHHHHHCC	42.71	25521595
669	Phosphorylation	ADKKKAESPVKEKAV HCHHHCCCCCHHHHH	39.59	25521595
674	Ubiquitination	AESPVKEKAVEEVIT CCCCCHHHHHHHHHH	52.60	22790023
681	Phosphorylation	KAVEEVITISKSVKV HHHHHHHHHHHHHEE	25.11	22817900
683	Phosphorylation	VEEVITISKSVKVSL HHHHHHHHHHHEEEE	15.38	22817900
684	Ubiquitination	EEVITISKSVKVSLE HHHHHHHHHHEEEEC	56.25	22790023
687	Ubiquitination	ITISKSVKVSLEKDT HHHHHHHEEEECCCC	32.95	22790023
689	Phosphorylation	ISKSVKVSLEKDTKE HHHHHEEEECCCCCC	25.70	22817900
692	Ubiquitination	SVKVSLEKDTKEEKP HHEEEECCCCCCCCC	75.81	-
695	Ubiquitination	VSLEKDTKEEKPQPQ EEECCCCCCCCCCCH	74.20	-
698	Ubiquitination	EKDTKEEKPQPQEKV CCCCCCCCCCCHHHH	50.47	-
715	Phosphorylation	KAEEEGGSEEEGSDR HHHHCCCCCCCCCCC	53.33	25521595
720	Phosphorylation	GGSEEEGSDRSPQES CCCCCCCCCCCCCHH	32.88	25521595
723	Phosphorylation	EEEGSDRSPQESKKE CCCCCCCCCCHHHHH	35.61	25521595
727	Phosphorylation	SDRSPQESKKEDIAI CCCCCCHHHHHHCCC	43.72	25521595
748	Phosphorylation	KEEEEQETQEKGSGR CHHHHHHHHHCCCCC	41.54	29514104
753	Phosphorylation	QETQEKGSGREEEKG HHHHHCCCCCHHHCC	45.83	-
763	Phosphorylation	EEEKGVVTNGLDVSP HHHCCEEECCCCCCH	22.59	22817900
769	Phosphorylation	VTNGLDVSPAEEKKG EECCCCCCHHHHHCC	20.33	25521595
779	Phosphorylation	EEKKGEDSSDDKVVV HHHCCCCCCCCCEEE	31.22	30372032
780	Phosphorylation	EKKGEDSSDDKVVVT HHCCCCCCCCCEEEE	63.25	21930439
787	Phosphorylation	SDDKVVVTKKVEKIT CCCCEEEEEEEEECC	17.34	20415495
788	Ubiquitination	DDKVVVTKKVEKITS CCCEEEEEEEEECCC	43.12	-
789	Ubiquitination	DKVVVTKKVEKITSE CCEEEEEEEEECCCC	46.38	-
792	Ubiquitination	VVTKKVEKITSEGGD EEEEEEEECCCCCCC	55.03	-
795	Phosphorylation	KKVEKITSEGGDGAT EEEEECCCCCCCCCC	37.40	27841257
802	Phosphorylation	SEGGDGATKYITKSV CCCCCCCCEEEEEEE	29.88	22817900
803	Ubiquitination	EGGDGATKYITKSVT CCCCCCCEEEEEEEE	33.84	22790023
807	Ubiquitination	GATKYITKSVTVTQK CCCEEEEEEEEEEEE	33.11	22790023
808	Phosphorylation	ATKYITKSVTVTQKV CCEEEEEEEEEEEEH	17.86	29899451
842	Ubiquitination	VTSHAIVKEVTQGD- CCCCHHHEEHHCCC-	40.11	-
845	O-linked_Glycosylation	HAIVKEVTQGD---- CHHHEEHHCCC----	28.11	22517741
845	Phosphorylation	HAIVKEVTQGD---- CHHHEEHHCCC----	28.11	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
22	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
22	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
23	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
23	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
23	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
25	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
28	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
28	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
32	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
32	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFM_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFM_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of NFM_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFM_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502; SER-610 ANDSER-645, AND MASS SPECTROMETRY.	19367708 [Abstract]
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-769, AND MASSSPECTROMETRY.	17114649 [Abstract]
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-605; SER-610; THR-642;SER-669 AND SER-715, AND MASS SPECTROMETRY.	16452087 [Abstract]
"Proteomic analysis of in vivo phosphorylated synaptic proteins."; Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I.,Blackstock W.P., Choudhary J.S., Grant S.G.; J. Biol. Chem. 280:5972-5982(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502 AND SER-506, ANDMASS SPECTROMETRY.	15572359 [Abstract]
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-318, AND MASSSPECTROMETRY.	18034455 [Abstract]