NFIL3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NFIL3_MOUSE
• UniProt AC: O08750
• Protein Name: Nuclear factor interleukin-3-regulated protein
• Gene Name: Nfil3
• Organism: Mus musculus (Mouse).
• Sequence Length: 462
• Subcellular Localization: Nucleus .
• Protein Description: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites (By similarity). Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells (By similarity). Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death..
• Protein Sequence: MQLRKMQTIKKEPAPLDPTSSSDKMLLLNSALAEVAEDLASGEDLLLNEGSMGKNKSSACRRKREFIPDEKKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEIQKLSNSTAVYFQDYQTSKAAVSSFVDEHEPAMVAGSCISVIKHSPQSSLSDVSEVSSVEHTQESPAQGGCRSPENKFPVIKQEPVELESFAREAREERGTYSTSIYQSYMGSSFSTYSHSPPLLQVHGSTSNSPRTSEADEGVVGKSSDGEDEQQVPKGPIHSPVELQRVHATVVKVPEVNPSALPHKLRIKAKAMQVKVEALDSEFEGMQKLSSPADAIAKRHFDLEKHGTSGMAHSSLPPFSVQVTNIQDWSLKSEHWHHKELSSKTQSSFKTGVVEVKDGGYKVSEAENLYLKQGIANLSAEVVSLKRFIATQPISASDSR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
130	Phosphorylation	KLKFGLISSTAYAQE HHHHCCCCCHHHHHH	27.03	22817900
132	Phosphorylation	KFGLISSTAYAQEIQ HHCCCCCHHHHHHHH	19.52	19854140
182	Phosphorylation	CISVIKHSPQSSLSD HHHEECCCCCCCCCC	21.45	25338131
191	Phosphorylation	QSSLSDVSEVSSVEH CCCCCCCHHCCCCEE	36.95	26643407
194	Phosphorylation	LSDVSEVSSVEHTQE CCCCHHCCCCEECCC	25.31	26643407
195	Phosphorylation	SDVSEVSSVEHTQES CCCHHCCCCEECCCC	36.38	26643407
199	Phosphorylation	EVSSVEHTQESPAQG HCCCCEECCCCCCCC	22.11	26643407
202	Phosphorylation	SVEHTQESPAQGGCR CCEECCCCCCCCCCC	18.73	26643407
210	Phosphorylation	PAQGGCRSPENKFPV CCCCCCCCCCCCCCC	40.41	26643407
250	Phosphorylation	IYQSYMGSSFSTYSH HHHHHCCCCCCCCCC	16.22	-
251	Phosphorylation	YQSYMGSSFSTYSHS HHHHCCCCCCCCCCC	19.93	-
274	Phosphorylation	STSNSPRTSEADEGV CCCCCCCCCCCCCCC	34.01	25619855
275	Phosphorylation	TSNSPRTSEADEGVV CCCCCCCCCCCCCCC	31.87	25619855
285	Phosphorylation	DEGVVGKSSDGEDEQ CCCCCCCCCCCCCCC	27.94	23375375
286	Phosphorylation	EGVVGKSSDGEDEQQ CCCCCCCCCCCCCCC	53.79	25521595
301	Phosphorylation	VPKGPIHSPVELQRV CCCCCCCCCCCCEEE	31.21	26824392
352	Phosphorylation	FEGMQKLSSPADAIA CCHHHHCCCHHHHHH	41.11	29472430
353	Phosphorylation	EGMQKLSSPADAIAK CHHHHCCCHHHHHHH	34.25	26824392
446	Phosphorylation	NLSAEVVSLKRFIAT HCCHHEEEHHHHHHC	33.09	-
453	Phosphorylation	SLKRFIATQPISASD EHHHHHHCCCCCCCC	30.02	29472430
457	Phosphorylation	FIATQPISASDSR-- HHHCCCCCCCCCC--	28.53	29472430
459	Phosphorylation	ATQPISASDSR---- HCCCCCCCCCC----	29.36	29472430
461	Phosphorylation	QPISASDSR------ CCCCCCCCC------	37.08	29472430

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NFIL3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NFIL3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NFIL3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SRBP1_MOUSE	Srebf1	physical	27252523

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286, AND MASSSPECTROMETRY.
PubMed: 17242355