dbPTM

NFAC3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFAC3_HUMAN
UniProt AC	Q12968
Protein Name	Nuclear factor of activated T-cells, cytoplasmic 3
Gene Name	NFATC3
Organism	Homo sapiens (Human).
Sequence Length	1075
Subcellular Localization	Cytoplasm . Nucleus . Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sus
Protein Description	Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2..
Protein Sequence	MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSPCGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSDDQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETEGSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESKIIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPVPHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVVYNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVADQITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPSSLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDVNEIIGRDMSQISVSQGAGVSRQAPLPSPESLDLGRSDGL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MTTANCGAH ------CCCCCCCCC	46.01	-
82	Phosphorylation	SPSFQLQSHKNYEGT CCCEECCCCCCCCCE	45.05	-
86	Phosphorylation	QLQSHKNYEGTCEIP ECCCCCCCCCEEECC	22.12	28796482
89	Phosphorylation	SHKNYEGTCEIPESK CCCCCCCEEECCHHH	8.60	30108239
95	Phosphorylation	GTCEIPESKYSPLGG CEEECCHHHCCCCCC	31.38	30108239
96	Ubiquitination	TCEIPESKYSPLGGP EEECCHHHCCCCCCC	48.35	-
97	Phosphorylation	CEIPESKYSPLGGPK EECCHHHCCCCCCCC	25.46	30576142
98	Phosphorylation	EIPESKYSPLGGPKP ECCHHHCCCCCCCCC	19.86	25849741
110	Phosphorylation	PKPFECPSIQITSIS CCCCCCCCEEEEEEC	38.49	26074081
114	Phosphorylation	ECPSIQITSISPNCH CCCCEEEEEECCCHH	12.02	26074081
115	Phosphorylation	CPSIQITSISPNCHQ CCCEEEEEECCCHHC	23.77	26074081
117	Phosphorylation	SIQITSISPNCHQEL CEEEEEECCCHHCCH	15.16	25159151
145	Phosphorylation	REFLERPSRDHLYLP HHHHHCCCCCCEEEE	57.47	-
150	Phosphorylation	RPSRDHLYLPLEPSY CCCCCCEEEECCCCC	11.67	28796482
156	Phosphorylation	LYLPLEPSYRESSLS EEEECCCCCCCCCCC	27.70	28122231
160	Phosphorylation	LEPSYRESSLSPSPA CCCCCCCCCCCCCCC	27.81	28450419
161	Phosphorylation	EPSYRESSLSPSPAS CCCCCCCCCCCCCCC	28.28	28450419
163	Phosphorylation	SYRESSLSPSPASSI CCCCCCCCCCCCCCC	25.86	25159151
165	Phosphorylation	RESSLSPSPASSISS CCCCCCCCCCCCCCC	29.71	19690332
168	Phosphorylation	SLSPSPASSISSRSW CCCCCCCCCCCCCCC	31.43	25262027
169	Phosphorylation	LSPSPASSISSRSWF CCCCCCCCCCCCCCC	28.70	25262027
172	Phosphorylation	SPASSISSRSWFSDA CCCCCCCCCCCCCCC	28.76	-
177	Phosphorylation	ISSRSWFSDASSCES CCCCCCCCCCHHCHH	26.47	22817900
180	Phosphorylation	RSWFSDASSCESLSH CCCCCCCHHCHHHHH	40.28	22817900
181	Phosphorylation	SWFSDASSCESLSHI CCCCCCHHCHHHHHH	24.51	22817900
184	Phosphorylation	SDASSCESLSHIYDD CCCHHCHHHHHHHHC	39.23	22817900
186	Phosphorylation	ASSCESLSHIYDDVD CHHCHHHHHHHHCCC	19.67	22817900
189	Phosphorylation	CESLSHIYDDVDSEL CHHHHHHHHCCCHHH	10.73	27642862
204	Phosphorylation	NEAAARFTLGSPLTS HHHHHHHCCCCCCCC	25.34	20873877
207	Phosphorylation	AARFTLGSPLTSPGG HHHHCCCCCCCCCCC	21.61	20873877
210	Phosphorylation	FTLGSPLTSPGGSPG HCCCCCCCCCCCCCC	35.94	20873877
211	Phosphorylation	TLGSPLTSPGGSPGG CCCCCCCCCCCCCCC	29.27	20873877
215	Phosphorylation	PLTSPGGSPGGCPGE CCCCCCCCCCCCCCC	26.70	20873877
224	Phosphorylation	GGCPGEETWHQQYGL CCCCCCCHHHHHCCC	24.76	20873877
229	Phosphorylation	EETWHQQYGLGHSLS CCHHHHHCCCCCCCC	14.03	20873877
234	Phosphorylation	QQYGLGHSLSPRQSP HHCCCCCCCCCCCCC	28.83	20873877
236	Phosphorylation	YGLGHSLSPRQSPCH CCCCCCCCCCCCCCC	22.50	20873877
240	Phosphorylation	HSLSPRQSPCHSPRS CCCCCCCCCCCCCCC	30.67	26074081
244	Phosphorylation	PRQSPCHSPRSSVTD CCCCCCCCCCCCCCC	28.82	26074081
247	Phosphorylation	SPCHSPRSSVTDENW CCCCCCCCCCCCCCC	32.32	23401153
248	Phosphorylation	PCHSPRSSVTDENWL CCCCCCCCCCCCCCC	30.25	27794612
250	Phosphorylation	HSPRSSVTDENWLSP CCCCCCCCCCCCCCC	39.27	30576142
256	Phosphorylation	VTDENWLSPRPASGP CCCCCCCCCCCCCCC	15.52	30576142
261	Phosphorylation	WLSPRPASGPSSRPT CCCCCCCCCCCCCCC	54.65	30576142
264	Phosphorylation	PRPASGPSSRPTSPC CCCCCCCCCCCCCCC	42.37	30576142
265	Phosphorylation	RPASGPSSRPTSPCG CCCCCCCCCCCCCCC	45.47	30576142
268	Phosphorylation	SGPSSRPTSPCGKRR CCCCCCCCCCCCCCC	44.16	23401153
269	Phosphorylation	GPSSRPTSPCGKRRH CCCCCCCCCCCCCCC	22.37	23401153
277	Phosphorylation	PCGKRRHSSAEVCYA CCCCCCCCCCCEEEC	29.45	28450419
278	Phosphorylation	CGKRRHSSAEVCYAG CCCCCCCCCCEEECC	23.48	27080861
283	Phosphorylation	HSSAEVCYAGSLSPH CCCCCEEECCCCCCC	21.12	28450419
286	Phosphorylation	AEVCYAGSLSPHHSP CCEEECCCCCCCCCC	19.94	28450419
288	Phosphorylation	VCYAGSLSPHHSPVP EEECCCCCCCCCCCC	24.72	28450419
292	Phosphorylation	GSLSPHHSPVPSPGH CCCCCCCCCCCCCCC	25.26	28450419
296	Phosphorylation	PHHSPVPSPGHSPRG CCCCCCCCCCCCCCC	43.69	28450419
300	Phosphorylation	PVPSPGHSPRGSVTE CCCCCCCCCCCCCCC	23.39	28450419
304	Phosphorylation	PGHSPRGSVTEDTWL CCCCCCCCCCCCCEE	27.44	26074081
306	Phosphorylation	HSPRGSVTEDTWLNA CCCCCCCCCCCEEEE	29.82	26074081
334	Phosphorylation	PFQYCVETDIPLKTR CCEEEEECCCCCCCC	20.87	26074081
340	Phosphorylation	ETDIPLKTRKTSEDQ ECCCCCCCCCCCHHH	45.11	26074081
342	Ubiquitination	DIPLKTRKTSEDQAA CCCCCCCCCCHHHHH	62.73	-
343	Phosphorylation	IPLKTRKTSEDQAAI CCCCCCCCCHHHHHH	33.77	29255136
344	Phosphorylation	PLKTRKTSEDQAAIL CCCCCCCCHHHHHHC	42.06	29255136
354	Ubiquitination	QAAILPGKLELCSDD HHHHCCCEEEECCCC	36.53	-
359	Phosphorylation	PGKLELCSDDQGSLS CCEEEECCCCCCCCC	57.77	23927012
364	Phosphorylation	LCSDDQGSLSPARET ECCCCCCCCCCCCCC	21.75	23927012
366	Phosphorylation	SDDQGSLSPARETSI CCCCCCCCCCCCCCC	20.43	19664994
371	Phosphorylation	SLSPARETSIDDGLG CCCCCCCCCCCCCCC	25.98	30576142
372	Phosphorylation	LSPARETSIDDGLGS CCCCCCCCCCCCCCC	21.19	30576142
384	Ubiquitination	LGSQYPLKKDSCGDQ CCCCCCCCCCCCCCC	50.45	-
397	Phosphorylation	DQFLSVPSPFTWSKP CCCCCCCCCCCCCCC	30.41	26074081
400	Phosphorylation	LSVPSPFTWSKPKPG CCCCCCCCCCCCCCC	32.01	26074081
402	Phosphorylation	VPSPFTWSKPKPGHT CCCCCCCCCCCCCCC	35.70	28787133
403	Acetylation	PSPFTWSKPKPGHTP CCCCCCCCCCCCCCC	48.93	25953088
403	Ubiquitination	PSPFTWSKPKPGHTP CCCCCCCCCCCCCCC	48.93	-
405	Ubiquitination	PFTWSKPKPGHTPIF CCCCCCCCCCCCCCC	68.33	-
409	Phosphorylation	SKPKPGHTPIFRTSS CCCCCCCCCCCCCCC	24.80	29514088
414	Phosphorylation	GHTPIFRTSSLPPLD CCCCCCCCCCCCCCC	16.36	23663014
415	Phosphorylation	HTPIFRTSSLPPLDW CCCCCCCCCCCCCCC	26.14	23898821
416	Phosphorylation	TPIFRTSSLPPLDWP CCCCCCCCCCCCCCC	44.26	25159151
452	Phosphorylation	AHYETEGSRGAVKAS EEEECCCCCCCEECC	22.70	23403867
457	Ubiquitination	EGSRGAVKASTGGHP CCCCCCEECCCCCCC	35.76	-
459	Phosphorylation	SRGAVKASTGGHPVV CCCCEECCCCCCCEE	23.13	23312004
460	Phosphorylation	RGAVKASTGGHPVVK CCCEECCCCCCCEEE	52.63	28555341
467	Ubiquitination	TGGHPVVKLLGYNEK CCCCCEEEECCCCCC	37.92	-
517	Phosphorylation	SQEIIIASTKVLEIP CCEEEEEECEEEECC	20.53	23403867
518	Phosphorylation	QEIIIASTKVLEIPL CEEEEEECEEEECCC	19.02	23403867
532	Phosphorylation	LLPENNMSASIDCAG CCCCCCCCCEEEHHH	22.46	23403867
534	Phosphorylation	PENNMSASIDCAGIL CCCCCCCEEEHHHHH	16.20	23403867
542	Ubiquitination	IDCAGILKLRNSDIE EEHHHHHHHHCCCCE	43.40	-
546	Phosphorylation	GILKLRNSDIELRKG HHHHHHCCCCEECCC	33.27	28450419
552	Ubiquitination	NSDIELRKGETDIGR CCCCEECCCCCCCCC	73.96	-
630	Ubiquitination	SNFLPESKIIFLEKG CCCCCCCEEEEEECC	37.85	-
636	Ubiquitination	SKIIFLEKGQDGRPQ CEEEEEECCCCCCCE	65.11	-
636 (in isoform 5)	Ubiquitination	-	65.11	-
649	Ubiquitination	PQWEVEGKIIREKCQ CEEEEECEEHHHHCC	22.81	-
649 (in isoform 5)	Ubiquitination	-	22.81	-
690	Phosphorylation	CNGKRKKSQSQRFTY ECCCCCCCHHCCCCC	37.79	29759185
692	Phosphorylation	GKRKKSQSQRFTYTP CCCCCCHHCCCCCCC	30.34	29759185
697	Phosphorylation	SQSQRFTYTPVLMKQ CHHCCCCCCCEECCH	13.31	29759185
698	Phosphorylation	QSQRFTYTPVLMKQE HHCCCCCCCEECCHH	12.12	29759185
730	Phosphorylation	PAQTQRPSSDSGCSH CCCCCCCCCCCCCCC	49.30	20044836
731	Phosphorylation	AQTQRPSSDSGCSHD CCCCCCCCCCCCCCH	38.18	20044836
742	Phosphorylation	CSHDSVLSGQRSLIC CCCHHHHCCCCEEEE	30.62	20044836
754	Phosphorylation	LICSIPQTYASMVTS EEEECCHHHHHHHHH	18.72	28796482
755	Phosphorylation	ICSIPQTYASMVTSS EEECCHHHHHHHHHC	7.45	28796482
757	Phosphorylation	SIPQTYASMVTSSHL ECCHHHHHHHHHCCC	11.90	28796482
884	Phosphorylation	PHLQSMGYHCSNTGQ HCHHHCCCCCCCCCC	7.33	-
1045	Phosphorylation	EIIGRDMSQISVSQG HHHCCCHHHCEECCC	28.75	28555341
1048	Phosphorylation	GRDMSQISVSQGAGV CCCHHHCEECCCCCC	14.21	28555341
1063	Phosphorylation	SRQAPLPSPESLDLG CCCCCCCCHHHHCCC	49.32	30266825
1066	Phosphorylation	APLPSPESLDLGRSD CCCCCHHHHCCCCCC	30.48	30266825
1072	Phosphorylation	ESLDLGRSDGL---- HHHCCCCCCCC----	33.94	24732914

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
163	S	Phosphorylation	Kinase	MAPK9	P45984	GPS
165	S	Phosphorylation	Kinase	MAPK9	P45984	GPS
165	S	Phosphorylation	Kinase	CSNK1A1	P97633	GPS
177	S	Phosphorylation	Kinase	CSNK1A1	P97633	GPS
180	S	Phosphorylation	Kinase	CSNK1A1	P97633	GPS
181	S	Phosphorylation	Kinase	CSNK1A1	P97633	GPS
184	S	Phosphorylation	Kinase	CSNK1A1	P97633	GPS
186	S	Phosphorylation	Kinase	CSNK1A1	P97633	GPS
204	T	Phosphorylation	Kinase	CK1_GROUP	-	PhosphoELM
204	T	Phosphorylation	Kinase	CK1-FAMILY	-	GPS
204	T	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
207	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
207	S	Phosphorylation	Kinase	CK1_GROUP	-	PhosphoELM
207	S	Phosphorylation	Kinase	CK1-FAMILY	-	GPS
210	T	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
210	T	Phosphorylation	Kinase	CK1_GROUP	-	PhosphoELM
210	T	Phosphorylation	Kinase	CK1-FAMILY	-	GPS
211	S	Phosphorylation	Kinase	CK1_GROUP	-	PhosphoELM
211	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
211	S	Phosphorylation	Kinase	CK1-FAMILY	-	GPS
215	S	Phosphorylation	Kinase	CK1-FAMILY	-	GPS
215	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
215	S	Phosphorylation	Kinase	CK1_GROUP	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFAC3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFAC3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KC1A_HUMAN	CSNK1A1	physical	26186194
SUFU_HUMAN	SUFU	physical	26186194
SUFU_HUMAN	SUFU	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFAC3_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, AND MASSSPECTROMETRY.	19690332 [Abstract]