NEUG_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NEUG_RAT
• UniProt AC: Q04940
• Protein Name: Neurogranin
• Gene Name: Nrgn
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 78
• Subcellular Localization: Cytoplasm . Cell junction, synapse . Cell projection, dendritic spine . Restricted to dendritic spines of a subset of neurons.
• Protein Description: Regulates the affinity of calmodulin for calcium. Involved in synaptic plasticity and spatial learning (By similarity)..
• Protein Sequence: MDCCTESACSKPDDDILDIPLDDPGANAAAAKIQASFRGHMARKKIKSGECGRKGPGPGGPGGAGGARGGAGGGPSGD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDCCTESA -------CCCCCHHH	8.86	-
3	Glutathionylation	-----MDCCTESACS -----CCCCCHHHCC	2.74	11060308
4	Glutathionylation	----MDCCTESACSK ----CCCCCHHHCCC	4.05	11060308
9	Glutathionylation	DCCTESACSKPDDDI CCCCHHHCCCCCCCC	8.17	11060308
36	Phosphorylation	AAAKIQASFRGHMAR HHHHHHHHHHHHHCH	10.09	30411139
51	Glutathionylation	KKIKSGECGRKGPGP HHCCCCCCCCCCCCC	7.93	11060308
54	Ubiquitination	KSGECGRKGPGPGGP CCCCCCCCCCCCCCC	55.53	-
68	Citrullination	PGGAGGARGGAGGGP CCCCCCCCCCCCCCC	47.56	-
68	Citrullination	PGGAGGARGGAGGGP CCCCCCCCCCCCCCC	47.56	-
68	Methylation	PGGAGGARGGAGGGP CCCCCCCCCCCCCCC	47.56	-
76	Phosphorylation	GGAGGGPSGD----- CCCCCCCCCC-----	59.89	28551015

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
36	S	Phosphorylation	Kinase	PHK-FAMILY	-	GPS
36	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
36	S	Phosphorylation	Kinase	PHK	-	Uniprot
36	S	Phosphorylation	Kinase	PKC	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
36	S	Phosphorylation	Phosphorylated at Ser-36 by PHK and PKC.	8080473

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NEUG_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphorylase kinase phosphorylates the calmodulin-binding regulatoryregions of neuronal tissue-specific proteins B-50 (GAP-43) andneurogranin.";
Paudel H.K., Zwiers H., Wang J.H.;
J. Biol. Chem. 268:6207-6213(1993).
Cited for: PHOSPHORYLATION AT SER-36 BY PHK.
PubMed: 8454596

"Characterization of a 7.5-kDa protein kinase C substrate (RC3protein, neurogranin) from rat brain.";
Huang K.-P., Huang F.L., Chen H.-C.;
Arch. Biochem. Biophys. 305:570-580(1993).
Cited for: PROTEIN SEQUENCE, PHOSPHORYLATION AT SER-36, AND INTERACTION WITHCALM1.
PubMed: 8080473