NEP1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NEP1_MOUSE
• UniProt AC: O35130
• Protein Name: Ribosomal RNA small subunit methyltransferase NEP1 {ECO:0000250|UniProtKB:Q92979}
• Gene Name: Emg1 {ECO:0000250|UniProtKB:Q92979}
• Organism: Mus musculus (Mouse).
• Sequence Length: 244
• Subcellular Localization: Nucleus, nucleolus .
• Protein Description: S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes..
• Protein Sequence: MSAASGGFQPRERRFSVQEQDWETTPPKKLRLGAGSKCGGRRLIVVLEGASLETVKVGKTYELLNCDRHKSMLLKNGRDPGEVRPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKIGTSFSVEDISDIRELVPSSDPVVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKVTTAFEEVWGVI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSAASGGFQ ------CCCCCCCCC	32.32	28066266
5	Phosphorylation	---MSAASGGFQPRE ---CCCCCCCCCCCC	38.88	28066266
16	Phosphorylation	QPRERRFSVQEQDWE CCCCCCCCCCCCCCC	22.53	27087446
24	Phosphorylation	VQEQDWETTPPKKLR CCCCCCCCCCCCEEE	40.11	23684622
25	Phosphorylation	QEQDWETTPPKKLRL CCCCCCCCCCCEEEE	26.53	23684622
36	Phosphorylation	KLRLGAGSKCGGRRL EEEECCCCCCCCEEE	25.00	29514104
59	Ubiquitination	LETVKVGKTYELLNC EEEEECCCEEEECCC	51.42	22790023
60	Phosphorylation	ETVKVGKTYELLNCD EEEECCCEEEECCCC	19.12	25367039
61	Phosphorylation	TVKVGKTYELLNCDR EEECCCEEEECCCCH	14.20	22817900
145	Phosphorylation	VQLLHKLSVRAADGP HHHHHHHHHHCCCCH	17.85	22942356
154	Ubiquitination	RAADGPQKLLKVIKN HCCCCHHHHHHHHCC	59.94	22790023
160	Ubiquitination	QKLLKVIKNPVSDHF HHHHHHHCCCCCCCC	59.18	22790023
176	Phosphorylation	VGCMKIGTSFSVEDI CCCEEECCCEEEECH	30.03	28066266
177	Phosphorylation	GCMKIGTSFSVEDIS CCEEECCCEEEECHH	15.43	28066266
179	Phosphorylation	MKIGTSFSVEDISDI EEECCCEEEECHHHH	25.10	28066266
184	Phosphorylation	SFSVEDISDIRELVP CEEEECHHHHHHHCC	39.03	28066266
222	Phosphorylation	KMVSISNYPLSAALT EEEEECCCCHHHHHH	9.96	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NEP1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NEP1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NEP1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of NEP1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, AND MASSSPECTROMETRY.
PubMed: 17242355