NDKB_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NDKB_MOUSE
• UniProt AC: Q01768
• Protein Name: Nucleoside diphosphate kinase B
• Gene Name: Nme2
• Organism: Mus musculus (Mouse).
• Sequence Length: 152
• Subcellular Localization: Cytoplasm . Nucleus . Cell projection, lamellipodium . Cell projection, ruffle . Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vi
• Protein Description: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC. Exhibits histidine protein kinase activity (By similarity)..
• Protein Sequence: MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIHLWFKPEELIDYKSCAHDWVYE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12	Ubiquitination	ERTFIAIKPDGVQRG EEEEEEECCCCHHHC	28.21	-
26	Acetylation	GLVGEIIKRFEQKGF CHHHHHHHHHHHCCH	57.61	8275721
26	Ubiquitination	GLVGEIIKRFEQKGF CHHHHHHHHHHHCCH	57.61	-
31	Acetylation	IIKRFEQKGFRLVAM HHHHHHHCCHHHHHH	53.47	129825
39	Ubiquitination	GFRLVAMKFLRASEE CHHHHHHHHHHHCHH	31.35	-
39	Acetylation	GFRLVAMKFLRASEE CHHHHHHHHHHHCHH	31.35	22826441
44	Phosphorylation	AMKFLRASEEHLKQH HHHHHHHCHHHHHHH	36.76	28507225
49	Malonylation	RASEEHLKQHYIDLK HHCHHHHHHHCCCCC	36.62	26320211
49	Ubiquitination	RASEEHLKQHYIDLK HHCHHHHHHHCCCCC	36.62	-
49	Acetylation	RASEEHLKQHYIDLK HHCHHHHHHHCCCCC	36.62	22826441
52	Phosphorylation	EEHLKQHYIDLKDRP HHHHHHHCCCCCCCC	8.14	22499769
56	Ubiquitination	KQHYIDLKDRPFFPG HHHCCCCCCCCCCCC	48.20	-
56	Acetylation	KQHYIDLKDRPFFPG HHHCCCCCCCCCCCC	48.20	23201123
66	Acetylation	PFFPGLVKYMNSGPV CCCCCHHHHCCCCCE	43.77	30585625
70	Phosphorylation	GLVKYMNSGPVVAMV CHHHHCCCCCEEEEE	28.13	26643407
85	Acetylation	WEGLNVVKTGRVMLG ECCCCEEEECCEEEC	40.76	22638507
85	Malonylation	WEGLNVVKTGRVMLG ECCCCEEEECCEEEC	40.76	26073543
94	Phosphorylation	GRVMLGETNPADSKP CCEEECCCCCCCCCC	44.71	26824392
99	Phosphorylation	GETNPADSKPGTIRG CCCCCCCCCCCCCCC	42.74	23984901
100	Ubiquitination	ETNPADSKPGTIRGD CCCCCCCCCCCCCCC	48.27	-
100	Acetylation	ETNPADSKPGTIRGD CCCCCCCCCCCCCCC	48.27	7618953
100	Malonylation	ETNPADSKPGTIRGD CCCCCCCCCCCCCCC	48.27	26073543
103	Phosphorylation	PADSKPGTIRGDFCI CCCCCCCCCCCCEEE	18.94	24068923
109	S-nitrosylation	GTIRGDFCIQVGRNI CCCCCCEEEEECCCE	2.24	24895380
109	S-palmitoylation	GTIRGDFCIQVGRNI CCCCCCEEEEECCCE	2.24	28526873
120	Phosphorylation	GRNIIHGSDSVESAE CCCEECCCCCHHHHH	16.73	25521595
122	Phosphorylation	NIIHGSDSVESAEKE CEECCCCCHHHHHHE	29.49	27087446
125	Phosphorylation	HGSDSVESAEKEIHL CCCCCHHHHHHEEEE	39.30	26824392
128	Acetylation	DSVESAEKEIHLWFK CCHHHHHHEEEEEEC	62.78	23954790
135	Acetylation	KEIHLWFKPEELIDY HEEEEEECHHHHCCC	39.68	23954790
151	Phosphorylation	SCAHDWVYE------ CCCCCCCCC------	16.21	22499769

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NDKB_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NDKB_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NDKB_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of NDKB_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-94, AND MASSSPECTROMETRY.
PubMed: 19367708