NASP_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NASP_MOUSE
• UniProt AC: Q99MD9
• Protein Name: Nuclear autoantigenic sperm protein
• Gene Name: Nasp {ECO:0000312|MGI:MGI:1355328}
• Organism: Mus musculus (Mouse).
• Sequence Length: 773
• Subcellular Localization: Cytoplasm . Nucleus .
• Protein Description: Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and linker H1 histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA..
• Protein Sequence: MATESTAAAAIAAELVSADKIEDAPAPSTSADKMESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNVDEEAREELREQVYDAMGEKEAKKAEGKSLTKPETDKEQESEVEKGGREDMDISEPEEKLQETVEPTSKQLTESSEEAKEAAIPGLNEDEVASGKTEQESLCTEKGKSISGAYVQNKEFRETVEEGEEIISLEKKPKETSEDQPIRAAEKQGTLMKVVEIEAEIDPQVKSADVGGEEPKDQVATSESELGKAVLMELSGQDVEASPVVAAEAGAEVSEKPGQEITVIPNNGPVVGQSTVGDQTPSEPQTSAERLTETKDGSSVEEVKAELVPEQEEAMLPVEESEAAGDGVETKVAQKATEKAPEDKFKIAANEETPERDEQMKEGEETEGSEEEDRENDKAEETPNESVLEKKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYIQAVEEFQACLSLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVIEKRMAVLHEQMKEAEGSFTEYEKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPSGAGASVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVSSGKEVSENMEAEAENQAESQTAEGTVESAATIKSTAC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MATESTAAA ------CCCHHHHHH	21.78	-
17	Phosphorylation	AIAAELVSADKIEDA HHHHHHHCCHHHCCC	43.29	25338131
28	Phosphorylation	IEDAPAPSTSADKME HCCCCCCCCCHHHHH	36.78	25338131
29	Phosphorylation	EDAPAPSTSADKMES CCCCCCCCCHHHHHH	27.20	25338131
30	Phosphorylation	DAPAPSTSADKMESL CCCCCCCCHHHHHHC	38.52	27841257
33	Acetylation	APSTSADKMESLDVD CCCCCHHHHHHCCCC	44.26	-
52	Ubiquitination	KLLGLGQKHLVMGDI HHHCCCCCEEECCCH	36.79	-
76	Acetylation	AASLLGKKYGETANE HHHHHCHHHCCHHHH	57.60	22826441
93	Ubiquitination	EAFFFYGKSLLELAR HHHHHHHHHHHHHHH	26.93	-
96 (in isoform 2)	Phosphorylation	-	7.00	19854140
100 (in isoform 2)	Phosphorylation	-	45.64	26239621
111 (in isoform 2)	Phosphorylation	-	57.68	25521595
114 (in isoform 2)	Phosphorylation	-	31.93	25521595
123	Phosphorylation	EEEEGEKTEDESLVE CHHCCCCCCCHHHHC	44.35	25619855
127	Phosphorylation	GEKTEDESLVENNDN CCCCCCHHHHCCCCC	50.46	25619855
154	Acetylation	VYDAMGEKEAKKAEG HHHHHCHHHHHHHCC	58.19	23864654
157	Acetylation	AMGEKEAKKAEGKSL HHCHHHHHHHCCCCC	54.32	23864654
165	Phosphorylation	KAEGKSLTKPETDKE HHCCCCCCCCCCCHH	52.20	27180971
169	Phosphorylation	KSLTKPETDKEQESE CCCCCCCCCHHHHHH	61.98	27180971
175	Phosphorylation	ETDKEQESEVEKGGR CCCHHHHHHHHHCCC	46.79	25619855
188	Phosphorylation	GREDMDISEPEEKLQ CCCCCCCCCCHHHHH	42.72	26824392
203	Ubiquitination	ETVEPTSKQLTESSE HHCCCCCHHHHCCHH	52.37	-
206	Phosphorylation	EPTSKQLTESSEEAK CCCCHHHHCCHHHHH	31.18	25293948
208	Phosphorylation	TSKQLTESSEEAKEA CCHHHHCCHHHHHHH	37.50	22006019
209	Phosphorylation	SKQLTESSEEAKEAA CHHHHCCHHHHHHHH	32.97	25293948
227	Phosphorylation	LNEDEVASGKTEQES CCHHHHHCCCCCHHH	46.10	25293948
230	Phosphorylation	DEVASGKTEQESLCT HHHHCCCCCHHHHHC	46.56	25293948
234	Phosphorylation	SGKTEQESLCTEKGK CCCCCHHHHHCCCCC	28.56	25293948
237	Phosphorylation	TEQESLCTEKGKSIS CCHHHHHCCCCCCCC	46.13	25293948
239	Acetylation	QESLCTEKGKSISGA HHHHHCCCCCCCCCC	54.18	23806337
241	Acetylation	SLCTEKGKSISGAYV HHHCCCCCCCCCCEE	56.54	23806337
242	Phosphorylation	LCTEKGKSISGAYVQ HHCCCCCCCCCCEEC	30.56	29895711
244	Phosphorylation	TEKGKSISGAYVQNK CCCCCCCCCCEECCH	25.18	22006019
251	Ubiquitination	SGAYVQNKEFRETVE CCCEECCHHHHHHHH	40.93	-
251	Acetylation	SGAYVQNKEFRETVE CCCEECCHHHHHHHH	40.93	23806337
256	Phosphorylation	QNKEFRETVEEGEEI CCHHHHHHHHCCCCE	29.03	25338131
284	Acetylation	QPIRAAEKQGTLMKV CCCCHHHHCCCEEEE	49.67	23806337
303	Ubiquitination	AEIDPQVKSADVGGE EECCCCCCCCCCCCC	34.38	-
304	Phosphorylation	EIDPQVKSADVGGEE ECCCCCCCCCCCCCC	30.74	25293948
318	Phosphorylation	EPKDQVATSESELGK CCCHHCCCCHHHHHH	33.88	25293948
319	Phosphorylation	PKDQVATSESELGKA CCHHCCCCHHHHHHH	29.41	25293948
321	Phosphorylation	DQVATSESELGKAVL HHCCCCHHHHHHHHH	37.58	25293948
377	Phosphorylation	QSTVGDQTPSEPQTS CCCCCCCCCCCCCCC	33.26	-
384	Phosphorylation	TPSEPQTSAERLTET CCCCCCCCHHHHCCC	23.92	-
389	Phosphorylation	QTSAERLTETKDGSS CCCHHHHCCCCCCCC	48.07	21183079
391	Phosphorylation	SAERLTETKDGSSVE CHHHHCCCCCCCCHH	29.24	21183079
395	Phosphorylation	LTETKDGSSVEEVKA HCCCCCCCCHHHHHH	41.15	22006019
396	Phosphorylation	TETKDGSSVEEVKAE CCCCCCCCHHHHHHH	38.79	28973931
418	Phosphorylation	AMLPVEESEAAGDGV HCCCHHHHHHCCCCC	21.01	22817900
450	Phosphorylation	KIAANEETPERDEQM CCCCCCCCCCHHHHH	25.03	-
463	Phosphorylation	QMKEGEETEGSEEED HHHCCCCCCCCHHHH	41.15	27087446
466	Phosphorylation	EGEETEGSEEEDREN CCCCCCCCHHHHHCC	35.29	27087446
479	Phosphorylation	ENDKAEETPNESVLE CCCCCCCCCCHHHHH	23.95	21149613
483	Phosphorylation	AEETPNESVLEKKSL CCCCCCHHHHHHHHC	38.35	21149613
487	Ubiquitination	PNESVLEKKSLQENE CCHHHHHHHHCCCCC	43.43	-
488	Ubiquitination	NESVLEKKSLQENEE CHHHHHHHHCCCCCH	47.20	-
489	Phosphorylation	ESVLEKKSLQENEEE HHHHHHHHCCCCCHH	46.48	27180971
521	Ubiquitination	IFKRQETKEAQLYAA HHHCCCHHHHHHHHH	50.75	-
602	Acetylation	KSIDVIEKRMAVLHE HHHHHHHHHHHHHHH	36.03	22826441
612	Acetylation	AVLHEQMKEAEGSFT HHHHHHHHHHCCCCC	54.16	22826441
617	Phosphorylation	QMKEAEGSFTEYEKE HHHHHCCCCCHHHHH	22.09	26824392
619	Phosphorylation	KEAEGSFTEYEKEIE HHHCCCCCHHHHHHH	39.08	25619855
621	Phosphorylation	AEGSFTEYEKEIEEL HCCCCCHHHHHHHHH	29.28	25619855
623	Ubiquitination	GSFTEYEKEIEELKE CCCCHHHHHHHHHHH	64.19	-
629	Ubiquitination	EKEIEELKELLPEIR HHHHHHHHHHHHHHH	49.98	-
648	Phosphorylation	DAKESQRSGNVAELA HHHHHHHCCCHHHHH	27.38	-
664	Phosphorylation	KATLVESSTSGFTPS HHHHEECCCCCCCCC	17.06	26643407
665	Phosphorylation	ATLVESSTSGFTPSG HHHEECCCCCCCCCC	41.99	26643407
666	Phosphorylation	TLVESSTSGFTPSGA HHEECCCCCCCCCCC	33.58	26643407
669	Phosphorylation	ESSTSGFTPSGAGAS ECCCCCCCCCCCCCC	21.77	26643407
671	Phosphorylation	STSGFTPSGAGASVS CCCCCCCCCCCCCCE	37.91	26643407
684	Acetylation	VSMIASRKPTDGASS CEEEEECCCCCCCCC	50.18	23806337
684	Ubiquitination	VSMIASRKPTDGASS CEEEEECCCCCCCCC	50.18	-
686	Phosphorylation	MIASRKPTDGASSSN EEEECCCCCCCCCCC	50.53	25266776
690	Phosphorylation	RKPTDGASSSNCVTD CCCCCCCCCCCHHHH	39.57	25777480
691	Phosphorylation	KPTDGASSSNCVTDI CCCCCCCCCCHHHHH	25.65	25777480
692	Phosphorylation	PTDGASSSNCVTDIS CCCCCCCCCHHHHHH	31.58	25777480
694	Glutathionylation	DGASSSNCVTDISHL CCCCCCCHHHHHHHH	3.63	24333276
696	Phosphorylation	ASSSNCVTDISHLVR CCCCCHHHHHHHHHH	29.80	25777480
699	Phosphorylation	SNCVTDISHLVRKKR CCHHHHHHHHHHHCC	17.36	25777480
707	Ubiquitination	HLVRKKRKPEEESPR HHHHHCCCCCCCCCC	66.71	-
712	Phosphorylation	KRKPEEESPRKDDAK CCCCCCCCCCHHHHH	33.03	26824392
720	Acetylation	PRKDDAKKAKQEPEV CCHHHHHHHHHCCCC	63.64	6569377
731	Phosphorylation	EPEVNGGSGDAVSSG CCCCCCCCCCCHHCC	34.59	28285833
736	Phosphorylation	GGSGDAVSSGKEVSE CCCCCCHHCCHHHHH	34.78	28285833
737	Phosphorylation	GSGDAVSSGKEVSEN CCCCCHHCCHHHHHH	47.49	-
739	Acetylation	GDAVSSGKEVSENME CCCHHCCHHHHHHHH	57.82	8275565
742	Phosphorylation	VSSGKEVSENMEAEA HHCCHHHHHHHHHHH	25.09	-
755	Phosphorylation	EAENQAESQTAEGTV HHHHHHHHHCCCCCC	35.53	30635358
757	Phosphorylation	ENQAESQTAEGTVES HHHHHHHCCCCCCCH	35.64	30635358
761	Phosphorylation	ESQTAEGTVESAATI HHHCCCCCCCHHHHH	16.77	30635358

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NASP_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NASP_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NASP_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HSP72_MOUSE	Hspa2	physical	19553603
CDK1_MOUSE	Cdk1	physical	19553603

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.