dbPTM

MYPT1_CHICK - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MYPT1_CHICK
UniProt AC	Q90623
Protein Name	Protein phosphatase 1 regulatory subunit 12A
Gene Name	PPP1R12A
Organism	Gallus gallus (Chicken).
Sequence Length	1004
Subcellular Localization	Cytoplasm. Along actomyosin filaments and stress fibers..
Protein Description	Regulates myosin phosphatase activity..
Protein Sequence	MKMADAKQKRNEQLKRWIGSETDLEPPVVKRKKTKVKFDDGAVFLAACSSGDTEEVLRLLERGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEYLISQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGGTALHVAAAKGYTEVLKLLIQARYDVNIKDYDGWTPLHAAAHWGKEEACRILVENLCDMEAVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKREKKSPLIESTANLDNNQTQKTFKNKETLIMEQEKNASSIESLEHEKADEEEEGKKDESSCSSEEEEDDDSESEAETDKAKTLANANTTSTQSASMTAPSVAGGQGTPTSPLKKFPTSTTKVSPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDSAGVIRSASSPRLSSSLDNKEKEKDGKGTRLAYVAPTIPRRLASTSDIDEKENRDSSASSIRSGSSYARRKWEEDVKKNSLNEGPTSLNTSYQRSGSFGRRQDDLVSSNVPSTASTVTSSAGLQKTLPASANTTTKSTTGSTSAGVQSSTSNRLWAEDSTEKEKDSVPTAVTVPVAPSVVNAAATTTAMTTATSGTVSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETKDDDYRQRYSRTVEEPYHRYRPTSTSTSTSSTSSLSTSTSSLSSSSQLNRPNSLIGITSAYSRSGTKESEREGGKKEEEKEEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQEHQSDSEEGTNKKETQSDSLSRYDTGSLSVSSGDRYDSAQGRSGSQSYLEDRKPYCSRLEKEDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKTTQRQERFADRSLLEMEKRVSGKSQYLLGGKKSSRKKDI

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
299	Phosphorylation	SEKREKKSPLIESTA HHHHHCCCCCCCCCC	35.69	21736374
304	Phosphorylation	KKSPLIESTANLDNN CCCCCCCCCCCCCCC	26.31	23106611
305	Phosphorylation	KSPLIESTANLDNNQ CCCCCCCCCCCCCCC	13.53	23106611
438	Phosphorylation	WRLGLRKTGSYGALA HHHHCCCCCCHHHHE	25.56	23106611
440	Phosphorylation	LGLRKTGSYGALAEI HHCCCCCCHHHHEEH	26.14	21736374
495	Phosphorylation	RLAYVAPTIPRRLAS EEEEECCCCCCHHHC	33.60	12030846
667	Phosphorylation	EVRERRRSYLTPVRD HHHHHHHHHCCCCCC	23.76	-
694	Phosphorylation	QARQSRRSTQGVTLT HHHHHHHHCCCCCHH	24.79	-
695	Phosphorylation	ARQSRRSTQGVTLTD HHHHHHHCCCCCHHH	27.77	16297917
709	Phosphorylation	DLQEAEKTIGRSRST HHHHHHHHHCCCHHH	21.85	12030846
716	Phosphorylation	TIGRSRSTRTREQEN HHCCCHHHHHHHHHH	34.64	11976330
849	Phosphorylation	RPREKRRSTGVSFWT CCHHHHHHCCCCCCC	33.28	10601309
850	Phosphorylation	PREKRRSTGVSFWTQ CHHHHHHCCCCCCCC	39.53	7982954

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
495	T	Phosphorylation	Kinase	ILK	Q13418	PSP
654	T	Phosphorylation	Kinase	DMPK	-	GPS
667	S	Phosphorylation	Kinase	PRKG1	Q13976	GPS
694	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
694	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
694	S	Phosphorylation	Kinase	PRKG1	Q13976	GPS
695	T	Phosphorylation	Kinase	DAPK3	O43293	PSP
695	T	Phosphorylation	Kinase	ROCK2	-	Uniprot
695	T	Phosphorylation	Kinase	ILK	Q13418	PSP
695	T	Phosphorylation	Kinase	ROCK-SUBFAMILY	-	GPS
695	T	Phosphorylation	Kinase	PRKACA	P17612	GPS
695	T	Phosphorylation	Kinase	ROCK1	Q13464	PSP
695	T	Phosphorylation	Kinase	ROCK2	O75116	PSP
709	T	Phosphorylation	Kinase	ILK	Q13418	PSP
716	T	Phosphorylation	Kinase	DAPK3	O43293	PSP
849	S	Phosphorylation	Kinase	DAPK3	O43293	PSP
849	S	Phosphorylation	Kinase	ROCK1	Q13464	PSP
849	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
850	T	Phosphorylation	Kinase	ROCK1	Q13464	PSP
850	T	Phosphorylation	Kinase	PRKACA	P17612	GPS
850	T	Phosphorylation	Kinase	ROCK-SUBFAMILY	-	GPS
850	T	Phosphorylation	Kinase	ROCK2	F1NK88	GPS
850	T	Phosphorylation	Kinase	ROCK2	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of MYPT1_CHICK !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MYPT1_CHICK !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of MYPT1_CHICK !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MYPT1_CHICK

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Inhibitory phosphorylation site for Rho-associated kinase on smoothmuscle myosin phosphatase."; Feng J., Ito M., Ichikawa K., Isaka N., Nishikawa M., Hartshorne D.J.,Nakano T.; J. Biol. Chem. 274:37385-37390(1999). Cited for: PHOSPHORYLATION AT THR-695 AND THR-850, AND MUTAGENESIS OF THR-695 ANDTHR-850.	10601309 [Abstract]
"Phosphorylation of the regulatory subunit of smooth muscle proteinphosphatase 1M at Thr850 induces its dissociation from myosin."; Velasco G., Armstrong C., Morrice N., Frame S., Cohen P.; FEBS Lett. 527:101-104(2002). Cited for: PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY, AND PHOSPHORYLATION ATTHR-695 AND THR-850.	12220642 [Abstract]