dbPTM

MYH9_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MYH9_RAT
UniProt AC	Q62812
Protein Name	Myosin-9
Gene Name	Myh9
Organism	Rattus norvegicus (Rat).
Sequence Length	1961
Subcellular Localization	Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Colocalizes with actin filaments at lamellipodia margins and at the leading edge of migrating cells (By similarity). In retinal pigment epithelial cells, predominantly localized to stress fiber-like
Protein Description	During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (By similarity). Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping..
Protein Sequence	MAQQAADKYLYVDKNFINNPLAQADCGAKKLVWVPSTKNGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVDMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYLLSGAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIPEDEQMGLLRVISGVLQLGNIVFKKERNTDQASMPDNTAAQKVSHLLGINVTDFTRGILTPRIKVGRDYVQKAQTKEQADFAIEALAKATYERMFRWLVLRINKALDKTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIEKPAGPPGILALLDEECWFPKATDKSFVEKVVQEQGTHPKFQKPKQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSETALPGAFKTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVCCIIPNHEKKAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRVVFQEFRQRYEILTPNSIPKGFMDGKQACVLMIKALELDSNLYRIGQSKVFFRSGVLAHLEEERDLKITDVIIGFQACCRGYLARKAFAKRQQQLTAMKVLQRNCAAYLRLRNWQWWRLFTKVKPLLNSIRHEDELLAKEAELTKVREKHLAAENRLTEMETMQSQLMAEKLQLQEQLQAKTELCAEAEELRARLTAKKQELEEICHDLEARVEEEEERCQYLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEDQIIMEDQNCKLAKEKKLLEDRVAEFTTDLMEEEEKSKSLAKLKNKHEAMITDLEERLRREEKQRQELEKTRRKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQAALARVEEEAAQKNMALKKIRELETQISELQEDLESERACRNKAEKQKRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVSILKKTLEDEAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKATLEKAKQTLENERGELANEVKALLQGKGDSEHKRKKVEAQLQELQVKFSEGERVRTELADKVSKLQVELDSVTGLLNQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTKLKQMEDEKNSFREQLEEEEEEAKRNLEKQIATLHAQVTDMKKKMEDGVGCLETAEEAKRRLQKDLEGLSQRLEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRQSVSNLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLARALEEAMEQKAELERLNKQFRTEMEDLMSSKDDVGKSVHELEKSNRALEQQVEEMKTQLEELEDELQATEDAKLRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDERKQRSIAMAARKKLEMDLKDLEAHIDTANKNREEAIKQLRKLQAQMKDCMRDVDDTRASREEILAQAKENEKKLKSMEAEMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEALIALLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKAKLQEMESAVKSKYKASIAALEAKIAQLEEQLDNETKERQAASKQVRRAEKKLKDVLLQVEDERRNAEQFKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDMPFVVTRRIVRKGTGDCSDEEVDGKADGADAKATE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAQQAADKY ------CCHHHHHHH	22.84	-
8	Acetylation	MAQQAADKYLYVDKN CCHHHHHHHEEECCC	32.21	22902405
9	Phosphorylation	AQQAADKYLYVDKNF CHHHHHHHEEECCCC	12.10	23991683
11	Phosphorylation	QAADKYLYVDKNFIN HHHHHHEEECCCCCC	11.96	23991683
14	Acetylation	DKYLYVDKNFINNPL HHHEEECCCCCCCHH	43.65	55699609
36	Phosphorylation	KKLVWVPSTKNGFEP CEEEEECCCCCCCCC	42.25	23984901
37	Phosphorylation	KLVWVPSTKNGFEPA EEEEECCCCCCCCCC	23.58	23984901
74	Acetylation	VNKDDIQKMNPPKFS CCHHHHHHCCCCCCC	40.76	22902405
79	Acetylation	IQKMNPPKFSKVEDM HHHCCCCCCCCHHHH	64.62	22902405
102	Acetylation	ASVLHNLKERYYSGL HHHHHHHHHHHCCCE	45.52	22902405
139	Acetylation	EEIVDMYKGKKRHEM HHHHHHHCCCCCCCC	58.58	22902405
299	Acetylation	LLLEPYNKYRFLSNG EECCCCCCCEECCCC	31.77	22902405
355	Acetylation	QLGNIVFKKERNTDQ HHCCEEEECCCCCCC	42.71	22902405
360	Phosphorylation	VFKKERNTDQASMPD EEECCCCCCCCCCCC	35.39	28432305
364	Phosphorylation	ERNTDQASMPDNTAA CCCCCCCCCCCCHHH	25.80	28432305
369	Phosphorylation	QASMPDNTAAQKVSH CCCCCCCHHHHHHHH	30.65	25575281
407	Acetylation	YVQKAQTKEQADFAI HHHHHCCHHHHHHHH	35.43	-
435	Acetylation	WLVLRINKALDKTKR HHHHHHHHHHHHHHH	48.21	22902405
540	Acetylation	WFPKATDKSFVEKVV CCCCCCCHHHHHHHH	41.25	31095973
545	Acetylation	TDKSFVEKVVQEQGT CCHHHHHHHHHHCCC	42.26	22902405
555	Acetylation	QEQGTHPKFQKPKQL HHCCCCCCCCCCHHH	53.47	22902405
580	Acetylation	YAGKVDYKADEWLMK CCCCCCCCHHHHHHH	44.92	22902405
613	Acetylation	KFVSELWKDVDRIIG HHHHHHHHCHHHHCC	61.57	22902405
628	Phosphorylation	LDQVAGMSETALPGA HHHHCCCCCCCCCCC	30.77	-
638	Phosphorylation	ALPGAFKTRKGMFRT CCCCCHHHCCCCCHH	31.03	16641100
651	Acetylation	RTVGQLYKEQLAKLM HHHHHHHHHHHHHHH	47.80	22902405
656	Acetylation	LYKEQLAKLMATLRN HHHHHHHHHHHHHHH	47.60	22902405
679	Acetylation	IIPNHEKKAGKLDPH ECCCCHHCCCCCCHH	60.43	22902405
682	Acetylation	NHEKKAGKLDPHLVL CCHHCCCCCCHHHHH	55.35	22902405
721	Phosphorylation	FQEFRQRYEILTPNS HHHHHHHHCCCCCCC	10.05	28432305
725	Phosphorylation	RQRYEILTPNSIPKG HHHHCCCCCCCCCCC	26.53	28432305
728	Phosphorylation	YEILTPNSIPKGFMD HCCCCCCCCCCCCCC	41.28	28432305
751	Phosphorylation	IKALELDSNLYRIGQ HHHEECCCCCEEECC	41.16	22673903
754	Phosphorylation	LELDSNLYRIGQSKV EECCCCCEEECCCCH	12.39	22673903
833	Acetylation	QWWRLFTKVKPLLNS HHHHHHHHHHHHHHH	40.71	22902405
850	Succinylation	HEDELLAKEAELTKV CHHHHHHHHHHHHHH	58.38	-
850	Succinylation	HEDELLAKEAELTKV CHHHHHHHHHHHHHH	58.38	-
860	Acetylation	ELTKVREKHLAAENR HHHHHHHHHHHHHHH	33.10	-
910	Succinylation	RARLTAKKQELEEIC HHHHHHHHHHHHHHH	46.68	26843850
933	Phosphorylation	EEEERCQYLQAEKKK HHHHHHHHHHHHHHH	12.38	27097102
961	Acetylation	EEESARQKLQLEKVT HHHHHHHHHHHHHHH	32.42	72602209
966	Acetylation	RQKLQLEKVTTEAKL HHHHHHHHHHHHHHH	54.17	-
972	Acetylation	EKVTTEAKLKKLEED HHHHHHHHHHHHHHC	55.39	22902405
975	Acetylation	TTEAKLKKLEEDQII HHHHHHHHHHHCCCC	72.12	-
975	Succinylation	TTEAKLKKLEEDQII HHHHHHHHHHHCCCC	72.12	26843850
1020	Acetylation	EKSKSLAKLKNKHEA HHHHHHHHHCHHHHH	66.35	22902405
1022	Acetylation	SKSLAKLKNKHEAMI HHHHHHHCHHHHHHH	64.34	22902405
1024	Acetylation	SLAKLKNKHEAMITD HHHHHCHHHHHHHHH	41.03	22902405
1030	Phosphorylation	NKHEAMITDLEERLR HHHHHHHHHHHHHHH	22.80	22673903
1048	Acetylation	KQRQELEKTRRKLEG HHHHHHHHHHHHHHC	61.01	22902405
1049	Phosphorylation	QRQELEKTRRKLEGD HHHHHHHHHHHHHCC	26.74	30181290
1080	Acetylation	ELKMQLAKKEEELQA HHHHHHHHHHHHHHH	70.12	22902405
1081	Acetylation	LKMQLAKKEEELQAA HHHHHHHHHHHHHHH	65.83	22902405
1081	Succinylation	LKMQLAKKEEELQAA HHHHHHHHHHHHHHH	65.83	26843850
1111	Phosphorylation	KKIRELETQISELQE HHHHHHHHHHHHHHH	43.77	22668510
1114	Phosphorylation	RELETQISELQEDLE HHHHHHHHHHHHHHH	23.57	21630457
1146	Phosphorylation	EELEALKTELEDTLD HHHHHHHHHHHHHHC	46.80	30181290
1151	Phosphorylation	LKTELEDTLDSTAAQ HHHHHHHHHCHHHHH	24.08	30181290
1154	Phosphorylation	ELEDTLDSTAAQQEL HHHHHHCHHHHHHHH	24.06	30181290
1155	Phosphorylation	LEDTLDSTAAQQELR HHHHHCHHHHHHHHH	26.19	30181290
1173	Acetylation	EQEVSILKKTLEDEA HHHHHHHHHHHHHHH	41.88	22902405
1181	Acetylation	KTLEDEAKTHEAQIQ HHHHHHHHHHHHHHH	48.65	22902405
1209	Acetylation	AEQLEQTKRVKATLE HHHHHHHHHHHHHHH	55.69	22902405
1212	Acetylation	LEQTKRVKATLEKAK HHHHHHHHHHHHHHH	39.42	22902405
1234	Acetylation	GELANEVKALLQGKG HHHHHHHHHHHCCCC	27.43	22902405
1248	Acetylation	GDSEHKRKKVEAQLQ CCHHHHHHHHHHHHH	67.01	22902405
1249	Acetylation	DSEHKRKKVEAQLQE CHHHHHHHHHHHHHH	49.71	22902405
1277	Acetylation	ELADKVSKLQVELDS HHHHHHHHHHHHHHH	46.02	22902405
1301	Acetylation	SKSSKLTKDFSALES CCCCHHHHHHHHHHH	68.18	22902405
1308	Phosphorylation	KDFSALESQLQDTQE HHHHHHHHHHHHHHH	36.72	27097102
1313	Phosphorylation	LESQLQDTQELLQEE HHHHHHHHHHHHHHH	15.81	27097102
1330	Acetylation	QKLSLSTKLKQMEDE HHCCHHHHHHHHHHH	50.76	22902405
1332	Acetylation	LSLSTKLKQMEDEKN CCHHHHHHHHHHHHH	49.51	22902405
1338	Acetylation	LKQMEDEKNSFREQL HHHHHHHHHHHHHHH	70.93	22902405
1358	Acetylation	EAKRNLEKQIATLHA HHHHHHHHHHHHHHH	50.61	22902405
1371	Acetylation	HAQVTDMKKKMEDGV HHHHHHHHHHHHCCC	52.64	22902405
1393	Acetylation	EAKRRLQKDLEGLSQ HHHHHHHHHHHHHHH	69.72	22902405
1405	Acetylation	LSQRLEEKVAAYDKL HHHHHHHHHHHHHHH	28.00	22902405
1409	Phosphorylation	LEEKVAAYDKLEKTK HHHHHHHHHHHHHHH	12.13	-
1411	Acetylation	EKVAAYDKLEKTKTR HHHHHHHHHHHHHHH	45.96	22902405
1442	Acetylation	QSVSNLEKKQKKFDQ HHHHHHHHHHHHHHH	64.77	22902405
1445	Acetylation	SNLEKKQKKFDQLLA HHHHHHHHHHHHHHH	65.37	22902405
1446	Acetylation	NLEKKQKKFDQLLAE HHHHHHHHHHHHHHH	52.24	22902405
1446	Succinylation	NLEKKQKKFDQLLAE HHHHHHHHHHHHHHH	52.24	26843850
1455	Acetylation	DQLLAEEKTISAKYA HHHHHHHHHHHHHHH	42.54	22902405
1460	Acetylation	EEKTISAKYAEERDR HHHHHHHHHHHHHHH	37.90	22902405
1475	Acetylation	AEAEAREKETKALSL HHHHHHHHHHHHHHH	66.19	22902405
1478	Acetylation	EAREKETKALSLARA HHHHHHHHHHHHHHH	48.71	22902405
1493	Acetylation	LEEAMEQKAELERLN HHHHHHHHHHHHHHH	30.44	22902405
1501	Acetylation	AELERLNKQFRTEME HHHHHHHHHHHHHHH	56.24	22902405
1514	Acetylation	MEDLMSSKDDVGKSV HHHHHCCCCHHHHHH	51.12	22902405
1519	Acetylation	SSKDDVGKSVHELEK CCCCHHHHHHHHHHH	49.51	22902405
1556	Acetylation	LQATEDAKLRLEVNL HHCCHHHHHHHHHHH	46.60	-
1614	Acetylation	SIAMAARKKLEMDLK HHHHHHHHHHCCCHH	57.91	22902405
1615	Acetylation	IAMAARKKLEMDLKD HHHHHHHHHCCCHHH	44.09	22902405
1621	Acetylation	KKLEMDLKDLEAHID HHHCCCHHHHHHHHH	56.88	22902405
1629	Phosphorylation	DLEAHIDTANKNREE HHHHHHHHCCCCHHH	31.37	22673903
1639	Acetylation	KNREEAIKQLRKLQA CCHHHHHHHHHHHHH	50.78	22902405
1649	Acetylation	RKLQAQMKDCMRDVD HHHHHHHHHHHHCCC	35.38	72609079
1670	Acetylation	EEILAQAKENEKKLK HHHHHHHHHHHHHHH	49.33	22902405
1670	Succinylation	EEILAQAKENEKKLK HHHHHHHHHHHHHHH	49.33	-
1670	Succinylation	EEILAQAKENEKKLK HHHHHHHHHHHHHHH	49.33	-
1714	Phosphorylation	LADEIANSSGKGALA HHHHHHHCCCCCHHH	31.29	27097102
1715	Phosphorylation	ADEIANSSGKGALAL HHHHHHCCCCCHHHH	44.31	27097102
1725	Acetylation	GALALEEKRRLEALI CHHHHHHHHHHHHHH	33.18	72611687
1794	Acetylation	QNKELKAKLQEMESA HHHHHHHHHHHHHHH	49.46	22902405
1800	Phosphorylation	AKLQEMESAVKSKYK HHHHHHHHHHHHHHH	36.73	16641100
1803	Acetylation	QEMESAVKSKYKASI HHHHHHHHHHHHHHH	40.73	22902405
1816	Acetylation	SIAALEAKIAQLEEQ HHHHHHHHHHHHHHH	29.24	22902405
1829	Acetylation	EQLDNETKERQAASK HHHCHHHHHHHHHHH	44.73	22902405
1844	Acetylation	QVRRAEKKLKDVLLQ HHHHHHHHHHHHHHH	52.88	22902405
1846	Acetylation	RRAEKKLKDVLLQVE HHHHHHHHHHHHHHH	55.46	22902405
1846	Succinylation	RRAEKKLKDVLLQVE HHHHHHHHHHHHHHH	55.46	26843850
1863	Acetylation	RRNAEQFKDQADKAS HHCHHHHHHHHHHHH	48.93	22902405
1905	Phosphorylation	QRELEDATETADAMN HHHHHHHHHHHHHHH	46.30	25575281
1907	Phosphorylation	ELEDATETADAMNRE HHHHHHHHHHHHHHH	26.03	23984901
1916	Phosphorylation	DAMNREVSSLKNKLR HHHHHHHHHHHHHHH	25.79	23984901
1917	Phosphorylation	AMNREVSSLKNKLRR HHHHHHHHHHHHHHC	48.84	28689409
1924	Methylation	SLKNKLRRGDMPFVV HHHHHHHCCCCCEEE	56.77	-
1938	Succinylation	VTRRIVRKGTGDCSD EEEECEECCCCCCCH	50.35	26843850
1940	Phosphorylation	RRIVRKGTGDCSDEE EECEECCCCCCCHHH	32.65	23991683
1944	Phosphorylation	RKGTGDCSDEEVDGK ECCCCCCCHHHCCCC	53.44	23991683

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1917	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS
1940	T	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
1940	T	Phosphorylation	Kinase	CAMK2A	P11798	PSP
1940	T	Phosphorylation	Kinase	CAMK4	Q16566	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of MYH9_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MYH9_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of MYH9_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MYH9_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomics of vasopressin-sensitive renal cells:regulation of aquaporin-2 phosphorylation at two sites."; Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.; Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-638; SER-1800 ANDSER-1944, AND MASS SPECTROMETRY.	16641100 [Abstract]