MSH2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MSH2_RAT
• UniProt AC: P54275
• Protein Name: DNA mismatch repair protein Msh2
• Gene Name: Msh2
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 933
• Subcellular Localization: Nucleus .
• Protein Description: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis (By similarity)..
• Protein Sequence: MAVQPKETLQLEGAAEVGFVRFFEGMPEKPSTTVGLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKTLQTVVLSKMNFESFVKDLLLVRHYRVEVYKNKAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMATSIGIMGIKLSTVDGQRQVGVGDVDSTQRKLGLCEFPDNDQFSNLEALLIQIGPKECILPGGETAGDMGKLRQVIQRGGILITERKRIDFSTKDIYQDLNRLLKGRKGEQMNSAVLPEMENQVAVSSLSAVIKFLELLSDDSNFGQFELATFDFSQYMKLDMAAVRALNLFQGSVEDTTGSQSLAAFLNKCKTAQGQRLVSQWIKQPLMDKNRIEERLNLVEAFVEDSELRRALQEDLLRRFPDLNRLAKKFQRQAANLQDCYRLYQGVKQLPNVIQALEKYQGRHQALLLAVFVTPLTDLRSDFSKFQEKIETTLDMDQVENHEFLVKPSFDPNLSELREVMDGLEKKMQSTLISAARGLGLDPGKQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSELSSLNEEYTKNKGEYEEAQDAIVKEIVNISSGYVEPMQTVNDVLAHLDAVVSFAHVSNAAPVPYVRPVILEKGKGRIIVKASRHACVEVQHDVAFIPNDVHFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLETASILRSATKDSLIIIDELGRGTSTYDGFGLAWAISEYIATNIGAFCMFATHFHELTALASQIPTVNNLHVTALTTEETLTMLYQVKTGVCDQSFGIHVAELANFPRHVIECAKQKALELEEFQSIGTSQGHDETQPAAKRRCLEREQGEKIILEFLSKVKQVPFTDLSEESVSVKLKQLKAEVLAKNNSFVNEIISRVKAP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAVQPKETL ------CCCCCCCCE	14.51	-
66	Phosphorylation	KTQGVIKYMGPAGAK HHHCCHHHCCCCCCC	9.00	-
110	Ubiquitination	YKNKAGNKASKENDW EECCCCCCCCCCCCE	53.79	-
113	Ubiquitination	KAGNKASKENDWYLA CCCCCCCCCCCEEEE	66.45	-
567	Acetylation	NEEYTKNKGEYEEAQ CHHHHCCCCCHHHHH	55.46	-
897	Phosphorylation	KVKQVPFTDLSEESV CCCCCCCCCCCCCHH	29.93	22817900
921	Phosphorylation	EVLAKNNSFVNEIIS HHHHHCCHHHHHHHH	39.71	27097102

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MSH2_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MSH2_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MSH2_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of MSH2_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.