MRP_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MRP_RAT
• UniProt AC: Q9EPH2
• Protein Name: MARCKS-related protein
• Gene Name: Marcksl1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 199
• Subcellular Localization: Cytoplasm. Cell membrane ; Lipid-anchor . Associates with the membrane via the insertion of the N-terminal N-myristoyl chain and the partial insertion of the effector domain. Association of the effector domain with membranes may be regulated by Ca(2+
• Protein Description: Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems (By similarity)..
• Protein Sequence: MGSQSSKAPRGDVTAEEAAGASPAKANGQENGHVKSNGDLTPKGEGESPPVNGADEAAGATGDAIEPAPPSQEAEAKGEVAPKETPKKKKKFSFKKPFKLSGLSFKRNRKEGGGDSSASSPTEEEQEQGEISACSDEGTAQEGKAAATPESQEPQAKGAEASAVSKGGDAEEEAGPQAAEPSTPSGPESGPASASAENE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Myristoylation	------MGSQSSKAP ------CCCCCCCCC	34.10	-
14	Phosphorylation	KAPRGDVTAEEAAGA CCCCCCCCHHHHCCC	32.84	23298284
22	Phosphorylation	AEEAAGASPAKANGQ HHHHCCCCCCHHCCC	25.19	23712012
36	Phosphorylation	QENGHVKSNGDLTPK CCCCCCCCCCCCCCC	44.98	27097102
41	Phosphorylation	VKSNGDLTPKGEGES CCCCCCCCCCCCCCC	27.91	23298284
48	Phosphorylation	TPKGEGESPPVNGAD CCCCCCCCCCCCCCH	45.41	23984901
61	Phosphorylation	ADEAAGATGDAIEPA CHHHCCCCCCCCCCC	34.38	23984901
71	Phosphorylation	AIEPAPPSQEAEAKG CCCCCCCCHHHHHCC	39.32	27097102
85	Phosphorylation	GEVAPKETPKKKKKF CCCCCCCCCCCCCCC	46.68	23712012
91	Acetylation	ETPKKKKKFSFKKPF CCCCCCCCCCCCCCC	55.94	22902405
93	Phosphorylation	PKKKKKFSFKKPFKL CCCCCCCCCCCCCCC	45.06	23298284
95	Acetylation	KKKKFSFKKPFKLSG CCCCCCCCCCCCCCC	59.34	22902405
96	Acetylation	KKKFSFKKPFKLSGL CCCCCCCCCCCCCCC	54.39	22902405
99	Acetylation	FSFKKPFKLSGLSFK CCCCCCCCCCCCEEE	50.71	22902405
101	Phosphorylation	FKKPFKLSGLSFKRN CCCCCCCCCCEEECC	37.97	27097102
104	Phosphorylation	PFKLSGLSFKRNRKE CCCCCCCEEECCCCC	32.04	23984901
106	Acetylation	KLSGLSFKRNRKEGG CCCCCEEECCCCCCC	45.44	22902405
116	Phosphorylation	RKEGGGDSSASSPTE CCCCCCCCCCCCCCH	30.95	30240740
117	Phosphorylation	KEGGGDSSASSPTEE CCCCCCCCCCCCCHH	36.59	30240740
119	Phosphorylation	GGGDSSASSPTEEEQ CCCCCCCCCCCHHHH	38.51	30240740
120	Phosphorylation	GGDSSASSPTEEEQE CCCCCCCCCCHHHHH	35.24	30240740
122	Phosphorylation	DSSASSPTEEEQEQG CCCCCCCCHHHHHHC	58.96	30240740
132	Phosphorylation	EQEQGEISACSDEGT HHHHCCCEECCCCCC	20.94	28551015
135	Phosphorylation	QGEISACSDEGTAQE HCCCEECCCCCCHHC	38.11	30240740
139	Phosphorylation	SACSDEGTAQEGKAA EECCCCCCHHCCCCC	24.10	28432305
148	Phosphorylation	QEGKAAATPESQEPQ HCCCCCCCCCCCCCC	23.90	23712012
151	Phosphorylation	KAAATPESQEPQAKG CCCCCCCCCCCCCCC	40.84	23298284
162	Phosphorylation	QAKGAEASAVSKGGD CCCCCHHCHHHCCCC	22.25	30240740
165	Phosphorylation	GAEASAVSKGGDAEE CCHHCHHHCCCCHHH	25.87	-
182	Phosphorylation	GPQAAEPSTPSGPES CCCCCCCCCCCCCCC	44.44	27097102
183	Phosphorylation	PQAAEPSTPSGPESG CCCCCCCCCCCCCCC	31.99	27097102
185	Phosphorylation	AAEPSTPSGPESGPA CCCCCCCCCCCCCCC	68.05	27097102
189	Phosphorylation	STPSGPESGPASASA CCCCCCCCCCCCCCC	53.89	27097102
193	Phosphorylation	GPESGPASASAENE- CCCCCCCCCCCCCC-	26.37	27097102
195	Phosphorylation	ESGPASASAENE--- CCCCCCCCCCCC---	33.35	27097102

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
93	S	Phosphorylation	Kinase	PKC	-	Uniprot
101	S	Phosphorylation	Kinase	PKC	-	Uniprot
104	S	Phosphorylation	Kinase	PKC	-	Uniprot
120	S	Phosphorylation	Kinase	MAPK8	P49185	Uniprot
148	T	Phosphorylation	Kinase	MAPK8	P49185	Uniprot
183	T	Phosphorylation	Kinase	MAPK8	P49185	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
120	S	Phosphorylation	Phosphorylation at Ser-120 and Thr-183 is non-redundantly catalyzed by MAPK8 in vivo.	-
148	T	Phosphorylation	Phosphorylation at Thr-148 is preferentially catalyzed by MAPK8 in vivo, but this modification can also be catalyzed by other kinases in the absence of MAPK8.	-
183	T	Phosphorylation	Phosphorylation at Ser-120 and Thr-183 is non-redundantly catalyzed by MAPK8 in vivo.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MRP_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of MRP_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.