MPK8_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MPK8_ARATH
• UniProt AC: Q9LM33
• Protein Name: Mitogen-activated protein kinase 8
• Gene Name: MPK8
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 589
• Protein Description: MKK3-MPK8 and CAMs-MPK8 modules negatively regulates ROS accumulation through controlling expression of the RBOHD gene during wounding..
• Protein Sequence: MGGGGNLVDGVRRWLFQRPSSSSSSSSSNNNNNNHEQPIFNSSSFSSSSNPNHSANSGELIIEEDLDFSGLTLINVPKRNHLPMDPHKKGETEFFTEYGEANRYQIQEVVGKGSYGVVASAVDSHTGERVAIKKINDVFEHVSDATRILREIKLLRLLRHPDVVEIKHIMLPPSRREFRDIYVVFELMESDLHQVIKANDDLTPEHYQFFLYQLLRGLKYVHAANVFHRDLKPKNILANADCKLKICDFGLARVSFNDAPTAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSVGCIFAEMLLGKPLFPGKNVVHQLDLMTDFLGTPPPESISRIRNEKARRYLSSMRKKQPVPFSHKFPKADPLALRLLERLLAFDPKDRASAEDALADPYFSGLSNSEREPTTQPISKLEFDFERKKLVKDDVRELIYREILEYHPQMLEEYLRGGDQLSFMYPSGVDRFKRQFAHLEENQGKPGAAGGGRSTALHRHHASLPRERVPAPNGETAEESSDVERRAAAAVASTLESEEADNGGGYSARNLMKSASISGSKCIGVQSKTDKEDTIAEEEDNETVAELTDKVASLHNS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
266	Phosphorylation	APTAIFWTDYVATRW CCCEEEEEHHHHCCH	13.50	30407730
268	Phosphorylation	TAIFWTDYVATRWYR CEEEEEHHHHCCHHC	5.66	30291188
271	Phosphorylation	FWTDYVATRWYRAPE EEEHHHHCCHHCCHH	16.22	22074104
495	Phosphorylation	ALHRHHASLPRERVP HHHHCCCCCCHHCCC	34.25	23111157
508	Phosphorylation	VPAPNGETAEESSDV CCCCCCCCHHCCCHH	40.54	19880383
512	Phosphorylation	NGETAEESSDVERRA CCCCHHCCCHHHHHH	24.28	19880383
513	Phosphorylation	GETAEESSDVERRAA CCCHHCCCHHHHHHH	49.29	19880383
526	Phosphorylation	AAAAVASTLESEEAD HHHHHHHHHHHHHCC	25.47	22631563
529	Phosphorylation	AVASTLESEEADNGG HHHHHHHHHHCCCCC	43.80	22631563
538	Phosphorylation	EADNGGGYSARNLMK HCCCCCCCCHHHHHH	11.48	23776212
539	Phosphorylation	ADNGGGYSARNLMKS CCCCCCCCHHHHHHH	24.83	30291188
548	Phosphorylation	RNLMKSASISGSKCI HHHHHHCCCCCCEEE	25.12	23111157
550	Phosphorylation	LMKSASISGSKCIGV HHHHCCCCCCEEEEE	34.22	25561503

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MPK8_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
266	T	Phosphorylation	Dually phosphorylated on Thr-266 and Tyr-268, which activates the enzyme (By similarity).	21419340

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MPK8_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PUB14_ARATH	PUB14	physical	21798944

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.