MKNK2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MKNK2_MOUSE
• UniProt AC: Q8CDB0
• Protein Name: MAP kinase-interacting serine/threonine-protein kinase 2
• Gene Name: Mknk2
• Organism: Mus musculus (Mouse).
• Sequence Length: 459
• Subcellular Localization: Cytoplasm. Nucleus, PML body.
• Protein Description: Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Enhances the formation of EIF4F complex in pachytene spermatocytes, thus promoting mRNA translation during spermatogenesis. Displays a high basal kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As(2)O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal..
• Protein Sequence: MVQKRTAELQGFHRSFKGQNPFELAFSLDLAQHRDSDFSPQCEARPDMPSSQPIDIPDAKKRGRKKKRCRATDSFSGRFEDVYQLQEDVLGEGAHARVQTCVNLITNQEYAVKIIEKQLGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHRRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGHCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWSHISFAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPLVLQRNSCAKDLTSFAAEAIAMNRQLAQCEEDAGQDQPVVIRATSRCLQLSPPSQSKLAQRRQRASLSATPVVLVGDRA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MVQKRTAELQGFH --CCCHHHHHHCHHH	31.36	28973931
57 (in isoform 2)	Phosphorylation	-	31.58	23811945
61 (in isoform 2)	Phosphorylation	-	62.90	23811945
63 (in isoform 2)	Phosphorylation	-	55.42	23811945
72	Phosphorylation	KKKRCRATDSFSGRF CCCCCCCCCCCCCCH	16.90	26643407
74	Phosphorylation	KRCRATDSFSGRFED CCCCCCCCCCCCHHH	19.56	23684622
76	Phosphorylation	CRATDSFSGRFEDVY CCCCCCCCCCHHHHH	32.77	25266776
166	Phosphorylation	FEKMRGGSILSHIHR EEECCCCCHHHHHHH	24.15	-
169	Phosphorylation	MRGGSILSHIHRRRH CCCCCHHHHHHHHHC	20.80	-
220	Phosphorylation	CEHPNQVSPVKICDF ECCCCCCCCEEECCE	17.76	26824392
231	Phosphorylation	ICDFDLGSGIKLNGD ECCEECCCCCEECCC	44.64	-
244	Phosphorylation	GDCSPISTPELLTPC CCCCCCCCCHHHCCC	22.85	9155017
249	Phosphorylation	ISTPELLTPCGSAEY CCCCHHHCCCCCHHH	29.20	9155017
379	Phosphorylation	APENTLPTPLVLQRN CCCCCCCCCHHCCCC	32.16	9155017
387	Phosphorylation	PLVLQRNSCAKDLTS CHHCCCCCCHHHHHH	20.03	26060331
431	Phosphorylation	TSRCLQLSPPSQSKL ECHHHHCCCCCHHHH	23.08	25266776
434	Phosphorylation	CLQLSPPSQSKLAQR HHHCCCCCHHHHHHH	50.93	26745281
436	Phosphorylation	QLSPPSQSKLAQRRQ HCCCCCHHHHHHHHH	33.69	26745281
446	Phosphorylation	AQRRQRASLSATPVV HHHHHHHHHCCCCEE	25.66	26824392
448	Phosphorylation	RRQRASLSATPVVLV HHHHHHHCCCCEEEE	27.83	25266776
450	Phosphorylation	QRASLSATPVVLVGD HHHHHCCCCEEEECC	17.15	16141072

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MKNK2_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
244	T	Phosphorylation	Dual phosphorylation of Thr-244 and Thr-249 activates the kinase.	11154262
249	T	Phosphorylation	Dual phosphorylation of Thr-244 and Thr-249 activates the kinase.	11154262
379	T	Phosphorylation	Phosphorylation of Thr-379 activates the kinase.	11154262

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MKNK2_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of MKNK2_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"The mitogen-activated protein kinase signal-integrating kinase Mnk2is a eukaryotic initiation factor 4E kinase with high levels of basalactivity in mammalian cells.";
Scheper G.C., Morrice N.A., Kleijn M., Proud C.G.;
Mol. Cell. Biol. 21:743-754(2001).
Cited for: FUNCTION AS EIF4E KINASE, PHOSPHORYLATION AT SER-74; SER-431; SER-434;SER-446 AND THR-450, PHOSPHORYLATION BY MAPK1/ERK2; MAPK11 AND MAPK14,AND INTERACTION WITH EIF4G PROTEINS.
PubMed: 11154262