MET16_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MET16_MOUSE
• UniProt AC: Q9CQG2
• Protein Name: U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase {ECO:0000250|UniProtKB:Q86W50}
• Gene Name: Mettl16 {ECO:0000312|MGI:MGI:1914743}
• Organism: Mus musculus (Mouse).
• Sequence Length: 553
• Subcellular Localization: Nucleus .
• Protein Description: RNA N6-methyltransferase that methylates adenosine residues of a subset of RNAs and plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. [PubMed: 29262316 Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (By similarity In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (By similarity In presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression]
• Protein Sequence: MALSKSMHARNRYKDKPPDFAYLASKYPDFKQHIQINLNGRVSLNFKDPEAVRALTCTLLREDFGLSIDIPLERLIPTVPLRLNYIHWVEDLIGHQDSDKTTLRRGIDIGTGASCIYPLLGATLNGWYFLATEVDDMCFNYAKKNVEQNNLSDLIKVVKVPQKTLLMDALKEESEIVYDFCMCNPPFFANQLEAKGVNSRNSRRPPPSSVNTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSCMLGKKCSLAPLKEELRIQGVPKVTFTEFCQGRTMRWALAWSFYDDVTVPSPPSKRRKLEKPRKPITFVVLESVMKELSLKASSLGSETAEGIVVVTTWIEKILTDLKVQHKRIPCGREEVSLFLTAIENSWIHLRRKRRERVRQLREVPRAPEDVILALEERKSTPKELSSGQDVAHSPQESALCGLDVPGGEAAADGGHCLSQKLLCQEETPEATEDERDEERGGMEAMESCKGSSNGAQDGEASEKGDRLDGAAGRYLFKCLVNIKKEAGDAVVEMHWVEGQNRDLMNQLCTYVRNQILRLVAS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
22	Phosphorylation	DKPPDFAYLASKYPD CCCCCHHHHHHHCCC	11.64	-
27	Phosphorylation	FAYLASKYPDFKQHI HHHHHHHCCCHHHHE	12.92	-
67	Phosphorylation	LREDFGLSIDIPLER HHHHHCCCCEEEHHH	20.70	21743459
294	Phosphorylation	WSFYDDVTVPSPPSK HHCCCCCCCCCCHHH	33.42	26643407
297	Phosphorylation	YDDVTVPSPPSKRRK CCCCCCCCCHHHCCC	45.14	26643407
300	Phosphorylation	VTVPSPPSKRRKLEK CCCCCCHHHCCCCCC	42.02	26643407
325	Phosphorylation	ESVMKELSLKASSLG HHHHHHHCHHHHHCC	28.99	28059163
329	Phosphorylation	KELSLKASSLGSETA HHHCHHHHHCCCCCC	25.22	-
411	Phosphorylation	LALEERKSTPKELSS HHHHHCCCCCCCCCC	57.05	26160508
412	Phosphorylation	ALEERKSTPKELSSG HHHHCCCCCCCCCCC	41.22	26160508
417	Phosphorylation	KSTPKELSSGQDVAH CCCCCCCCCCCCCCC	33.25	27742792
418	Phosphorylation	STPKELSSGQDVAHS CCCCCCCCCCCCCCC	53.77	27742792
425	Phosphorylation	SGQDVAHSPQESALC CCCCCCCCCCHHCCC	20.67	25521595
429	Phosphorylation	VAHSPQESALCGLDV CCCCCCHHCCCCCCC	22.69	27742792
450	Phosphorylation	ADGGHCLSQKLLCQE CCCCCCCCHHCEECC	30.57	25619855
459	Phosphorylation	KLLCQEETPEATEDE HCEECCCCCCCCCCH	26.19	25521595
463	Phosphorylation	QEETPEATEDERDEE CCCCCCCCCCHHHHH	42.16	27087446
493	Phosphorylation	GAQDGEASEKGDRLD CCCCCCCCCCCCCCC	35.22	23684622

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MET16_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MET16_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MET16_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of MET16_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage.";
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
Science 316:1160-1166(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450, AND MASSSPECTROMETRY.
PubMed: 17525332