MEP50_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MEP50_MOUSE
• UniProt AC: Q99J09
• Protein Name: Methylosome protein 50
• Gene Name: Wdr77
• Organism: Mus musculus (Mouse).
• Sequence Length: 342
• Subcellular Localization: Nucleus . Cytoplasm .
• Protein Description: Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. [PubMed: 19584108]
• Protein Sequence: MRKDTPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGSLLLGVSSLSGRCWVGSLWFFKDPSAAPNEGFCSAGVQTEAGVADLTWVGDKGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVTVLSSGTQAVSGSKDCCIKIWDLAQQVSLNSYRAHAGQVTCVAASPHKDSVFLSCSEDSRILLWDTRCPKPASQMACNASGYLPTALAWHPQQSEVFVFGDENGSVSLVDTKNASCTLSSAVHSQGVTRLVFSPHSVPLLTSLSEDCSLAVLDSSLSEVFRSRAHRDFVRDATWSPLNHSLLTTVGWDHQVIHHVVPLEPLPNPGPDSVVE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MRKDTPPPLVPP ---CCCCCCCCCCCC	40.50	26824392
26	Glutathionylation	LPPNAPACMERQLEA CCCCCCHHHHHHHHH	2.57	24333276
159	Phosphorylation	WDLAQQVSLNSYRAH HHHHHHHCCCHHHHC	19.93	25168779
162	Phosphorylation	AQQVSLNSYRAHAGQ HHHHCCCHHHHCCCE	22.68	25168779
163	Phosphorylation	QQVSLNSYRAHAGQV HHHCCCHHHHCCCEE	15.67	25168779
171	Phosphorylation	RAHAGQVTCVAASPH HHCCCEEEEEECCCC	8.06	25168779
172	S-nitrosocysteine	AHAGQVTCVAASPHK HCCCEEEEEECCCCC	1.74	-
172	S-nitrosylation	AHAGQVTCVAASPHK HCCCEEEEEECCCCC	1.74	21278135
176	Phosphorylation	QVTCVAASPHKDSVF EEEEEECCCCCCCEE	20.14	25168779
247	Glutathionylation	VDTKNASCTLSSAVH EECCCCCEEECHHHH	4.05	24333276

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MEP50_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MEP50_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MEP50_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of MEP50_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5, AND MASSSPECTROMETRY.
PubMed: 19367708