MATK_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MATK_HUMAN
• UniProt AC: P42679
• Protein Name: Megakaryocyte-associated tyrosine-protein kinase
• Gene Name: MATK
• Organism: Homo sapiens (Human).
• Sequence Length: 507
• Subcellular Localization: Cytoplasm . Membrane . In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation.
• Protein Description: Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation..
• Protein Sequence: MAGRGSLVSWRAFHGCDSAEELPRVSPRFLRAWHPPPVSARMPTRRWAPGTQCITKCEHTRPKPGELAFRKGDVVTILEACENKSWYRVKHHTSGQEGLLAAGALREREALSADPKLSLMPWFHGKISGQEAVQQLQPPEDGLFLVRESARHPGDYVLCVSFGRDVIHYRVLHRDGHLTIDEAVFFCNLMDMVEHYSKDKGAICTKLVRPKRKHGTKSAEEELARAGWLLNLQHLTLGAQIGEGEFGAVLQGEYLGQKVAVKNIKCDVTAQAFLDETAVMTKMQHENLVRLLGVILHQGLYIVMEHVSKGNLVNFLRTRGRALVNTAQLLQFSLHVAEGMEYLESKKLVHRDLAARNILVSEDLVAKVSDFGLAKAERKGLDSSRLPVKWTAPEALKHGKFTSKSDVWSFGVLLWEVFSYGRAPYPKMSLKEVSEAVEKGYRMEPPEGCPGPVHVLMSSCWEAEPARRPPFRKLAEKLARELRSAGAPASVSGQDADGSTSPRSQEP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Methylation	----MAGRGSLVSWR ----CCCCCCCEEHH	23.22	-
11	Methylation	RGSLVSWRAFHGCDS CCCCEEHHHHCCCCC	21.62	-
18	Phosphorylation	RAFHGCDSAEELPRV HHHCCCCCHHHCCCC	41.47	28450419
18 (in isoform 2)	Phosphorylation	-	41.47	-
26	Phosphorylation	AEELPRVSPRFLRAW HHHCCCCCHHHHHHC	16.19	23401153
27 (in isoform 2)	Phosphorylation	-	28.34	-
31	Methylation	RVSPRFLRAWHPPPV CCCHHHHHHCCCCCC	32.68	-
75	Ubiquitination	AFRKGDVVTILEACE EEECCCEEEEEHHHC	3.07	21890473
76	Phosphorylation	FRKGDVVTILEACEN EECCCEEEEEHHHCC	21.50	-
85	Phosphorylation	LEACENKSWYRVKHH EHHHCCCCEEEEEEC	40.70	-
87	Phosphorylation	ACENKSWYRVKHHTS HHCCCCEEEEEECCC	16.78	-
116	Ubiquitination	EALSADPKLSLMPWF HHHCCCCCHHHCCCC	51.78	21890473
117	Ubiquitination	ALSADPKLSLMPWFH HHCCCCCHHHCCCCC	6.06	21890473
117 (in isoform 2)	Ubiquitination	-	6.06	-
118	Phosphorylation	LSADPKLSLMPWFHG HCCCCCHHHCCCCCC	28.84	27461979
217	Ubiquitination	PKRKHGTKSAEEELA CCCCCCCCCHHHHHH	52.87	-
308	Phosphorylation	YIVMEHVSKGNLVNF EEEEECCCCCCHHHH	36.43	-
333	Phosphorylation	TAQLLQFSLHVAEGM HHHHHHHHHHHHHCH	12.47	28387310
345	Phosphorylation	EGMEYLESKKLVHRD HCHHHHHHCCHHCHH	32.28	28387310
384	Phosphorylation	ERKGLDSSRLPVKWT HHCCCCCCCCCCCCC	37.19	24719451
441	Phosphorylation	SEAVEKGYRMEPPEG HHHHHHCCCCCCCCC	20.18	29496907
484	Phosphorylation	KLARELRSAGAPASV HHHHHHHHCCCCCCC	43.61	29396449
490	Phosphorylation	RSAGAPASVSGQDAD HHCCCCCCCCCCCCC	18.76	29396449
492	Phosphorylation	AGAPASVSGQDADGS CCCCCCCCCCCCCCC	28.39	29978859
499	Phosphorylation	SGQDADGSTSPRSQE CCCCCCCCCCCCCCC	26.68	23401153
500 (in isoform 2)	Phosphorylation	-	48.56	27251275
500	Phosphorylation	GQDADGSTSPRSQEP CCCCCCCCCCCCCCC	48.56	25159151
501	Phosphorylation	QDADGSTSPRSQEP- CCCCCCCCCCCCCC-	21.73	17525332
504	Phosphorylation	DGSTSPRSQEP---- CCCCCCCCCCC----	42.13	17525332

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	SMURF1	Q9HCE7	PMID:20804422

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MATK_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MATK_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NTRK1_HUMAN	NTRK1	physical	10329710
PAXI_HUMAN	PXN	physical	10080957
KIT_HUMAN	KIT	physical	7536744
SRC_HUMAN	SRC	physical	7530249

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501, AND MASSSPECTROMETRY.
PubMed: 19369195

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501, AND MASSSPECTROMETRY.
PubMed: 19413330

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501, AND MASSSPECTROMETRY.
PubMed: 18669648

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501, AND MASSSPECTROMETRY.
PubMed: 18691976

"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage.";
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
Science 316:1160-1166(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501 AND SER-504, ANDMASS SPECTROMETRY.
PubMed: 17525332

"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501, AND MASSSPECTROMETRY.
PubMed: 15302935