MARK4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MARK4_MOUSE
• UniProt AC: Q8CIP4
• Protein Name: MAP/microtubule affinity-regulating kinase 4
• Gene Name: Mark4
• Organism: Mus musculus (Mouse).
• Sequence Length: 752
• Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center . Cytoplasm, cytoskeleton, cilium axoneme . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm . Cell projection, dendrite . No
• Protein Description: Serine/threonine-protein kinase (By similarity). Phosphorylates the microtubule-associated protein MAPT/TAU (By similarity). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (By similarity). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (By similarity). Required for the initiation of axoneme extension during cilium assembly (By similarity). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (By similarity). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (By similarity). Reduces neuronal cell survival (By similarity). Plays a role in energy homeostasis by regulating satiety and metabolic rate. [PubMed: 22992738 Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway]
• Protein Sequence: MSSRTALAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYRLLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKEALTNQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGSSHNKGQRASSSTYHRQRRHSDFCGPSPAPLHPKRSPTSTGDTELKEERMPGRKASCSAVGSGSRGLPPSSPMVSSAHNPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGSERPSLLPNGKENSSGTSRVPPASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGSGSGGGVQNGPPASPTLAHEAAPLPSGRPRPTTNLFTKLTSKLTRRVTDEPERIGGPEVTSCHLPWDKTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLHGGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRTLVTRISNDLEL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSSRTALAP ------CCCCCCCCC	28.64	23527152
3	Phosphorylation	-----MSSRTALAPG -----CCCCCCCCCC	32.14	23527152
26	Phosphorylation	GTLGSGRSSDKGPSW CCCCCCCCCCCCCCC	46.55	23684622
45	Phosphorylation	LGARCRNSIASCPEE HHHHHHHCHHCCCCC	10.53	29899451
64	Phosphorylation	GNYRLLRTIGKGNFA CCCHHHEEECCCCCH	33.77	22324799
81	Phosphorylation	KLARHILTGREVAIK HHHHHHHCCCHHHEE	33.32	28059163
214	Phosphorylation	TLGSKLDTFCGSPPY ECCCCHHHCCCCCCC	31.12	25521595
218	Phosphorylation	KLDTFCGSPPYAAPE CHHHCCCCCCCCCHH	24.71	22942356
221	Phosphorylation	TFCGSPPYAAPELFQ HCCCCCCCCCHHHHC	20.84	25159016
300	Phosphorylation	LNPAKRCTLEQIMKD CCHHHCCCHHHHHHC	37.04	20139300
391	Phosphorylation	LARVRAPSDTTNGTS EEEEECCCCCCCCCC	46.75	30635358
393	Phosphorylation	RVRAPSDTTNGTSSS EEECCCCCCCCCCCC	26.88	30635358
412	Phosphorylation	HNKGQRASSSTYHRQ CCCCCCCCCCHHHHH	27.32	29899451
414	Phosphorylation	KGQRASSSTYHRQRR CCCCCCCCHHHHHHH	30.41	29899451
415	Phosphorylation	GQRASSSTYHRQRRH CCCCCCCHHHHHHHC	25.87	29899451
423	Phosphorylation	YHRQRRHSDFCGPSP HHHHHHCCCCCCCCC	30.48	26824392
438	Phosphorylation	APLHPKRSPTSTGDT CCCCCCCCCCCCCCH	38.36	25521595
440	Phosphorylation	LHPKRSPTSTGDTEL CCCCCCCCCCCCHHH	39.92	25521595
441	Phosphorylation	HPKRSPTSTGDTELK CCCCCCCCCCCHHHH	33.50	25619855
442	Phosphorylation	PKRSPTSTGDTELKE CCCCCCCCCCHHHHH	41.37	25619855
445	Phosphorylation	SPTSTGDTELKEERM CCCCCCCHHHHHHCC	44.56	29472430
458	Phosphorylation	RMPGRKASCSAVGSG CCCCCCCCCCCCCCC	16.19	25266776
464	Phosphorylation	ASCSAVGSGSRGLPP CCCCCCCCCCCCCCC	27.31	29899451
466	Phosphorylation	CSAVGSGSRGLPPSS CCCCCCCCCCCCCCC	25.52	29899451
494	Phosphorylation	IPERRKDSTSTPNNL CCHHHCCCCCCCCCC	27.56	25521595
496	Phosphorylation	ERRKDSTSTPNNLPP HHHCCCCCCCCCCCH	45.91	22871156
497	Phosphorylation	RRKDSTSTPNNLPPS HHCCCCCCCCCCCHH	30.08	25521595
511	Phosphorylation	SMMTRRNTYVCTERP HHHCCCCEEEEECCC	18.60	22322096
515	Phosphorylation	RRNTYVCTERPGSER CCCEEEEECCCCCCC	25.61	22322096
543	Phosphorylation	TSRVPPASPSSHSLA CCCCCCCCCCCCCCC	30.96	26824392
545	Phosphorylation	RVPPASPSSHSLAPP CCCCCCCCCCCCCCC	37.88	26824392
546	Phosphorylation	VPPASPSSHSLAPPS CCCCCCCCCCCCCCC	22.08	26643407
548	Phosphorylation	PASPSSHSLAPPSGE CCCCCCCCCCCCCCC	28.33	26643407
563	Phosphorylation	RSRLARGSTIRSTFH CHHHCCCCCCCCEEC	18.33	29899451
564	Phosphorylation	SRLARGSTIRSTFHG HHHCCCCCCCCEECC	24.19	29899451
567	Phosphorylation	ARGSTIRSTFHGGQV CCCCCCCCEECCCEE	31.41	27841257
568	Phosphorylation	RGSTIRSTFHGGQVR CCCCCCCEECCCEEE	15.14	29899451
581	Phosphorylation	VRDRRAGSGSGGGVQ EECCCCCCCCCCCCC	28.88	29472430
583	Phosphorylation	DRRAGSGSGGGVQNG CCCCCCCCCCCCCCC	35.64	29472430
594	Phosphorylation	VQNGPPASPTLAHEA CCCCCCCCCCCCCCC	24.96	29472430
596	Phosphorylation	NGPPASPTLAHEAAP CCCCCCCCCCCCCCC	33.80	29472430
606	Phosphorylation	HEAAPLPSGRPRPTT CCCCCCCCCCCCCCC	55.93	29899451
665	Phosphorylation	FPWSVKLTSSRPPEA CCCEEEECCCCCHHH	20.95	-
666	Phosphorylation	PWSVKLTSSRPPEAL CCEEEECCCCCHHHH	34.48	-
667	Phosphorylation	WSVKLTSSRPPEALM CEEEECCCCCHHHHH	43.50	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
214	T	Phosphorylation	Kinase	LKB1	Q9WTK7	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
29	K	Phosphorylation	Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation.	-
29	K	ubiquitylation	Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation.	-
214	T	Phosphorylation	Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MARK4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
1433F_MOUSE	Ywhah	physical	16959763
AAPK1_MOUSE	Prkaa1	genetic	23184942
AAPK2_MOUSE	Prkaa2	genetic	23184942

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.