dbPTM

MARK1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MARK1_MOUSE
UniProt AC	Q8VHJ5
Protein Name	Serine/threonine-protein kinase MARK1
Gene Name	Mark1 {ECO:0000312\|MGI:MGI:2664902}
Organism	Mus musculus (Mouse).
Sequence Length	795
Subcellular Localization	Cell membrane Peripheral membrane protein. Cytoplasm, cytoskeleton. Cytoplasm . Cell projection, dendrite . Appears to localize to an intracellular network..
Protein Description	Serine/threonine-protein kinase (By similarity). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (By similarity). Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3)..
Protein Sequence	MSARTPLPTVNERDTENHTSVDGYTETHIPPAKSSSRQNLPRCRNSITSATDEQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKCIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYSEPELDLSDAKRIDIMVTMGFARDEINDALVSQKYDEVMATYILLGRKPPEFEGGESLSSGSLCQRSRPSSDLNNSTLQSPAHLKVQRSISANQKQRRFSDHAGPSIPPAVSYTKRPQANSVESEQKEEWGKDTARRLGSTTVGSKSEVTASPLVGPDRKKSTASPSNNVYSGGSMARRNTYVCERSTDRYAALQNGRDSSLTEMSASSMSSAGSTVASAGPSARPRHQKSMSTSGHPIKVTLPTIKDGSEAYRPGAAQRVPAASPSAHSISASTPDRTRFPRGSSSRSTFHGEQLRERRSAAYNGPPASPSHETGAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRADTARGSSGDPKERDKDEGKEAKPRSLRFTWSMKTTSSMDPNDMLREIRKVLDANTCDYEQKERFLLFCVHGDARQDSLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSARTPLPT ------CCCCCCCCC	26.85	29514104
5	Phosphorylation	---MSARTPLPTVNE ---CCCCCCCCCCCC	28.97	-
35	Phosphorylation	HIPPAKSSSRQNLPR CCCCCCCCCCCCCHH	28.91	29899451
36	Phosphorylation	IPPAKSSSRQNLPRC CCCCCCCCCCCCHHH	45.00	29514104
46	Phosphorylation	NLPRCRNSITSATDE CCHHHHHCCCCCCCC	14.11	26370283
48	Phosphorylation	PRCRNSITSATDEQP HHHHHCCCCCCCCCC	16.48	29899451
49	Phosphorylation	RCRNSITSATDEQPH HHHHCCCCCCCCCCC	27.74	29899451
51	Phosphorylation	RNSITSATDEQPHIG HHCCCCCCCCCCCCC	39.58	29899451
168	Phosphorylation	AKFRQIVSAVQYCHQ HHHHHHHHHHHHHHH	25.09	-
172	Phosphorylation	QIVSAVQYCHQKCIV HHHHHHHHHHHHHCC	5.82	26487105
208	Phosphorylation	FGFSNEFTVGNKLDT CCCCCCEECCCCHHH	22.71	-
215	Phosphorylation	TVGNKLDTFCGSPPY ECCCCHHHCCCCCCC	31.12	25521595
219	Phosphorylation	KLDTFCGSPPYAAPE CHHHCCCCCCCCCHH	24.71	22942356
222	Phosphorylation	TFCGSPPYAAPELFQ HCCCCCCCCCHHHHC	20.84	25159016
298	Ubiquitination	LLVLNPIKRGSLEQI HHHHCCCCCCCHHHH	52.54	-
380	Phosphorylation	PEFEGGESLSSGSLC CCCCCCCCCCCCCCC	36.50	22324799
382	Phosphorylation	FEGGESLSSGSLCQR CCCCCCCCCCCCCCC	41.76	22324799
383	Phosphorylation	EGGESLSSGSLCQRS CCCCCCCCCCCCCCC	37.38	25521595
385	Phosphorylation	GESLSSGSLCQRSRP CCCCCCCCCCCCCCC	28.11	25521595
390	Phosphorylation	SGSLCQRSRPSSDLN CCCCCCCCCCCCCCC	23.75	25521595
393	Phosphorylation	LCQRSRPSSDLNNST CCCCCCCCCCCCCCC	35.06	25521595
394	Phosphorylation	CQRSRPSSDLNNSTL CCCCCCCCCCCCCCC	49.12	25521595
399	Phosphorylation	PSSDLNNSTLQSPAH CCCCCCCCCCCCHHH	29.44	25521595
400	Phosphorylation	SSDLNNSTLQSPAHL CCCCCCCCCCCHHHH	30.82	29899451
403	Phosphorylation	LNNSTLQSPAHLKVQ CCCCCCCCHHHHHHH	27.45	25521595
412	Phosphorylation	AHLKVQRSISANQKQ HHHHHHHHCCCHHHC	12.10	22324799
414	Phosphorylation	LKVQRSISANQKQRR HHHHHHCCCHHHCCC	23.55	25521595
423	Phosphorylation	NQKQRRFSDHAGPSI HHHCCCCCCCCCCCC	27.03	22817900
429	Phosphorylation	FSDHAGPSIPPAVSY CCCCCCCCCCCCCCC	46.97	29472430
444	Phosphorylation	TKRPQANSVESEQKE CCCCCCCCCCHHHHH	30.22	25521595
447	Phosphorylation	PQANSVESEQKEEWG CCCCCCCHHHHHHHC	43.08	22324799
457	Phosphorylation	KEEWGKDTARRLGST HHHHCHHHHHHHCCC	26.08	22324799
463	Phosphorylation	DTARRLGSTTVGSKS HHHHHHCCCCCCCCC	26.04	22324799
464	Phosphorylation	TARRLGSTTVGSKSE HHHHHCCCCCCCCCE	24.57	29899451
465	Phosphorylation	ARRLGSTTVGSKSEV HHHHCCCCCCCCCEE	25.27	22324799
468	Phosphorylation	LGSTTVGSKSEVTAS HCCCCCCCCCEEEEC	28.77	29899451
470	Phosphorylation	STTVGSKSEVTASPL CCCCCCCCEEEECCC	38.71	23527152
473	Phosphorylation	VGSKSEVTASPLVGP CCCCCEEEECCCCCC	19.84	29514104
475	Phosphorylation	SKSEVTASPLVGPDR CCCEEEECCCCCCCC	14.92	23527152
485	Phosphorylation	VGPDRKKSTASPSNN CCCCCCCCCCCCCCC	32.04	23527152
486	Phosphorylation	GPDRKKSTASPSNNV CCCCCCCCCCCCCCC	39.78	25521595
488	Phosphorylation	DRKKSTASPSNNVYS CCCCCCCCCCCCCCC	29.02	23527152
490	Phosphorylation	KKSTASPSNNVYSGG CCCCCCCCCCCCCCC	38.24	29899451
494	Phosphorylation	ASPSNNVYSGGSMAR CCCCCCCCCCCCCHH	12.06	29514104
495	Phosphorylation	SPSNNVYSGGSMARR CCCCCCCCCCCCHHC	32.18	29899451
498	Phosphorylation	NNVYSGGSMARRNTY CCCCCCCCCHHCCCE	16.88	29899451
504	Phosphorylation	GSMARRNTYVCERST CCCHHCCCEEECCCH	18.60	25521595
505	Phosphorylation	SMARRNTYVCERSTD CCHHCCCEEECCCHH	13.47	29514104
523	Phosphorylation	ALQNGRDSSLTEMSA HHHCCCCCCCHHHCH	26.36	29899451
524	Phosphorylation	LQNGRDSSLTEMSAS HHCCCCCCCHHHCHH	43.53	29899451
546	Phosphorylation	TVASAGPSARPRHQK CHHCCCCCCCCCCCC	35.67	-
554	Phosphorylation	ARPRHQKSMSTSGHP CCCCCCCCCCCCCCC	16.05	29899451
556	Phosphorylation	PRHQKSMSTSGHPIK CCCCCCCCCCCCCCE	26.89	22817900
557	Phosphorylation	RHQKSMSTSGHPIKV CCCCCCCCCCCCCEE	30.04	26804993
558	Phosphorylation	HQKSMSTSGHPIKVT CCCCCCCCCCCCEEE	28.00	29472430
565	Phosphorylation	SGHPIKVTLPTIKDG CCCCCEEEECCCCCC	23.24	26804993
568	Phosphorylation	PIKVTLPTIKDGSEA CCEEEECCCCCCCCC	43.71	26804993
588	Phosphorylation	AQRVPAASPSAHSIS HHCCCCCCCCCCCCC	22.84	29514104
590	Phosphorylation	RVPAASPSAHSISAS CCCCCCCCCCCCCCC	35.80	29899451
593	Phosphorylation	AASPSAHSISASTPD CCCCCCCCCCCCCCC	20.25	22817900
595	Phosphorylation	SPSAHSISASTPDRT CCCCCCCCCCCCCCC	21.12	29899451
597	Phosphorylation	SAHSISASTPDRTRF CCCCCCCCCCCCCCC	33.41	29899451
598	Phosphorylation	AHSISASTPDRTRFP CCCCCCCCCCCCCCC	29.00	29899451
609	Phosphorylation	TRFPRGSSSRSTFHG CCCCCCCCCCCCCCH	32.37	29514104
612	Phosphorylation	PRGSSSRSTFHGEQL CCCCCCCCCCCHHHH	37.06	22817900
613	Phosphorylation	RGSSSRSTFHGEQLR CCCCCCCCCCHHHHH	20.67	22817900
624	Phosphorylation	EQLRERRSAAYNGPP HHHHHHHHHHHCCCC	24.38	29514104
627	Phosphorylation	RERRSAAYNGPPASP HHHHHHHHCCCCCCC	21.99	29514104
633	Phosphorylation	AYNGPPASPSHETGA HHCCCCCCCCHHCCC	32.48	30372032
635	Phosphorylation	NGPPASPSHETGAFA CCCCCCCCHHCCCCC	31.31	29899451
638	Phosphorylation	PASPSHETGAFAHAR CCCCCHHCCCCCHHH	28.52	29899451
648	Phosphorylation	FAHARRGTSTGIISK CCHHHCCCCCCHHHH	22.70	29899451
649	Phosphorylation	AHARRGTSTGIISKI CHHHCCCCCCHHHHH	27.71	22324799
649	O-linked_Glycosylation	AHARRGTSTGIISKI CHHHCCCCCCHHHHH	27.71	22645316
650	Phosphorylation	HARRGTSTGIISKIT HHHCCCCCCHHHHHH	32.03	22324799
666	Phosphorylation	KFVRRDPSEGEASGR HHCCCCCCCCCCCCC	63.62	25521595
671	Phosphorylation	DPSEGEASGRADTAR CCCCCCCCCCCCCCC	25.22	25521595
680	Phosphorylation	RADTARGSSGDPKER CCCCCCCCCCCHHHC	26.12	29899451
699	Phosphorylation	GKEAKPRSLRFTWSM CCCCCCCCEEEEEEC	32.21	-
705	Phosphorylation	RSLRFTWSMKTTSSM CCEEEEEECCCCCCC	13.82	22006019
766	Phosphorylation	VCKLPRLSLNGVRFK HHCCCCEEECCEEEE	22.52	21183079
783	Ubiquitination	SGTSIAFKNIASKIA CCCHHHHHHHHHHHH	38.12	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
215	T	Phosphorylation	Kinase	LKB1	Q9WTK7	Uniprot
215	T	Phosphorylation	Kinase	TAOK1	Q5F2E8	Uniprot
219	S	Phosphorylation	Kinase	GSK3-BETA	Q9WV60	Uniprot
613	T	Phosphorylation	Kinase	PRKCZ	Q02956	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
215	T	Phosphorylation	-
Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.
215	T	Phosphorylation	-
Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules.
219	S	Phosphorylation	-
Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity.
613	T	Phosphorylation	-
Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MARK1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of MARK1_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MARK1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394; SER-403 ANDSER-666, AND MASS SPECTROMETRY.	16452087 [Abstract]

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