dbPTM

M4K4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	M4K4_MOUSE
UniProt AC	P97820
Protein Name	Mitogen-activated protein kinase kinase kinase kinase 4
Gene Name	Map4k4
Organism	Mus musculus (Mouse).
Sequence Length	1233
Subcellular Localization	Cytoplasm.
Protein Description	Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322 (By similarity)..
Protein Sequence	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTVTAAIKVMDVTEDEEEEITLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGGPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQQPPPPQQQDRSKPSFHAPEPKPHYDPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGGGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSPRQESAAKKPDDKKEVFRSLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSPNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELLHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCGRNDKVFFSSVRSGGSSQVYFMTLGRTSLLSW

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MANDSPAKS ------CCCCCCCHH	39.58	-
5	Phosphorylation	---MANDSPAKSLVD ---CCCCCCCHHHEE	26.94	-
16	Phosphorylation	SLVDIDLSSLRDPAG HHEEECHHHCCCCCC	24.47	25159016
17	Phosphorylation	LVDIDLSSLRDPAGI HEEECHHHCCCCCCE	34.42	25159016
123	Phosphorylation	VKNTKGNTLKEDWIA HHCCCCCCCCHHHHH	48.12	-
131	Phosphorylation	LKEDWIAYISREILR CCHHHHHHHHHHHHH	7.12	-
133	Phosphorylation	EDWIAYISREILRGL HHHHHHHHHHHHHHH	15.93	-
174	Phosphorylation	KLVDFGVSAQLDRTV EEEEECCEEECCCCC	15.63	23140645
180	Phosphorylation	VSAQLDRTVGRRNTF CEEECCCCCCCCCCC	27.29	22324799
186	Phosphorylation	RTVGRRNTFIGTPYW CCCCCCCCCCCCCCC	18.15	26643407
190	Phosphorylation	RRNTFIGTPYWMAPE CCCCCCCCCCCCCCC	14.37	23984901
192	Phosphorylation	NTFIGTPYWMAPEVI CCCCCCCCCCCCCEE	13.98	23984901
318	Phosphorylation	KRGEKDETEYEYSGS HCCCCCCCCCCCCCC	54.74	25293948
320	Phosphorylation	GEKDETEYEYSGSEE CCCCCCCCCCCCCHH	27.82	25293948
322	Phosphorylation	KDETEYEYSGSEEEE CCCCCCCCCCCHHHH	19.89	25293948
323	Phosphorylation	DETEYEYSGSEEEEE CCCCCCCCCCHHHHC	24.73	23737553
325	Phosphorylation	TEYEYSGSEEEEEEV CCCCCCCCHHHHCCC	35.57	23737553
340	Phosphorylation	PEQEGEPSSIVNVPG CCCCCCCCCCEECCC	28.52	23737553
341	Phosphorylation	EQEGEPSSIVNVPGE CCCCCCCCCEECCCC	41.07	23737553
349	Phosphorylation	IVNVPGESTLRRDFL CEECCCCCHHHHHHH	38.36	23737553
350	Phosphorylation	VNVPGESTLRRDFLR EECCCCCHHHHHHHH	21.71	23737553
466	Phosphorylation	RVEREQEYIRRQLEE HHHHHHHHHHHHHHH	10.22	29514104
517	Phosphorylation	QQDRSKPSFHAPEPK CCCCCCCCCCCCCCC	33.14	29899451
518	Phosphorylation	QDRSKPSFHAPEPKP CCCCCCCCCCCCCCC	8.48	24719451
527	Phosphorylation	APEPKPHYDPADRAR CCCCCCCCCHHHHHH	31.95	29514104
543	Phosphorylation	VQWSHLASLKNNVSP HHHHHHHHHCCCCCC	46.35	21743459
549	Phosphorylation	ASLKNNVSPVSRSHS HHHCCCCCCCCCCCC	23.00	24899341
552	Phosphorylation	KNNVSPVSRSHSFSD CCCCCCCCCCCCCCC	31.30	26824392
554	Phosphorylation	NVSPVSRSHSFSDPS CCCCCCCCCCCCCCC	18.90	23527152
556	Phosphorylation	SPVSRSHSFSDPSPK CCCCCCCCCCCCCCC	27.91	25521595
558	Phosphorylation	VSRSHSFSDPSPKFA CCCCCCCCCCCCCHH	52.18	27742792
561	Phosphorylation	SHSFSDPSPKFAHHH CCCCCCCCCCHHHHH	45.79	26824392
604	Phosphorylation	TQKAWSRSDSDEVPP CCCHHCCCCCCCCCC	35.63	30372032
606	Phosphorylation	KAWSRSDSDEVPPRV CHHCCCCCCCCCCCC	36.60	30372032
617	Phosphorylation	PPRVPVRTTSRSPVL CCCCCCCCCCCCCCC	29.38	25177544
618	Phosphorylation	PRVPVRTTSRSPVLS CCCCCCCCCCCCCCC	16.16	26060331
619	Phosphorylation	RVPVRTTSRSPVLSR CCCCCCCCCCCCCCC	30.10	26824392
621	Phosphorylation	PVRTTSRSPVLSRRD CCCCCCCCCCCCCCC	21.08	25521595
625	Phosphorylation	TSRSPVLSRRDSPLQ CCCCCCCCCCCCCCC	26.04	25159016
629	Phosphorylation	PVLSRRDSPLQGGGQ CCCCCCCCCCCCCCC	26.21	25521595
630	Phosphorylation	VLSRRDSPLQGGGQQ CCCCCCCCCCCCCCC	32.75	24719451
639	Phosphorylation	QGGGQQNSQAGQRNS CCCCCCCCCCCCCCC	19.64	26643407
646	Phosphorylation	SQAGQRNSTSSIEPR CCCCCCCCCCCCCHH	31.68	25521595
647	Phosphorylation	QAGQRNSTSSIEPRL CCCCCCCCCCCCHHH	30.00	25168779
648	Phosphorylation	AGQRNSTSSIEPRLL CCCCCCCCCCCHHHH	29.19	27087446
649	Phosphorylation	GQRNSTSSIEPRLLW CCCCCCCCCCHHHHH	30.93	27087446
668	Phosphorylation	KLVPRPGSGSSSGSS HHCCCCCCCCCCCCC	37.88	25619855
670	Phosphorylation	VPRPGSGSSSGSSNS CCCCCCCCCCCCCCC	24.03	25619855
671	Phosphorylation	PRPGSGSSSGSSNSG CCCCCCCCCCCCCCC	42.09	25619855
672	Phosphorylation	RPGSGSSSGSSNSGS CCCCCCCCCCCCCCC	44.37	25619855
674	Phosphorylation	GSGSSSGSSNSGSQP CCCCCCCCCCCCCCC	28.44	25619855
675	Phosphorylation	SGSSSGSSNSGSQPG CCCCCCCCCCCCCCC	38.24	25619855
677	Phosphorylation	SSSGSSNSGSQPGSH CCCCCCCCCCCCCCC	41.59	25619855
679	Phosphorylation	SGSSNSGSQPGSHPG CCCCCCCCCCCCCCC	32.78	25619855
683	Phosphorylation	NSGSQPGSHPGSQSG CCCCCCCCCCCCCCC	32.94	25619855
687	Phosphorylation	QPGSHPGSQSGSGER CCCCCCCCCCCCCCC	26.14	25619855
689	Phosphorylation	GSHPGSQSGSGERFR CCCCCCCCCCCCCEE	36.30	25619855
691	Phosphorylation	HPGSQSGSGERFRVR CCCCCCCCCCCEEEE	42.60	27087446
699	Phosphorylation	GERFRVRSSSKSEGS CCCEEEECCCCCCCC	35.79	25266776
700	Phosphorylation	ERFRVRSSSKSEGSP CCEEEECCCCCCCCC	31.08	25266776
701	Phosphorylation	RFRVRSSSKSEGSPS CEEEECCCCCCCCCC	41.25	24453211
702	Phosphorylation	FRVRSSSKSEGSPSP EEEECCCCCCCCCCH	55.25	24719451
703	Phosphorylation	RVRSSSKSEGSPSPR EEECCCCCCCCCCHH	49.49	30635358
706	Phosphorylation	SSSKSEGSPSPRQES CCCCCCCCCCHHHHH	20.39	29899451
708	Phosphorylation	SKSEGSPSPRQESAA CCCCCCCCHHHHHHC	34.12	21183079
785	Phosphorylation	EGADDSTSGPEDTRA CCCCCCCCCHHHHCC	57.19	-
794	Phosphorylation	PEDTRAASSPNLSNG HHHHCCCCCCCCCCC	45.39	26824392
795	Phosphorylation	EDTRAASSPNLSNGE HHHCCCCCCCCCCCC	17.06	25521595
799	Phosphorylation	AASSPNLSNGETESV CCCCCCCCCCCCCCE	49.80	25521595
803	Phosphorylation	PNLSNGETESVKTMI CCCCCCCCCCEEEEE	34.65	25619855
805	Phosphorylation	LSNGETESVKTMIVH CCCCCCCCEEEEEEE	36.01	25619855
817	Phosphorylation	IVHDDVESEPAMTPS EEECCCCCCCCCCCC	49.05	-
822	Phosphorylation	VESEPAMTPSKEGTL CCCCCCCCCCCCCEE	26.90	29550500
824	Phosphorylation	SEPAMTPSKEGTLIV CCCCCCCCCCCEEEE	33.89	25521595
825	Acetylation	EPAMTPSKEGTLIVR CCCCCCCCCCEEEEE	62.46	19861063
828	Phosphorylation	MTPSKEGTLIVRQTQ CCCCCCCEEEEEECC	17.88	27180971
834	Phosphorylation	GTLIVRQTQSASSTL CEEEEEECCCCCHHH	17.58	28066266
836	Phosphorylation	LIVRQTQSASSTLQK EEEEECCCCCHHHHH	33.24	26824392
838	Phosphorylation	VRQTQSASSTLQKHK EEECCCCCHHHHHCC	29.15	28066266
839	Phosphorylation	RQTQSASSTLQKHKS EECCCCCHHHHHCCC	32.62	26824392
840	Phosphorylation	QTQSASSTLQKHKSS ECCCCCHHHHHCCCC	30.61	26824392
846	Phosphorylation	STLQKHKSSSSFTPF HHHHHCCCCCCCCCC	34.86	23684622
847	Phosphorylation	TLQKHKSSSSFTPFI HHHHCCCCCCCCCCC	34.50	26643407
848	Phosphorylation	LQKHKSSSSFTPFID HHHCCCCCCCCCCCC	36.24	25266776
849	Phosphorylation	QKHKSSSSFTPFIDP HHCCCCCCCCCCCCH	34.78	29514104
851	Phosphorylation	HKSSSSFTPFIDPRL CCCCCCCCCCCCHHH	21.16	26643407
862	Phosphorylation	DPRLLQISPSSGTTV CHHHEEECCCCCCEE	13.03	26643407
864	Phosphorylation	RLLQISPSSGTTVTS HHEEECCCCCCEEEE	34.31	26643407
865	Phosphorylation	LLQISPSSGTTVTSV HEEECCCCCCEEEEE	43.47	26643407
868	Phosphorylation	ISPSSGTTVTSVVGF ECCCCCCEEEEEEEE	25.88	25338131
870	Phosphorylation	PSSGTTVTSVVGFSC CCCCCEEEEEEEEEC	17.45	21183079
871	Phosphorylation	SSGTTVTSVVGFSCD CCCCEEEEEEEEECC	15.52	21183079
894	Phosphorylation	QDPTRKGSVVNVNPT CCCCCCCCEEECCCC	26.40	26824392
901	Phosphorylation	SVVNVNPTNTRPQSD CEEECCCCCCCCCCC	43.98	25619855
903	Phosphorylation	VNVNPTNTRPQSDTP EECCCCCCCCCCCCH	46.32	25619855
907	Phosphorylation	PTNTRPQSDTPEIRK CCCCCCCCCCHHHHH	46.24	25619855
909	Phosphorylation	NTRPQSDTPEIRKYK CCCCCCCCHHHHHHH	28.24	25619855
989	Phosphorylation	KLRVYYLSWLRNKIL HHHHEHHHHHHHHHH	14.14	21183079
1020	Phosphorylation	DLEGCVHYKVVKYER CCCCCEEEEEEEHHH	5.77	-
1218	Phosphorylation	SVRSGGSSQVYFMTL EEECCCCCEEEEEEE	27.26	29514104
1224	Phosphorylation	SSQVYFMTLGRTSLL CCEEEEEEECCCHHC	19.03	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of M4K4_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of M4K4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of M4K4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of M4K4_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of M4K4_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621 AND SER-629, ANDMASS SPECTROMETRY.	19367708 [Abstract]
"Phosphoproteomic analysis of the developing mouse brain."; Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.; Mol. Cell. Proteomics 3:1093-1101(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-795, AND MASSSPECTROMETRY.	15345747 [Abstract]