M3K14_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: M3K14_MOUSE
• UniProt AC: Q9WUL6
• Protein Name: Mitogen-activated protein kinase kinase kinase 14
• Gene Name: Map3k14
• Organism: Mus musculus (Mouse).
• Sequence Length: 942
• Subcellular Localization: Cytoplasm.
• Protein Description: Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner..
• Protein Sequence: MAVMEVACPGTPGSAVGQQKELAKAKEKTQSLGKKQSCIFKLEAVEKSPVFCGKWEILNDVITKGTAKDGSEGGPPAISIIAQAECENSQEFSPTFSERIFIAGSQQYSQSESLDQIPNNVAHATEGKMARVCRRGKRHGKARKKRRKKRSKSLAQAGVALAKPLPRTPEQESCTIPVQEDESPLGNLYARNVSQFTKPLGGPGLGHLCFKKQDEGLRPVLPRPELHKLISPLQCLNHVWKLHHPQATGPRPHPTHPFPYSGMPHPFPFYPLEPWKPYMLDSAVLDKLAGVSGQRPLPGPPHLSQLAHGDSQKPLPGPHLESSCPSRGALEKVPVEEYLVHALQGSVSSGQAHSLASLAKTWSSGSAKLQRLGPETEDNEGVLLTEKLKPVDYEYREEVHWMTHQPRVGRGSFGEVHRMKDKQTGFQCAVKKVRLEVFRVEELVACAGLSSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLIKQMGCLPEDRALYYLGQALEGLEYLHTRRILHGDVKADNVLLSSDGSRAALCDFGHALCLQPDGLGKSLLTGDYIPGTETHMAPEVVMGKPCDAKVDIWSSCCMMLHMLNGCHPWTQYFRGPLCLKIASEPPPIREIPPSCAPLTAQAIQEGLRKEPVHRASAMELRRKVGKALQEVGGLKSPWKGEYKEPRPPPQDQATCHQTLPTPPRENPPAKANTDGAPEPQPPLPPEPPEPSKAPALNLSKEESGTWEPLPLSSLDPATAKGPSFPDRRATLPELELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWNSQAEARTCSCSTALARGRPTDIPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPATAFSLVTKDGQPVCYDMEVPDSGIDLQCTLAPDGSFAWTWRVKHGQLENRP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
48	Phosphorylation	KLEAVEKSPVFCGKW EEEECCCCCCCCCCC	16.88	-
89	Phosphorylation	AQAECENSQEFSPTF EEEECCCCCCCCCCH	14.59	-
113	Phosphorylation	QQYSQSESLDQIPNN CCCCCCCCHHHCCCC	42.36	-
153	Phosphorylation	RRKKRSKSLAQAGVA HHHHHHHHHHHHHHH	30.13	-
212	Ubiquitination	LGHLCFKKQDEGLRP CCCEEEECCCCCCCC	42.61	-
349	Phosphorylation	ALQGSVSSGQAHSLA HHHCCCCCCCHHHHH	32.54	-
354	Phosphorylation	VSSGQAHSLASLAKT CCCCCHHHHHHHHHH	28.75	-
357	Phosphorylation	GQAHSLASLAKTWSS CCHHHHHHHHHHHHC	33.87	-
412	Phosphorylation	QPRVGRGSFGEVHRM CCCCCCCCCCCCCCC	28.81	-
561	Phosphorylation	TGDYIPGTETHMAPE CCCCCCCCCCCCCCC	32.15	-
690	Phosphorylation	TCHQTLPTPPRENPP CCCCCCCCCCCCCCC	49.38	-
728	Phosphorylation	KAPALNLSKEESGTW CCCCCCCCCCCCCCC	36.63	25367039
732	Phosphorylation	LNLSKEESGTWEPLP CCCCCCCCCCCCCCC	42.48	25367039
734	Phosphorylation	LSKEESGTWEPLPLS CCCCCCCCCCCCCHH	35.61	25367039
741	Phosphorylation	TWEPLPLSSLDPATA CCCCCCHHHCCCCCC	27.24	25367039
742	Phosphorylation	WEPLPLSSLDPATAK CCCCCHHHCCCCCCC	44.46	25367039
747	Phosphorylation	LSSLDPATAKGPSFP HHHCCCCCCCCCCCC	34.00	25367039
752	Phosphorylation	PATAKGPSFPDRRAT CCCCCCCCCCCHHCC	59.07	23737553
799	Phosphorylation	CLSIDSLSLSDDSEK HHHCCCCCCCCCCCC	29.76	27149854
809	Phosphorylation	DDSEKNPSKASQSSR CCCCCCCCHHCHHHH	50.69	-
812	Phosphorylation	EKNPSKASQSSRDTL CCCCCHHCHHHHHHH	34.04	-
815	Phosphorylation	PSKASQSSRDTLSSG CCHHCHHHHHHHHHC	26.91	-
820	Phosphorylation	QSSRDTLSSGVHSWN HHHHHHHHHCCCCCC	27.43	-
821	Phosphorylation	SSRDTLSSGVHSWNS HHHHHHHHCCCCCCC	48.20	-
862	Phosphorylation	GVKVQIQSLNGEHLH CEEEEEEECCCCCEE	25.98	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
48	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
89	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
113	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
153	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
349	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
354	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
357	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
690	T	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
728	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
752	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
820	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
821	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
862	S	Phosphorylation	Kinase	TBK1	Q9UHD2	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	Traf2	P39429	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	Traf3	Q60803	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
48	K	ubiquitylation	Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination.	-
561	T	Phosphorylation	Phosphorylation at Thr-561 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination.	-
561	T	ubiquitylation	Phosphorylation at Thr-561 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of M3K14_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HSP7C_MOUSE	Hspa8	physical	25792747
CHIP_MOUSE	Stub1	physical	25792747

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.