KPB1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KPB1_HUMAN
• UniProt AC: P46020
• Protein Name: Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
• Gene Name: PHKA1
• Organism: Homo sapiens (Human).
• Sequence Length: 1223
• Subcellular Localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side .
• Protein Description: Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin..
• Protein Sequence: MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYKEALEAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKTILKDKGIYVETIAEVYPIRVQPARILSHIYSSLGCNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARVQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDNYDYLESGNWMNDYDSTSHARCGDEVARYLDHLLAHTAPHPKLAPTSQKGGLDRFQAAVQTTCDLMSLVTKAKELHVQNVHMYLPTKLFQASRPSFNLLDSPHPRQENQVPSVRVEIHLPRDQSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWNTELYNERSATVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSISAESQSPGTSMTPSSGSFPSAYDQQSSKDSRQGQWQRRRRLDGALNRVPVGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MRSRSNSGVR -----CCCCCCCCCC	52.50	26074081
5	Phosphorylation	---MRSRSNSGVRLD ---CCCCCCCCCCCC	36.27	23927012
7	Phosphorylation	-MRSRSNSGVRLDGY -CCCCCCCCCCCCHH	39.65	23401153
14	Phosphorylation	SGVRLDGYARLVQQT CCCCCCHHHHHHHHH	6.55	23403867
73	Ubiquitination	DRDEDKAKAYELEQS CCCHHHHHHHHHHHH	58.19	29967540
103	Ubiquitination	VDKVESFKYSQSTKD CCCHHHCCCCCCCCH	53.95	27667366
109	Ubiquitination	FKYSQSTKDSLHAKY CCCCCCCCHHHHHEE	51.03	29967540
111	Phosphorylation	YSQSTKDSLHAKYNT CCCCCCHHHHHEECC	24.50	28857561
115	Ubiquitination	TKDSLHAKYNTKTCA CCHHHHHEECCCCCE	28.43	29967540
200	Phosphorylation	GISELNASSVGMAKA CHHHCCHHHHHHHHH	25.20	21082442
201	Phosphorylation	ISELNASSVGMAKAA HHHCCHHHHHHHHHH	21.93	21082442
312	Phosphorylation	KEDPNRLYYEPAELK CCCCCCCCCCHHHHH	11.53	29978859
313	Phosphorylation	EDPNRLYYEPAELKL CCCCCCCCCHHHHHH	21.74	29978859
361	Ubiquitination	ALEAVLIKGKNGVPL HHHHHHHCCCCCCCC	60.72	29967540
405	Phosphorylation	LPHMWGQSLYILGSL CCCHHHCHHHHHHHH	21.14	-
407	Phosphorylation	HMWGQSLYILGSLMA CHHHCHHHHHHHHHH	10.19	-
431	Phosphorylation	DPLNRRFSTVPKPDV CCCCCCCCCCCCCCE	27.04	-
432	Phosphorylation	PLNRRFSTVPKPDVV CCCCCCCCCCCCCEE	37.81	-
435 (in isoform 2)	Ubiquitination	-	49.90	21906983
435 (in isoform 1)	Ubiquitination	-	49.90	21906983
435	Ubiquitination	RRFSTVPKPDVVVQV CCCCCCCCCCEEEEE	49.90	21906983
452	Ubiquitination	LAETEEIKTILKDKG EEEHHHHHHHHHHCC	32.56	2190698
452 (in isoform 2)	Ubiquitination	-	32.56	21906983
452 (in isoform 1)	Ubiquitination	-	32.56	21906983
453	Phosphorylation	AETEEIKTILKDKGI EEHHHHHHHHHHCCE	36.88	-
469	Phosphorylation	VETIAEVYPIRVQPA EEEEEEEECCCCCHH	5.62	-
511	Phosphorylation	VLGTSKLYDIRKTIF EECCCCHHHHHHHHE	17.04	-
549	Phosphorylation	MLRTDLSYLCSRWRM HHHHCHHHHHHHHHC	21.01	16493040
561	Phosphorylation	WRMTGQPTITFPISH HHCCCCCEEEEECCH	25.43	18669648
563	Phosphorylation	MTGQPTITFPISHSM CCCCCEEEEECCHHH	26.32	18669648
581	Phosphorylation	DGTSLNSSILAALRK CCCCCCHHHHHHHHH	22.13	18669648
629	Phosphorylation	GPEGKLYSEDYDDNY CCCCCCCCCCCCCCC	34.13	-
636	Phosphorylation	SEDYDDNYDYLESGN CCCCCCCCCCHHCCC	16.98	-
638	Phosphorylation	DYDDNYDYLESGNWM CCCCCCCCHHCCCCC	11.29	-
648	Phosphorylation	SGNWMNDYDSTSHAR CCCCCCCCCCCCCCC	13.54	-
676	Ubiquitination	AHTAPHPKLAPTSQK HHCCCCCCCCCCCCC	55.00	29967540
683	Ubiquitination	KLAPTSQKGGLDRFQ CCCCCCCCCCHHHHH	55.50	32015554
717	Phosphorylation	HVQNVHMYLPTKLFQ CCCCEEEECCCHHHH	8.47	-
726	Phosphorylation	PTKLFQASRPSFNLL CCHHHHCCCCCCCCC	33.66	28857561
729	Phosphorylation	LFQASRPSFNLLDSP HHHCCCCCCCCCCCC	26.17	28857561
735	Phosphorylation	PSFNLLDSPHPRQEN CCCCCCCCCCCCCCC	25.69	25159151
758	Phosphorylation	IHLPRDQSGEVDFKA EECCCCCCCCCCHHH	41.01	23911959
777	Ubiquitination	LKETSSLQEQADILY HHHCCCHHHHHHHHH	42.58	29967540
811	Phosphorylation	ATVRELLTELYGKVG HHHHHHHHHHHCHHH	36.25	23312004
814	Phosphorylation	RELLTELYGKVGEIR HHHHHHHHCHHHHHH	14.52	23312004
816	Ubiquitination	LLTELYGKVGEIRHW HHHHHHCHHHHHHHH	33.25	-
836	Ubiquitination	ISGILRKKVEALDEA HHHHHHHHHHHHHHH	38.58	29967540
906	Ubiquitination	VYLAMYMRTQPGLFA HHHHHHHHCCCCHHH	16.84	32015554
926	Phosphorylation	RIGLIIQVMATELAH HHHHHHHHHHHHHHH	1.66	32645325
934	Phosphorylation	MATELAHSLRCSAEE HHHHHHHHCCCCHHH	16.35	25332170
937	Ubiquitination	ELAHSLRCSAEEATE HHHHHCCCCHHHHHH	5.69	32015554
943	Phosphorylation	RCSAEEATEGLMNLS CCCHHHHHHHHHCCC	33.59	-
947	Ubiquitination	EEATEGLMNLSPSAM HHHHHHHHCCCHHHH	7.53	32015554
948 (in isoform 3)	Phosphorylation	-	42.27	27732954
952	Phosphorylation	GLMNLSPSAMKNLLH HHHCCCHHHHHHHHH	37.30	25332170
953 (in isoform 3)	Phosphorylation	-	19.16	27732954
956 (in isoform 3)	Phosphorylation	-	34.45	27732954
957 (in isoform 3)	Phosphorylation	-	3.93	27732954
959 (in isoform 3)	Phosphorylation	-	14.76	27732954
961 (in isoform 3)	Phosphorylation	-	1.97	25159151
962 (in isoform 3)	Phosphorylation	-	4.85	25159151
963	Phosphorylation	NLLHHILSGKEFGVE HHHHHHHHCCCCCCC	47.41	24719451
964 (in isoform 3)	Phosphorylation	-	30.02	25159151
965	Ubiquitination	LHHILSGKEFGVERS HHHHHHCCCCCCCCC	46.63	32015554
972	Phosphorylation	KEFGVERSVRPTDSN CCCCCCCCCCCCCCC	14.69	25159151
976	Phosphorylation	VERSVRPTDSNVSPA CCCCCCCCCCCCCCC	41.31	28176443
978	Phosphorylation	RSVRPTDSNVSPAIS CCCCCCCCCCCCCEE	41.42	28176443
981	Phosphorylation	RPTDSNVSPAISIHE CCCCCCCCCCEEHEE	16.92	28176443
985	Phosphorylation	SNVSPAISIHEIGAV CCCCCCEEHEEECCC	21.72	25159151
995	Phosphorylation	EIGAVGATKTERTGI EECCCCCCCCCCCCC	32.35	29978859
996	Ubiquitination	IGAVGATKTERTGIM ECCCCCCCCCCCCCH	48.10	32015554
997	Phosphorylation	GAVGATKTERTGIMQ CCCCCCCCCCCCCHH	26.75	23312004
1000	O-linked_Glycosylation	GATKTERTGIMQLKS CCCCCCCCCCHHHHH	25.48	30379171
1006	Ubiquitination	RTGIMQLKSEIKQVE CCCCHHHHHHHEEEE	29.20	32015554
1007	Phosphorylation	TGIMQLKSEIKQVEF CCCHHHHHHHEEEEE	53.82	28857561
1007 (in isoform 2)	Phosphorylation	-	53.82	27732954
1012 (in isoform 2)	Phosphorylation	-	6.79	27732954
1015 (in isoform 2)	Phosphorylation	-	25.73	27732954
1016 (in isoform 2)	Phosphorylation	-	37.80	27732954
1018 (in isoform 2)	Phosphorylation	-	16.82	27732954
1018	Phosphorylation	QVEFRRLSISAESQS EEEEEEEEEEEECCC	16.82	19764811
1020	Phosphorylation	EFRRLSISAESQSPG EEEEEEEEEECCCCC	23.70	19764811
1020 (in isoform 2)	Phosphorylation	-	23.70	25159151
1021 (in isoform 2)	Phosphorylation	-	20.84	25159151
1023	Phosphorylation	RLSISAESQSPGTSM EEEEEEECCCCCCCC	35.36	27251275
1023 (in isoform 2)	Phosphorylation	-	35.36	25159151
1025	Phosphorylation	SISAESQSPGTSMTP EEEEECCCCCCCCCC	34.28	28348404
1028	Phosphorylation	AESQSPGTSMTPSSG EECCCCCCCCCCCCC	20.79	28348404
1029	Phosphorylation	ESQSPGTSMTPSSGS ECCCCCCCCCCCCCC	26.95	28348404
1031	Phosphorylation	QSPGTSMTPSSGSFP CCCCCCCCCCCCCCC	21.56	28348404
1033	Phosphorylation	PGTSMTPSSGSFPSA CCCCCCCCCCCCCCC	37.86	19764811
1034	Phosphorylation	GTSMTPSSGSFPSAY CCCCCCCCCCCCCCC	39.39	19764811
1036	Phosphorylation	SMTPSSGSFPSAYDQ CCCCCCCCCCCCCCC	35.61	19764811
1049	Phosphorylation	DQQSSKDSRQGQWQR CCCCCCCCCCCHHHH	30.18	-
1113	Phosphorylation	KFSVHVESVLNRVPQ EEEEEHHHHHHCCCC	30.71	-
1135	Phosphorylation	VEAILVLTMLADIEI HHHHHHHHHHHCCEE	11.58	-
1144	Phosphorylation	LADIEIHSIGSIIAV HHCCEECCHHHHHHH	34.01	-
1174	Phosphorylation	KTLGADDTMLAKDPA HHCCCCCCCCCCCCC	18.03	24719451
1175	Sulfoxidation	TLGADDTMLAKDPAS HCCCCCCCCCCCCCC	4.39	21406390
1220	Farnesylation	EFLPHSICAMQ---- HHCCHHHHCCC----	2.64	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
972	S	Phosphorylation	Kinase	PHK-FAMILY	-	GPS
972	S	Phosphorylation	Kinase	PHK_GROUP	-	PhosphoELM
985	S	Phosphorylation	Kinase	PHK-FAMILY	-	GPS
985	S	Phosphorylation	Kinase	PHK_GROUP	-	PhosphoELM
1007	S	Phosphorylation	Kinase	PHK-FAMILY	-	GPS
1007	S	Phosphorylation	Kinase	PHK_GROUP	-	PhosphoELM
1018	S	Phosphorylation	Kinase	PHK-FAMILY	-	GPS
1018	S	Phosphorylation	Kinase	PKA	-	Uniprot
1018	S	Phosphorylation	Kinase	PHK_GROUP	-	PhosphoELM

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of KPB1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KPB1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SGTA_HUMAN	SGTA	physical	22939629
VAC14_HUMAN	VAC14	physical	22939629

Drug and Disease Associations

• Kegg Disease
• H00069: Glycogen storage diseases (GSD), including: von Gierke disease (GSD type Ia); Pompe disease (GSD typ
• OMIM Disease
• 300559: Glycogen storage disease 9D (GSD9D)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-735, AND MASSSPECTROMETRY.
PubMed: 18691976