KLKB1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KLKB1_HUMAN
• UniProt AC: P03952
• Protein Name: Plasma kallikrein
• Gene Name: KLKB1
• Organism: Homo sapiens (Human).
• Sequence Length: 638
• Subcellular Localization: Secreted.
• Protein Description: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin..
• Protein Sequence: MILFKQATYFISLFATVSCGCLTQLYENAFFRGGDVASMYTPNAQYCQMRCTFHPRCLLFSFLPASSINDMEKRFGCFLKDSVTGTLPKVHRTGAVSGHSLKQCGHQISACHRDIYKGVDMRGVNFNVSKVSSVEECQKRCTNNIRCQFFSYATQTFHKAEYRNNCLLKYSPGGTPTAIKVLSNVESGFSLKPCALSEIGCHMNIFQHLAFSDVDVARVLTPDAFVCRTICTYHPNCLFFTFYTNVWKIESQRNVCLLKTSESGTPSSSTPQENTISGYSLLTCKRTLPEPCHSKIYPGVDFGGEELNVTFVKGVNVCQETCTKMIRCQFFTYSLLPEDCKEEKCKCFLRLSMDGSPTRIAYGTQGSSGYSLRLCNTGDNSVCTTKTSTRIVGGTNSSWGEWPWQVSLQVKLTAQRHLCGGSLIGHQWVLTAAHCFDGLPLQDVWRIYSGILNLSDITKDTPFSQIKEIIIHQNYKVSEGNHDIALIKLQAPLNYTEFQKPICLPSKGDTSTIYTNCWVTGWGFSKEKGEIQNILQKVNIPLVTNEECQKRYQDYKITQRMVCAGYKEGGKDACKGDSGGPLVCKHNGMWRLVGITSWGEGCARREQPGVYTKVAEYMDWILEKTQSSDGKAQMQSPA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
109	Phosphorylation	KQCGHQISACHRDIY HHHCCCCHHHCHHHH	20.48	29052541
125	N-linked_Glycosylation	GVDMRGVNFNVSKVS CCCCCCCCCCEECCC	26.12	17623646
125	N-linked_Glycosylation	GVDMRGVNFNVSKVS CCCCCCCCCCEECCC	26.12	-
127	N-linked_Glycosylation	DMRGVNFNVSKVSSV CCCCCCCCEECCCCH	31.76	18638581
127	N-linked_Glycosylation	DMRGVNFNVSKVSSV CCCCCCCCEECCCCH	31.76	3521732
175	Phosphorylation	LKYSPGGTPTAIKVL EEECCCCCCCCEEHH	24.31	23403867
177	Phosphorylation	YSPGGTPTAIKVLSN ECCCCCCCCEEHHHC	40.63	23403867
260	Phosphorylation	RNVCLLKTSESGTPS CCEEEEEECCCCCCC	37.38	30576142
261	Phosphorylation	NVCLLKTSESGTPSS CEEEEEECCCCCCCC	28.33	24505115
263	Phosphorylation	CLLKTSESGTPSSST EEEEECCCCCCCCCC	47.97	24505115
269	Phosphorylation	ESGTPSSSTPQENTI CCCCCCCCCCCCCCC	48.74	30576142
275	Phosphorylation	SSTPQENTISGYSLL CCCCCCCCCCCEEEE	18.77	-
283	Phosphorylation	ISGYSLLTCKRTLPE CCCEEEEEEECCCCC	22.67	30576142
308	N-linked_Glycosylation	DFGGEELNVTFVKGV CCCCCEEEEEEEECC	33.78	3521732
308	N-linked_Glycosylation	DFGGEELNVTFVKGV CCCCCEEEEEEEECC	33.78	17623646
371	Phosphorylation	TQGSSGYSLRLCNTG CCCCCCEEEEEEECC	15.56	24719451
384	O-linked_Glycosylation	TGDNSVCTTKTSTRI CCCCCEEEECCCCEE	29.22	OGP
396	N-linked_Glycosylation	TRIVGGTNSSWGEWP CEEECCCCCCCCCCC	37.00	3521732
396	N-linked_Glycosylation	TRIVGGTNSSWGEWP CEEECCCCCCCCCCC	37.00	19159218
453	N-linked_Glycosylation	RIYSGILNLSDITKD HHHHCCCCHHHCCCC	35.26	17623646
453	N-linked_Glycosylation	RIYSGILNLSDITKD HHHHCCCCHHHCCCC	35.26	18638581
494	N-linked_Glycosylation	IKLQAPLNYTEFQKP EEEECCCCCCCCCCC	40.39	3521732
494	N-linked_Glycosylation	IKLQAPLNYTEFQKP EEEECCCCCCCCCCC	40.39	18638581
506	Phosphorylation	QKPICLPSKGDTSTI CCCEECCCCCCCCEE	36.00	24719451
510	Phosphorylation	CLPSKGDTSTIYTNC ECCCCCCCCEEEECC	36.12	19690332
511	Phosphorylation	LPSKGDTSTIYTNCW CCCCCCCCEEEECCE	19.97	19690332
514	Phosphorylation	KGDTSTIYTNCWVTG CCCCCEEEECCEECC	7.62	19690332
515	Phosphorylation	GDTSTIYTNCWVTGW CCCCEEEECCEECCC	21.76	19690332
520	Phosphorylation	IYTNCWVTGWGFSKE EEECCEECCCCCCCC	11.61	19690332
525	Phosphorylation	WVTGWGFSKEKGEIQ EECCCCCCCCCCHHH	35.65	19690332
604	Methylation	SWGEGCARREQPGVY ECCCCCCCCCCCCCC	46.90	24381453
605	Methylation	WGEGCARREQPGVYT CCCCCCCCCCCCCCH	28.51	24381461

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of KLKB1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of KLKB1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KLKB1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KNG1_HUMAN	KNG1	physical	7944388
GEPH_HUMAN	GPHN	physical	28514442
ZBTB1_HUMAN	ZBTB1	physical	28514442
CND2_HUMAN	NCAPH	physical	28514442
CNOT2_HUMAN	CNOT2	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 612423: Prekallikrein deficiency (PKK deficiency)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

N-linked Glycosylation

"Glycoproteomics analysis of human liver tissue by combination ofmultiple enzyme digestion and hydrazide chemistry.";
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
J. Proteome Res. 8:651-661(2009).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-308; ASN-396; ASN-453 ANDASN-494, AND MASS SPECTROMETRY.
PubMed: 19159218

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction,hydrazide chemistry, and mass spectrometry.";
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E.,Moore R.J., Smith R.D.;
J. Proteome Res. 4:2070-2080(2005).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-127; ASN-308; ASN-396;ASN-453 AND ASN-494, AND MASS SPECTROMETRY.
PubMed: 16335952

"Identification and quantification of N-linked glycoproteins usinghydrazide chemistry, stable isotope labeling and mass spectrometry.";
Zhang H., Li X.-J., Martin D.B., Aebersold R.;
Nat. Biotechnol. 21:660-666(2003).
Cited for: GLYCOSYLATION AT ASN-453.
PubMed: 12754519

"Human plasma prekallikrein, a zymogen to a serine protease thatcontains four tandem repeats.";
Chung D.W., Fujikawa K., McMullen B.A., Davie E.W.;
Biochemistry 25:2410-2417(1986).
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
PubMed: 3521732