KKCC2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KKCC2_MOUSE
• UniProt AC: Q8C078
• Protein Name: Calcium/calmodulin-dependent protein kinase kinase 2
• Gene Name: Camkk2
• Organism: Mus musculus (Mouse).
• Sequence Length: 588
• Subcellular Localization: Nucleus. Cytoplasm. Cell projection. Predominantly nuclear in unstimulated cells. Found in the cytoplasm and neurites after forskolin induction..
• Protein Description: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK4 and CAMK1D (By similarity). Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). May play a role in neurite growth. Isoform 2 may promote neurite elongation, while isoform 1 may promoter neurite branching (By similarity). May be involved in hippocampal activation of CREB1..
• Protein Sequence: MSSCVSSQPTSDRVAPQDELGSGGGSREGQKPCEALRGLSSLSIHLGMESFIVVTECEPGRGVDLNLARDQPPEADGQELPLEASDPESRSPLSGRKMSLQEPSQGGPASSSNSLDMNGRCICPSLSYSPASSPQSSPRMPRRPTVESHHVSITGLQDCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRRPPPRGARPAPGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPTLKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSNEFKGSDALLSNTVGTPAFMAPESLSETRKIFSGKALDVWAMGVTLYCFVFGQCPFMDERIMCLHSKIKSQALEFPDQPDIAEDLKDLITRMLDKNPESRIVVPEIKLHPWVTRHGAEPLPSEDENCTLVEVTEEEVENSVKHIPSLATVILVKTMIRKRSFGNPFEGSRREERSLSAPGNLLTKKPTREWEPLSEPKEARQRRQPPGPRAGPCGGGGSALVKGGPCVESWGAPAPGSPPRMPPLQPEEVMEPE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSSCVSSQP ------CCCCCCCCC	32.23	-
85	Phosphorylation	QELPLEASDPESRSP CCCCCCCCCCCCCCC	44.30	21082442
89	Phosphorylation	LEASDPESRSPLSGR CCCCCCCCCCCCCCC	43.37	26824392
91	Phosphorylation	ASDPESRSPLSGRKM CCCCCCCCCCCCCCC	39.20	25521595
94	Phosphorylation	PESRSPLSGRKMSLQ CCCCCCCCCCCCCCC	39.68	25619855
99	Phosphorylation	PLSGRKMSLQEPSQG CCCCCCCCCCCCCCC	29.90	25521595
104	Phosphorylation	KMSLQEPSQGGPASS CCCCCCCCCCCCCCC	40.48	25619855
110	Phosphorylation	PSQGGPASSSNSLDM CCCCCCCCCCCCCCC	37.12	25619855
111	Phosphorylation	SQGGPASSSNSLDMN CCCCCCCCCCCCCCC	35.33	25619855
112	Phosphorylation	QGGPASSSNSLDMNG CCCCCCCCCCCCCCC	28.13	25619855
114	Phosphorylation	GPASSSNSLDMNGRC CCCCCCCCCCCCCEE	27.91	25619855
125	Phosphorylation	NGRCICPSLSYSPAS CCEEECCCCCCCCCC	26.07	26643407
127	Phosphorylation	RCICPSLSYSPASSP EEECCCCCCCCCCCC	28.09	26745281
128	Phosphorylation	CICPSLSYSPASSPQ EECCCCCCCCCCCCC	24.78	26745281
129	Phosphorylation	ICPSLSYSPASSPQS ECCCCCCCCCCCCCC	15.60	21082442
132	Phosphorylation	SLSYSPASSPQSSPR CCCCCCCCCCCCCCC	46.03	21082442
133	Phosphorylation	LSYSPASSPQSSPRM CCCCCCCCCCCCCCC	29.12	25521595
136	Phosphorylation	SPASSPQSSPRMPRR CCCCCCCCCCCCCCC	45.77	25168779
137	Phosphorylation	PASSPQSSPRMPRRP CCCCCCCCCCCCCCC	15.98	21082442
154	Phosphorylation	ESHHVSITGLQDCVQ CCCCCEEECHHHHHH	25.08	29899451
176	Phosphorylation	DEIGKGSYGVVKLAY CCCCCCCCEEEEEEE	23.70	-
190	Phosphorylation	YNENDNTYYAMKVLS EECCCCCEEEEHHHC	8.76	-
191	Phosphorylation	NENDNTYYAMKVLSK ECCCCCEEEEHHHCH	9.74	-
223	Glutathionylation	ARPAPGGCIQPRGPI CCCCCCCCCCCCCHH	3.02	24333276
456	Phosphorylation	HGAEPLPSEDENCTL CCCCCCCCCCCCCEE	66.42	-
462	Phosphorylation	PSEDENCTLVEVTEE CCCCCCCEEEECCHH	45.48	29899451
474	Phosphorylation	TEEEVENSVKHIPSL CHHHHHHHHCCCCHH	21.02	-
483	Phosphorylation	KHIPSLATVILVKTM CCCCHHHHHHHHHHH	17.24	21183079
489	Phosphorylation	ATVILVKTMIRKRSF HHHHHHHHHHHHHCC	15.45	-
495	Phosphorylation	KTMIRKRSFGNPFEG HHHHHHHCCCCCCCC	40.31	25521595
503	Phosphorylation	FGNPFEGSRREERSL CCCCCCCCCCHHHCC	22.44	28833060
509	Phosphorylation	GSRREERSLSAPGNL CCCCHHHCCCCCCCC	29.92	22324799
511	Phosphorylation	RREERSLSAPGNLLT CCHHHCCCCCCCCCC	33.39	22942356
511 (in isoform 2)	Phosphorylation	-	33.39	27566939
518	Phosphorylation	SAPGNLLTKKPTREW CCCCCCCCCCCCCCC	39.94	28833060
522	Phosphorylation	NLLTKKPTREWEPLS CCCCCCCCCCCCCCC	50.53	24719451
522 (in isoform 2)	Phosphorylation	-	50.53	25266776
525 (in isoform 2)	Phosphorylation	-	14.82	30635358
564	Phosphorylation	KGGPCVESWGAPAPG ECCCCCCCCCCCCCC	15.48	25619855
572	Phosphorylation	WGAPAPGSPPRMPPL CCCCCCCCCCCCCCC	30.58	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of KKCC2_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of KKCC2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KKCC2_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of KKCC2_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129 AND SER-133, ANDMASS SPECTROMETRY.
PubMed: 17242355

"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations.";
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
Mol. Cell. Proteomics 6:283-293(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495, AND MASSSPECTROMETRY.
PubMed: 17114649